Nanobiosensors for detection of opioids: A review of latest advancements

Razlansari, Mahtab; Ulucan‐Karnak, Fulden; Kahrizi, Masoud; Mirinejad, Shekoufeh; Sargazi, Saman; Mishra, Sachin; Rahdar, Abbas; Díez‐Pascual, Ana M.

doi:10.1016/j.ejpb.2022.08.017

Cited by 22 publications

(20 citation statements)

References 153 publications

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“…Notably, IGNiTR reports on the general presence of opioids, which complements existing assays for detecting specific synthetic opioid molecules. 44,45 Because the IGNiTR reagents can be readily stored frozen, we envision the components of IGNiTR being packaged into a kit for detecting μ-OR agonists in a variety of settings. Further, IGNiTR can potentially be adapted to detect other GPCR agonists, enabling biosensor development for a wide range of molecules.…”

Section: Resultsmentioning

confidence: 99%

An accessible and generalizable in vitro luminescence assay for detecting GPCR activation

Miller

Sescil

Sarcinella

et al. 2023

Preprint

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G-protein coupled receptors (GPCRs) serve critical physiological roles as the most abundant family of receptors. Here we describe the design of a generalizable and accessibleIn vitroGPCR splitNanoLuc ligandTriggeredReporter (IGNiTR), having broad and diverse applications. IGNiTR leverages the interaction between a conformationspecific binder and agonist-activated GPCR to reconstitute a split nanoluciferase. We have demonstrated IGNiTR with three Gs-coupled GPCRs and a Gi-coupled GPCR with three classes of conformation-specific binders: nanobodies, miniG proteins, and G-protein peptidomimetics. IGNiTR demonstrated binding efficacy and potency values of various Dopamine Receptor D1 (DRD1) ligands that agree well with reported values. IGNiTR also allows the use of a synthetic G protein peptidomimetic, providing easily standardized reagents for characterizing GPCRs and ligands. We demonstrated three applications of IGNiTR: 1) characterizing GPCR functionality during Nanodisc-based reconstitution process; 2) highthroughput screening of ligands against DRD1; 3) detection of opioids for in the field applications. Due to its convenience, accessibility and consistency, IGNiTR will find extensive applications in GPCR ligand detection, screening and GPCR characterization.

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Section: Resultsmentioning

confidence: 99%

An accessible and generalizable in vitro luminescence assay for detecting GPCR activation

Miller

Sescil

Sarcinella

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Our results demonstrate that IGNiTR can successfully detect various levels of opioids. Notably, IGNiTR reports on the general presence of many types of opioids, which complements existing assays for detecting specific synthetic opioid molecules. , Because the IGNiTR reagents can be stored frozen, we envision the components of IGNiTR being packaged into a kit for the detection of MOR agonists in a variety of settings. Furthermore, IGNiTR can potentially be adapted to detect other GPCR agonists, enabling the development of biosensors for a wide range of molecules.…”

Section: Results and Discussionmentioning

confidence: 99%

Accessible and Generalizable in Vitro Luminescence Assay for Detecting GPCR Activation

et al. 2023

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G protein-coupled receptors (GPCRs) serve critical physiological roles as the most abundant family of receptors. Here, we describe the design of a generalizable and cell lysate-based method that leverages the interaction between an agonist-activated GPCR and a conformation-specific binder to reconstitute split nanoluciferase (NanoLuc) in vitro. This tool, In vitro GPCR split NanoLuc ligand Triggered Reporter (IGNiTR), has broad applications. We have demonstrated IGNiTR's use with three G scoupled GPCRs, two G i -coupled GPCRs and three classes of conformation-specific binders: nanobodies, miniG proteins, and G protein peptidomimetics. As an in vitro method, IGNiTR enables the use of synthetic G protein peptidomimetics and provides easily scalable and portable reagents for characterizing GPCRs and ligands. We tested three diverse applications of IGNiTR: (1) proofof-concept GPCR ligand screening using dopamine receptor D1 IGNiTR; (2) detection of opioids for point-of-care testing; and (3) characterizing GPCR functionality during Nanodisc-based reconstitution processes. Due to IGNiTR's unique advantages and the convenience of its cell lysate-based format, this tool will find extensive applications in GPCR ligand detection, screening, and GPCR characterization.

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“…9−15 Laboratories often combine chromatographic methods with immunoassays to ensure both high sensitivity and specificity. 16,17 However, these techniques require specialized equipment and training, making them relatively expensive and time-consuming. Meanwhile, due to the lack of redox activity of norfentanyl, certain electrochemical methods, although are generally considered to be rapid, simple, and low-cost, are not suitable for norfentanyl detection.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Instrumental analytical methods, such as gas chromatography–mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) are the most commonly used techniques for the detection of norfentanyl. − Laboratories often combine chromatographic methods with immunoassays to ensure both high sensitivity and specificity. , However, these techniques require specialized equipment and training, making them relatively expensive and time-consuming. Meanwhile, due to the lack of redox activity of norfentanyl, certain electrochemical methods, although are generally considered to be rapid, simple, and low-cost, are not suitable for norfentanyl detection. , …”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Norfentanyl Sensor Based on a Carbon Nanotube-Based Field-Effect Transistor for the Detection of Fentanyl Exposure

Shao,

Zeng,

Star

2023

ACS Appl. Mater. Interfaces

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The opioid crisis is a worldwide public health crisis that has affected millions of people. In recent years, synthetic opioids, primarily illicit fentanyl, have become the primary driver of overdose deaths. There is a great need for a highly sensitive, portable, and inexpensive analytical tool that can quickly indicate the presence and relative threat of fentanyl. In this work, we develop a semiconductor enriched (sc-) single-walled carbon nanotube (SWCNT)-based field-effect transistor (FET) biosensor functionalized with norfentanyl antibodies for the sensitive detection of norfentanyl, the primary inactive metabolite of fentanyl, in urine samples. Different sensor configurations were explored in order to obtain the most optimized sensing results. Moreover, by employing the "reduced" antibody, we achieved orientated immobilization of the norfentanyl antibody and thus brought the antigen−antibody interaction closer to the sensor surface, further improving the sensitivity. The reported norfentanyl biosensors have a limit of detection in the fg/mL region in both calibration samples and synthetic urine samples, showing ultrasensitivity and high reliability.

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Nanobiosensors for detection of opioids: A review of latest advancements

Cited by 22 publications

References 153 publications

An accessible and generalizable in vitro luminescence assay for detecting GPCR activation

An accessible and generalizable in vitro luminescence assay for detecting GPCR activation

Accessible and Generalizable in Vitro Luminescence Assay for Detecting GPCR Activation

An Ultrasensitive Norfentanyl Sensor Based on a Carbon Nanotube-Based Field-Effect Transistor for the Detection of Fentanyl Exposure

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