Nanocavity electrode array for recording from electrogenic cells

Hofmann, Boris; Kätelhön, Enno; Schottdorf, Manuel; Offenhäusser, Andreas; Wolfrum, Bernhard

doi:10.1039/c0lc00582g

Cited by 44 publications

(57 citation statements)

References 37 publications

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“…We explain the excellent sealing of the electrode by the small opening which can be covered completely by the cell. Electron microscopy studies of the cell-chip interface reveal that the cell membrane seals the opening very well [24]. It did not escape out attention that we have not found any signals which stand out with their potassium contributions although we find examples for strong calcium and strong sodium currents.…”

Section: A Propagation Of Action Potentialsmentioning

confidence: 63%

“…HL-1 cells generate action potentials spontaneously, pervading the entire cell population [32]. We have cultured cells and seeded them onto chips as previously described [24]. Briefly, the cells were kept in a T25 flask with a supplemented Claycomb medium (supplemented with 10% FBS, 100 μgml −1 penicillin-streptomycin, 0.1 mM norepinephrine, and 2 mM L-glutamine) in an incubator at 37 • C. After coalescing of the the cells to a confluent layer they were passaged using standard procedures.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…A detailed description of the fabrication process is given elsewhere [24]. Briefly, gold electrodes, feed lines, bond pads, and chromium sacrificial layers were fabricated on siliconsilicon oxide wafers using a two-step optical lithography process.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…However, they often require sophisticated techniques of production or lack mechanical stability and longevity. We have recently introduced a sensor design which includes a fluid-filled nanocavity covering a metal electrode [23,24]. This approach decreases the impedance of the sensor due to an increase of the electrode-electrolyte interface area.…”

Section: Introductionmentioning

confidence: 99%

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Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

et al. 2012

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We present a model to describe the response of chip-based nanocavity sensors during extracellular recording of action potentials. These sensors feature microelectrodes which are embedded in liquid-filled cavities. They can be used for the highly localized detection of electrical signals on a chip. We calculate the sensor's impedance and simulate the propagation of action potentials. Subsequently we apply our findings to analyze cell-chip coupling properties. The results are compared to experimental data obtained from cardiomyocyte-like cells. We show that both the impedance and the modeled action potentials fit the experimental data well. Furthermore, we find evidence for a large seal resistance of cardiomyocytes on nanocavity sensors compared to conventional planar recording systems.

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Section: A Propagation Of Action Potentialsmentioning

confidence: 63%

Section: Experimental Methodsmentioning

confidence: 99%

Section: Experimental Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

et al. 2012

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“…Wolfrum et al overcame this dilemma by creating micropores that interface the cells to larger electrodes. 10,11 A third approach involves decreasing the membrane impedance at the cell-electrode junction. In practice, this involves transiently rapturing the cell membrane by applying a zap voltage to a nanostructured electrode, thus allowing the electrodes to enter the cell and perform pseudo-intracellular recording until the raptured membrane reorganizes.…”

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confidence: 99%

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Matsumura

Yamamoto

Niwano

et al. 2016

Applied Physics Letters

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Electrical signals of neuronal cells can be recorded non-invasively and with a high degree of temporal resolution using multielectrode arrays (MEAs). However, signals that are recorded with these devices are small, usually 0.01%-0.1% of intracellular recordings. Here, we show that the amplitude of neuronal signals recorded with MEA devices can be amplified by covering neuronal networks with an electrically resistive sheet. The resistive sheet used in this study is a monolayer of glial cells, supportive cells in the brain. The glial cells were grown on a collagen-gel film that is permeable to oxygen and other nutrients. The impedance of the glial sheet was measured by electrochemical impedance spectroscopy, and equivalent circuit simulations were performed to theoretically investigate the effect of covering the neurons with such a resistive sheet. Finally, the effect of the resistive glial sheet was confirmed experimentally, showing a 6-fold increase in neuronal signals. This technique feasibly amplifies signals of MEA recordings. V C 2016 AIP Publishing LLC. Microelectrode array (MEA) technology is widely used to record trains of action potentials from cultured neurons, cardiomyocytes, or brain slice preparations. [1][2][3][4] The major advantage of this method lies in its high temporal resolution (>10 kHz) and non-invasiveness. MEA recordings have been used both in fundamental studies, e.g., to analyse activity patterns of cultured neuronal networks, 5,6 and in pharmacological research, for screening lead compounds in vitro. 7,8 However, small signals that can be detected through extracellularly positioned microelectrodes inhibit MEAs from being used in further applications such as studies of subthreshold activity. The amplitude of extracellularly recorded signals is usually in the order of ten to a hundred microvolts, which is 3-4 orders of magnitude lower than the intracellular change of membrane potential in an action potential ($100 mV).Various attempts have been made to overcome this issue. One approach involves increasing the seal resistance between the cell and the electrode. This can be achieved by using nanostructured electrodes instead of planar electrodes that become engulfed by overlying cells. 1,9 A second approach is to decrease the electrode impedance. To record activity from single cells, the use of a smaller electrode is preferable, but this comes at the cost of increased electrode impedance, which decreases the signal. Wolfrum et al. overcame this dilemma by creating micropores that interface the cells to larger electrodes. 10,11 A third approach involves decreasing the membrane impedance at the cell-electrode junction. In practice, this involves transiently rapturing the cell membrane by applying a zap voltage to a nanostructured electrode, thus allowing the electrodes to enter the cell and perform pseudo-intracellular recording until the raptured membrane reorganizes. 1,12,13 In this letter, we propose an alternative approach to increase the neuron-electrode seal for amplifying signals in ex...

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Rapid Prototyping of Ultralow‐Cost, Inkjet‐Printed Carbon Microelectrodes for Flexible Bioelectronic Devices

et al. 2018

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Gaining better understanding of the human brain using chip‐based devices and promoting the recovery of lost biological functionality through implants are long pursued endeavors driven by advances in material science, bioelectronics, and the advancing silicon technology. While conventional bioelectronic and neuroelectronic devices typically rely on cleanroom‐based processing, a rapid prototyping technique is proposed that is based on high‐resolution inkjet printing featuring nanoporous carbon electrodes that yield excellent cell–chip coupling. This study aims to overcome two major limitations of conventional approaches that make the development of neuroelectronic devices very challenging and limit a wider use within the research community as well as industry: high costs and lack of rapid prototyping capabilities. These challenges are addressed with an all‐printed, high‐resolution approach that makes use of flexible polymer substrates and is fabricated on a fully digital printing platform. The manufacturing of a chip consumes less than 60 min and costs a few cents per chip. This study introduces nanoporous carbon as a cell‐interfacing electrode material that features outstanding properties for extracellular recording of action potentials and stimulation indicating that the printed carbon chips have the means to be used as a versatile neuroelectronic tool for in vitro and in vivo studies.

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Nanocavity electrode array for recording from electrogenic cells

Cited by 44 publications

References 37 publications

Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

Frequency-dependent signal transfer at the interface between electrogenic cells and nanocavity electrodes

An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings

Rapid Prototyping of Ultralow‐Cost, Inkjet‐Printed Carbon Microelectrodes for Flexible Bioelectronic Devices

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