Nanocage encapsulation improves antiepileptic efficiency of phenytoin

Zhao, Jie; Ye, Zesen; Yang, Jun; Zhang, Qiang; Shan, Wenjun; Wang, Xiumin; Wang, Zhanxiang; Ye, Shefang; Zhou, Xi; Shao, Zhicheng; Ren, Lei

doi:10.1016/j.biomaterials.2020.119849

Cited by 27 publications

(36 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanoparticles have been used for drug encapsulation and modulation of brain diseases. − Recently, a PEGylated-PLGA nanoparticle loaded with epigallocatechin-3-gallate showed efficacy as a therapeutic agent for TLE, as evidenced by improved seizure pattern . Similarly, the protein nanocages modified with brain target TGN peptide were proved to facilitate phenytoin delivery across the BBB and increase the antiepileptic efficiency of drugs in TLE mice . However, targeted delivery of drugs to local neurons in lesion areas of epilepsy remains a significant challenge.…”

Section: Resultsmentioning

confidence: 99%

“…17 Similarly, the protein nanocages modified with brain target TGN peptide were proved to facilitate phenytoin delivery across the BBB and increase the antiepileptic efficiency of drugs in TLE mice. 21 However, targeted delivery of drugs to local neurons in lesion areas of epilepsy remains a significant challenge. Conjugation of antibodies or antibody fragments with liposomes may allow for efficient targeted drug delivery.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies have focused on the development of targeted delivery systems comprised of ligand-modified liposomes for treatment and diagnosis of central nervous system diseases . Ligand-modified liposomes are an outstanding platform for targeting the brain because they cross the blood-brain barrier and can be loaded with various neuromodulatory substances. , Successful developments of liposomal therapeutics for Alzheimer’s disease, Parkinson’s disease, and brain glioma , have led attempts to regulate and treat epilepsy using liposomes or other nanoformulations. − Recent studies proved that strategies using nanocarriers to deliver antiepileptic drugs could improve the drug permeation across the blood-brain barrier (BBB) and therapeutic efficacy on epileptic symptoms. − However, the complex pathogenesis of epilepsy has resulted in limited success in identifying specific targets for treatment of epilepsy. Lesion sites in TLE are concentrated in the hippocampus that are distributed with pyramidal neurons, granular cells, and interneurons .…”

Section: Introductionmentioning

confidence: 99%

“…16−20 Recent studies proved that strategies using nanocarriers to deliver antiepileptic drugs could improve the drug permeation across the blood-brain barrier (BBB) and therapeutic efficacy on epileptic symptoms. 21 However, the complex pathogenesis of epilepsy has resulted in limited success in identifying specific targets for treatment of epilepsy. Lesion sites in TLE are concentrated in the hippocampus that are distributed with pyramidal neurons, granular cells, and interneurons.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

CB1-Antibody Modified Liposomes for Targeted Modulation of Epileptiform Activities Synchronously Detected by Microelectrode Arrays

Dai

Song

Xie

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Temporal lobe epilepsy (TLE) is a focal, recurrent, and refractory neurological disorder. Therefore, precisely targeted treatments for TLE are greatly needed. We designed anti-CB1 liposomes that can bind to CB1 receptors in the hippocampus to deliver photocaged compounds (ruthenium bipyridine triphenylphosphine γ-aminobutyric acid, RuBi-GABA) in the TLE rats. A 16-channel silicon microelectrode array (MEA) was implanted for simultaneously monitoring electrophysiological signals of neurons. The results showed that anti-CB1 liposomes were larger in size and remained in the hippocampus longer than unmodified liposomes. Following the blue light stimulation, the neural firing rates and the local field potentials of hippocampal neurons were significantly reduced. It is indicated that RuBi-GABA was enriched near hippocampal neurons due to anti-CB1 liposome delivery and photolyzed by optical stimulation, resulting dissociation of GABA to exert inhibitory actions. Furthermore, K-means cluster analysis revealed that the firing rates of interneurons were decreased to a greater extent than those of pyramidal neurons, which may have been a result of the uneven diffusion of RuBi-GABA due to liposomes binding to CB1. In this study, we developed a novel, targeted method to regulate neural electrophysiology in the hippocampus of the TLE rat using antibody-modified nanoliposomes, implantable MEA, and photocaged compounds. This method effectively suppressed hippocampal activities during seizure ictus with high spatiotemporal resolution, which is a crucial exploration of targeted therapy for epilepsy.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

