“…Nano-sized fillers can be either inorganic or organic materials, such as clay (e.g., montmorillonite), natural antimicrobial agents (e.g., nisin), metal (e.g., silver, gold), and metal oxides (e.g., zinc oxide (ZnO), titanium dioxide (TiO 2 )), to be the material of choice due to its antimicrobial activity, thermal stability and ability to improve mechanical properties of biopolymer films [147,149,153,[155][156][157]. Chitosan-ZnO hybrid coatings on polyethylene films have been reported to reduce growth of pathogenic bacteria and fungi [85], as well as increase the shelf life of okra (Abelmoschus esculentus) vegetables [158]. Zhang et al developed a chitosan-TiO 2 composite film for the packaging of red grapes and evaluated antimicrobial activity against food-borne pathogenic microbes, namely E. coli, S. aureus, Candida albicans, and Aspergillus niger [147].…”