Nanocoating with plant-derived pectins activates osteoblast response in vitro

Folkert, Justyna; Meresta, Anna; Gaber, Timo; Miksch, K.; Buttgereit, Frank; Detert, J.; Pischon, Nicole; Gurzawska, Katarzyna

doi:10.2147/ijn.s99020

Cited by 19 publications

(18 citation statements)

References 28 publications

(68 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several authors have reported that polysaccharides, including pectins, have promoted adhesion and proliferation of cells [12]. Coatings of RG-I derived from plant pectins on tissue culture polystyrene have promoted proliferation of primary osteoblasts and osteoblast-like MC3T3-E1 cells [13]. Conversely, other studies by the same group (Gurwaszka K et al) on different RG-I preparations on titanium surfaces revealed no positive effect or, in some cases, an inhibitory effect of the coatings on the proliferation of SaOS-2 osteoblast-like cells [8].…”

Section: Resultsmentioning

confidence: 99%

Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

et al. 2018

View full text Add to dashboard Cite

Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a prerequisite .. Hence, surface coatings which promote cell adhesion and proliferation are desirable. Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue. Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation. Highlights  Ti6Al4V discs were prepared by additive manufacturing (EBM)  Discs were coated with pectins from citrus (C) and apple (A)  Coatings also contained the enzyme alkaline phosphatase (ALP)  Coatings promoted proliferation of human bone marrow stromal cells (hBMSC)  C-ALP coatings promoted proliferation more than A-ALP coatings *Highlights (for review)

show abstract

Section: Resultsmentioning

confidence: 99%

Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Furthermore, the RG‐I molecules are not enzymatically degraded in the body and their structure is relatively easy to control . Our results showed that the RG‐I properties may have a great effect not only on osteoblasts, but also on fibroblasts during the bone healing process due to increasing expression levels of COL1A1 and FN1 (Fig. ).…”

Section: Discussionmentioning

confidence: 71%

“…Recently, it was reported that pectin polysaccharide rhamnogalacturonan‐I (RG‐I) can prevent inflammation, and improve tissue regeneration . Our previous studies also showed that nanocoated RG‐I pectins enhance bone tissue regeneration in vitro through upregulation of the gene expression of osteogenic markers in human and murine osteoblasts . Moreover, pectin polysaccharides have been reported to affect the behavior of fibroblasts, the most common cells of connective tissue, which play a critical role in the soft and bone tissue healing process .…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14][15][16] Our previous studies also showed that nanocoated RG-I pectins enhance bone tissue regeneration in vitro through upregulation of the gene expression of osteogenic markers in human and murine osteoblasts. [17][18][19] Moreover, pectin polysaccharides have been reported to affect the behavior of fibroblasts, the most common cells of connective tissue, which play a critical role in the soft and bone tissue healing process. 20 RG-I is able to induce adhesion, proliferation and survival of fibroblasts and may stimulate soft and hard tissue regeneration as a result.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Mieszkowska

Folkert

Gaber

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

Implant failures are primarily related to bacterial infections and inflammation. Nanocoating of implant devices with organic molecules is a method used for improving their integration into host tissues and limiting inflammation. Bioengineered plant-derived rhamnogalacturonan-Is (RG-Is) from pectins improve tissue regeneration and exhibit anti-inflammatory properties. Therefore, the aim of this study is to evaluate the in vitro effect of RG-I nanocoating on human gingival primary fibroblast (HGF) activity and proinflammatory response following Porphyromonas gingivalis (P. gingivalis) infection. Infected HGFs were incubated on tissue culture polystyrene (TCPS) plates coated with unmodified RG-I isolated from potato pectin (PU) and dearabinanated RG-I (PA). HGF morphology, proliferation, metabolic activity, and expression of genes responsible for extracellular matrix (ECM) turnover and proinflammatory response were examined. Following the P. gingivalis infection, PU and PA significantly promoted HGF proliferation and metabolic activity. Moreover, gene expression levels of IL1B, IL8, TNFA, and MMP2 decreased in the infected cells cultured on PU and PA, whereas the expression of COL1A1, FN1, and FGFR1 was upregulated. The results indicate that RG-Is are promising candidates for nanocoating of an implant surface, can reduce inflammation, and enhance implant integration, particularly in medically compromised patients with chronic inflammatory diseases such as periodontitis and rheumatoid arthritis. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3475-3481, 2017.

show abstract

“…In our previous studies investigating the in vitro effect of plant-derived polysaccharides coatings, we have shown that the expression of osteoclastogenesis marker RANKL corresponds with the expression of osteogenic markers ( COL1A1 , ALPL and BGLAP ) in murine primary calvarial osteoblasts and the pre-osteoblast cell line MC3T3-E1 at different stages of osteoblast differentiation. Moreover the expression of COL1A1 , ALPL , BGLAP, and RANKL in cultured osteoblasts was shown to increase with cell differentiation, suggesting that mature and differentiated osteoblasts support osteoclast activation through increased RANKL expression [ 32 , 33 ]. According to literature, the differentiated osteoblast cells promote osteoclastogenesis to a greater extent than undifferentiated osteoblasts [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Phenolic-Enriched Collagen Fibrillar Coatings on Titanium Alloy to Promote Osteogenic Differentiation and Reduce Inflammation

Mieszkowska

Beaumont

Martocq

et al. 2020

IJMS

View full text Add to dashboard Cite

The adsorption of biomolecules on biomaterial surfaces can promote their integration with surrounding tissue without changing their bulk properties. For biomaterials in bone reconstruction, the promotion of osteogenic differentiation and reduction of inflammation are desirable. Fibrillar coatings are interesting because of fibrils’ high surface area-volume ratio, aiding adsorption and adhesion. Fibrils also serve as a matrix for the immobilization of biomolecules with biological activity, such as the phenolic compound phloroglucinol (PG), the subunit of marine polyphenols. The aim of this work was to investigate the influence of PG coatings on fibroblast- and osteoblast-like cells to increase the osseointegration of titanium implants. Collagen fibril coatings, containing PG at low and high concentrations, were produced on titanium alloy (Ti6Al4V) scaffolds generated by additive manufacturing (AM). These coatings, especially PG-enriched coatings, reduced hydrophobicity and modulated the behavior of human osteosarcoma SaOS-2 and mouse embryonic fibroblast 3T3 cell lines. Both osteoblastic and fibroblastic cells spread and adhered well on PG-enriched coatings. Coatings significantly reduced the inflammatory response. Moreover, osteogenic differentiation was promoted by collagen coatings with a high PG concentration. Thus, the enrichment of collagen fibril coatings with PG is a promising strategy to improve Ti6Al4V implants for bone contact in orthopedics and dentistry and is worthy of further investigation.

show abstract

Nanocoating with plant-derived pectins activates osteoblast response in vitro

Cited by 19 publications

References 28 publications

Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation

Pectin nanocoating reduces proinflammatory fibroblast response to bacteria

Phenolic-Enriched Collagen Fibrillar Coatings on Titanium Alloy to Promote Osteogenic Differentiation and Reduce Inflammation

Contact Info

Product

Resources

About