CB1-Antibody Modified Liposomes for Targeted Modulation of Epileptiform Activities Synchronously Detected by Microelectrode Arrays

Dai

Song

Xie

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…Three biofilm‐bearing electrodes of the polarized system were on Days 6, 9, and 12, gently rinsed in a sterile 0.05‐M sodium phosphate (pH=6.8), fixed in a solution of 4% paraformaldehyde overnight, and then dehydrated with increasing concentrations of sucrose (10, 20, and 30%, w/v; Zhao et al, 2020). After the fixation, the biofilm was embedded in an optimal‐cutting‐temperature compound (Sakura 4583; Sakura) and frozen at −80°C.…”

Section: Methodsmentioning

confidence: 99%

Stratified microbial structure and activity within anode biofilm during electrochemically assisted brewery wastewater treatment

Mai

Yang

Cao

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

In a bioelectrochemical system (BES), microbial community of anode biofilm is crucial to BES performance. In this study, the stratified pattern of community structure and activity of an anode‐respiring biofilm in a BES fueled with brewery wastewater was investigated over time. The anode biofilm exhibited a superior performance in the removal of ethanol to that of an open‐circuit system. The electrical current density reached a high level of 0.55mA/cm2 with a Coulombic efficiency of 71.4%, but decreased to 0.18mA/cm2 in the late stage of operation. A mature biofilm developed a more active outer layer covering a less active inner core, although the activities of the outer and inner layers of biofilm were similar in the early stage. More Geobacter spp., typical exoelectrogens, were enriched in the outer layer than in the inner layer of biofilm in the early stage, while more Geobacter spp. were distributed in the inner layer than in the outer layer in the late stage. The inactive and Geobacter‐occupied inner layer of biofilm might be responsible for the decreased electricity generation from wastewater in the late stage of operation. This study provides better understanding of the effect of anode biofilm structure on BES performance.

show abstract

Protein‐Based Controllable Nanoarchitectonics for Desired Applications

Li,

Zhang,

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Controllable protein nanoarchitectonics refers to the process of manipulating and controlling the assembly of proteins at the nanoscale to achieve domain‐limited and accurate spatial arrangement. In nature, many proteins undergo precise self‐assembly with other structural domains to engage in synergistic physiological activities. Protein nanomaterials prepared through protein nanosizing have received considerable attention due to their excellent biocompatibility, low toxicity, modifiability, and versatility. This review focuses on the fundamental strategies used for controllable protein nanoarchitectinics, which include computational design, self‐assembly induction, template introduction, complexation induction, chemical modification, and in vivo assembly. Precise controlling of the nanosizing process has enabled the creation of protein nanostructures with different dimensions, including 0D spherical oligomers, 1D nanowires, nanorings, and nanotubes, as well as 2D nanofilms, and 3D protein nanocages. The unique biological properties of proteins hold promise for diverse applications of these protein nanomaterials, including in biomedicine, the food industry, agriculture, biosensing, environmental protection, biocatalysis, and artificial light harvesting. Protein nanosizing is a powerful tool for developing biomaterials with advanced structures and functions.

show abstract

Nanocage encapsulation improves antiepileptic efficiency of phenytoin

Cited by 27 publications

References 48 publications

CB1-Antibody Modified Liposomes for Targeted Modulation of Epileptiform Activities Synchronously Detected by Microelectrode Arrays

CB1-Antibody Modified Liposomes for Targeted Modulation of Epileptiform Activities Synchronously Detected by Microelectrode Arrays

Stratified microbial structure and activity within anode biofilm during electrochemically assisted brewery wastewater treatment

Protein‐Based Controllable Nanoarchitectonics for Desired Applications

Contact Info

Product

Resources

About