Nanocomposite of polymerized ionic liquid and graphene used as modifier for direct electrochemistry of cytochrome c and nitric oxide biosensing

Chen, Hua-Mao; Zhao, Guang‐Chao

doi:10.1007/s10008-012-1769-5

Cited by 28 publications

(26 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This value corresponded well with data reported for immobilized Cyt c [46]. The rate constant for the MIP bound Cyt c is considerably higher that the values reported recently for a screen-printed electrode modified with didodecyldimethyl ammonium bromide and carbon black of 0.93-1.22 s À1 [47] or an electrode covered with a ionic liquid/graphene nanocomposite of 2.39 s À1 [48]. The binding was reversible, though, as 80 % of the adsorbed Cyt c (assessed by the peak areas of the corresponding redox signals) dissociated within 20 min of incubation in phosphate buffer without Cyt c. Based on these observations, it was justified to assume that the peak area of the respective redox peaks in CV experiment reflected the surface concentrations of adsorbed, properly oriented and native Cyt c at various bulk concentrations of Cyt c. The surface concentration for both the MUA-MIP and the MUA-NIP-covered electrodes increased almost linearly, approaching saturation above 4 mmol L…”

Section: Template Removal and Rebindingsupporting

confidence: 91%

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

et al. 2015

View full text Add to dashboard Cite

Hybrid architectures which combine a MIP with an immobilized “affinity ligand” or a biocatalyst sum up the advantages of both components. In this paper, hybrid architectures combining a layer of a molecularly imprinted electropolymer with a mini‐enzyme or a self‐assembled monolayer will be presented. (i) Microperoxidase‐11 (MP‐11) catalyzed oxidation of the drug aminopyrine on a product‐imprinted sublayer: The peroxide dependent conversion of the analyte aminopyrine takes place in the MP‐11 containing layer on top of a product‐imprinted electropolymer on the indicator electrode. The hierarchical architecture resulted in the elimination of interfering signals for ascorbic acid and uric acid. An advantage of the new hierarchical structure is the separation of MIP formation by electropolymerization and immobilization of the catalyst. In this way it was for the first time possible to integrate an enzyme with a MIP layer in a sensor configuration. This combination has the potential to be transferred to other enzymes, e.g. P450, opening the way to clinically important analytes. (ii) Epitope‐imprinted poly‐scopoletin layer for binding of the C‐terminal peptide and cytochrome c (Cyt c): The MIP binds both the target peptide and the parent protein almost eight times stronger than the non‐imprinted polymer with affinities in the lower micromolar range. Exchange of only one amino acid in the peptide decreases the binding by a factor of five. (iii) MUA‐poly‐scopoletin MIP for cytochrome c: Cyt c bound to the MIP covered gold electrode exhibits direct electron transfer with a redox potential and rate constant typical for the native protein. The MIP cover layer suppresses the displacement of the target protein by BSA or myoglobin. The combination of protein imprinted polymers with an efficient electron transfer is a new concept for characterizing electroactive proteins such as Cyt c. The competition with other proteins shows that the MIP binds its target Cyt c preferentially and that molecular shape and the charge of protein determine the binding of interfering proteins.

show abstract

Section: Template Removal and Rebindingsupporting

confidence: 91%

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The determined parameters of this sensor and other modified electrodes used for the determination of NO are listed in Table 1. Compared with the existing reports [39-45], this method to prepare a Hb/GNPs-GR-SDS/BPG modified electrode with high sensitivity, wide linear range and good repeatability is convenient.…”

Section: Resultsmentioning

confidence: 98%

Direct Electrochemistry of Hemoglobin at a Graphene Gold Nanoparticle Composite Film for Nitric Oxide Biosensing

Zhao

2013

Sensors

View full text Add to dashboard Cite

A simple two-step method was employed for preparing nano-sized gold nanoparticles-graphene composite to construct a GNPs-GR-SDS modified electrode. Hemoglobin (Hb) was successfully immobilized on the surface of a basal plane graphite (BPG) electrode through a simple dropping technique. Direct electrochemistry and electrocatalysis of the hemoglobin-modified electrode was investigated. The as-prepared composites showed an obvious promotion of the direct electro-transfer between hemoglobin and the electrode. A couple of well-defined and quasi-reversible Hb CV peaks can be observed in a phosphate buffer solution (pH 7.0). The separation of anodic and cathodic peak potentials is 81 mV, indicating a fast electron transfer reaction. The experimental results also clarified that the immobilized Hb retained its biological activity for the catalysis toward NO. The biosensor showed high sensitivity and fast response upon the addition of NO, under the conditions of pH 7.0, potential -0.82 V. The time to reach the stable-state current was less than 3 s, and the linear response range of NO was 0.72-7.92 μM, with a correlation coefficient of 0.9991.

show abstract

“…In addition, the substrate diffusion should take place easily. The employed approaches for preparation of third‐generation biosensors for NO detection (Table ) are mainly based on adsorption (physical and layer‐by‐layer ), entrapment or covalent bonding . Each method possesses its particular benefits and disadvantages.…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

“…Each method possesses its particular benefits and disadvantages. Adsorption have been mostly used due to its simplicity and because loss of activity is not significant . However, leaching of the biocomponent has been reported as a problem due to the weak Van der Waal′s forces and electrostatic or hydrophobic interactions established .…”

Section: Direct Electron Transfer Behavior Of Heme Proteins and Porphmentioning

confidence: 99%

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

et al. 2018

View full text Add to dashboard Cite

Nitric oxide radical (NO) is a signalling molecule involved in virtually all forms of life. Its relevance has been leading to the development of different analytical methodologies to assess the temporal and spatial fluxes of NO under the complex biological milieu. Third‐generation electrochemical biosensors are promising tools for in loco and in vivo NO quantification and, over the past years, heme proteins and porphyrins have been used in their design. Since there are some limitations with the biorecognition element directly adsorbed onto the electrode surface, nanomaterials (carbon nanotubes, gold nanoparticles, etc.) and polymers (cellulose, chitosan, nafion®, polyacrylamide, among others) have been explored to achieve high kinetics and better biosensor performance. In this review, a broad overview of the field of electrochemical third‐generation biosensors for NO electroanalysis is presented, discussing their main characteristics and aiming new outlooks and advances in this field.

show abstract

Nanocomposite of polymerized ionic liquid and graphene used as modifier for direct electrochemistry of cytochrome c and nitric oxide biosensing

Cited by 28 publications

References 55 publications

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

Cytochrome c‐Derived Hybrid Systems Based on Moleculary Imprinted Polymers

Direct Electrochemistry of Hemoglobin at a Graphene Gold Nanoparticle Composite Film for Nitric Oxide Biosensing

Nitric Oxide Detection Using Electrochemical Third‐generation Biosensors – Based on Heme Proteins and Porphyrins

Contact Info

Product

Resources

About