Abstract:Bioprinting aims to provide new avenues for regenerating damaged human tissues through the controlled printing of live cells and biocompatible materials that can function therapeutically. Polymeric hydrogels are commonly investigated ink materials for 3D and 4D bioprinting applications, as they can contain intrinsic properties relative to those of the native tissue extracellular matrix and can be printed to produce scaffolds of hierarchical organization. The incorporation of nanoscale material additives, such … Show more
“…The incorporation of contrast agents into the hydrogel solutions has been established as an effective method to tailor various physiomechanical properties of these scaffolding biomaterials to adapt for a variety of biomedical applications. [19,[64][65][66] To examine the effect of incorporated molecular and NP contrast agents with different sizes on the mechanical properties of bioprints, microindentation test was performed on simple cubic constructs printed using various inks (Figure 2G). In comparison to the control 12% (blank) GelMA group, the addition of molecular agents, that is, Gd chelate and Iod both in bare form and loaded in liposome nanocapsules, resulted in significant increases in the elastic modulus of bioprints.…”
Abstract3D Bioprinting is revolutionizing the fields of personalized and precision medicine by enabling the manufacturing of bioartificial implants that recapitulate the structural and functional characteristics of native tissues. However, the lack of quantitative and noninvasive techniques to longitudinally track the function of implants has hampered clinical applications of bioprinted scaffolds. In this study, multimaterial 3D bioprinting, engineered nanoparticles (NPs), and spectral photon‐counting computed tomography (PCCT) technologies were integrated for the aim of developing a new precision medicine approach to custom‐engineer scaffolds with traceability. Multiple CT‐visible hydrogel‐based bioinks, containing distinct molecular (iodine and gadolinium) and NP (iodine‐loaded liposome, gold, methacrylated gold (AuMA), and Gd2O3) contrast agents, were used to bioprint scaffolds with varying geometries at adequate fidelity levels. In vitro release studies, together with printing fidelity, mechanical, and biocompatibility tests identified AuMA and Gd2O3 NPs as optimal reagents to track bioprinted constructs. Spectral PCCT imaging of scaffolds in vitro and subcutaneous implants in mice enabled noninvasive material discrimination and contrast agent quantification. Together, these results establish a novel theranostic platform with high precision, tunability, throughput, and reproducibility and open new prospects for a broad range of applications in the field of precision and personalized regenerative medicine.This article is protected by copyright. All rights reserved
“…The incorporation of contrast agents into the hydrogel solutions has been established as an effective method to tailor various physiomechanical properties of these scaffolding biomaterials to adapt for a variety of biomedical applications. [19,[64][65][66] To examine the effect of incorporated molecular and NP contrast agents with different sizes on the mechanical properties of bioprints, microindentation test was performed on simple cubic constructs printed using various inks (Figure 2G). In comparison to the control 12% (blank) GelMA group, the addition of molecular agents, that is, Gd chelate and Iod both in bare form and loaded in liposome nanocapsules, resulted in significant increases in the elastic modulus of bioprints.…”
Abstract3D Bioprinting is revolutionizing the fields of personalized and precision medicine by enabling the manufacturing of bioartificial implants that recapitulate the structural and functional characteristics of native tissues. However, the lack of quantitative and noninvasive techniques to longitudinally track the function of implants has hampered clinical applications of bioprinted scaffolds. In this study, multimaterial 3D bioprinting, engineered nanoparticles (NPs), and spectral photon‐counting computed tomography (PCCT) technologies were integrated for the aim of developing a new precision medicine approach to custom‐engineer scaffolds with traceability. Multiple CT‐visible hydrogel‐based bioinks, containing distinct molecular (iodine and gadolinium) and NP (iodine‐loaded liposome, gold, methacrylated gold (AuMA), and Gd2O3) contrast agents, were used to bioprint scaffolds with varying geometries at adequate fidelity levels. In vitro release studies, together with printing fidelity, mechanical, and biocompatibility tests identified AuMA and Gd2O3 NPs as optimal reagents to track bioprinted constructs. Spectral PCCT imaging of scaffolds in vitro and subcutaneous implants in mice enabled noninvasive material discrimination and contrast agent quantification. Together, these results establish a novel theranostic platform with high precision, tunability, throughput, and reproducibility and open new prospects for a broad range of applications in the field of precision and personalized regenerative medicine.This article is protected by copyright. All rights reserved
“…Electrospun poly(vinyl alcohol))/graphene nanocomposite nanofibers [151,152] and poly(D, L-lactic-co-glycolic acid)/graphene oxide nanocomposite nanofibers have been observed for the fabrication of tissue engineering scaffolds [153,154]. However, this field demands the explorations of more innovative design combinations of biodegradable polymers and nanocarbon nanoparticles [155,156]. The anti-microbial Some further applications of polymer/carbon nanoparticle nanocomposite-resultant nanofibers include electronics, radiation shielding, and biomedical arenas [134].…”
Section: Significance Of Electrospun Polymer/nanocarbon Nanocomposite...mentioning
Polymeric nanofibers have emerged as exclusive one-dimensional nanomaterials. Various polymeric nanofibers and nanocomposite nanofibers have been processed using the thermoplastic, conducting, and thermoset matrices. This review aims to highlight the worth of electrospinning technology for the processing of polymer/nanocarbon nanocomposite nanofibers. In this regard, the design, morphology, physical properties, and applications of the nanofibers were explored. The electrospun polymer/nanocarbon nanofibers have a large surface area and fine fiber orientation, alignment, and morphology. The fiber processing technique and parameters were found to affect the nanofiber morphology, diameter, and essential physical features such as electrical conductivity, mechanical properties, thermal stability, etc. The polymer nanocomposites with nanocarbon nanofillers (carbon nanotube, graphene, fullerene, etc.) were processed into high-performance nanofibers. Successively, the electrospun nanocomposite nanofibers were found to be useful for photovoltaics, supercapacitors, radiation shielding, and biomedical applications (tissue engineering, antimicrobials, etc.).
“…However, the presence of NPs can also lead to some adverse effects, such as reduced biocompatibility and slower degradation rates. The main reason for the advancement of 3D bioprinting technology is the goal of increasing its importance and possibly reducing its negative impact by improving the manufacturing process of bio-functional nanocomposites [ 75 , 143 , 144 ]. The NPs to be incorporated can be from carbon based NPs such as graphene, graphene oxide, carbon nanotubes, and carbon nanofibers; ceramic NPs such as silica-based nano biomaterials, bioactive glasses, and calcium phosphate NPs; biopolymeric nanoparticles, and various metallic NPs [ [145] , [146] , [147] , [148] ].…”
Section: D Printed Drug Loaded Nanomaterials For Wound Healingmentioning
confidence: 99%
“…The various techniques and nanomaterials for nanocomposite fabrication are illustrated in Fig. 4 [ 75 ]. Fig.…”
Section: D Printed Drug Loaded Nanomaterials For Wound Healingmentioning
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