Nanobiocomposite Based Electrochemical Sensor for Sensitive Determination of Serotonin in Presence of Dopamine, Ascorbic Acid and Uric Acid In Vitro

Abstract

A multiwalled carbon nanotube – chitosan (MWCNT‐Chit) composite modified glassy carbon electrode (GCE) was fabricated and applied for sensitive and selective determination of serotonin also known as 5‐hydroxytryptamine (5‐HT). Electroanalytical response of the fabricated electrode towards the detection of 5‐HT was studied by using cyclic voltammetry and electrochemical impedance spectroscopy. The results show that the MWCNT‐Chit/GCE has greatly enhanced the oxidation peak current and that the nanocomposite film enhanced the electron transfer to the analyte. Applying differential pulse voltammetry method, a linear calibration plot has been achieved over the concentration range 5×10−8–1.6×10−5 M and the low‐detection‐limit is 5×10−8 M. Selective determination of serotonin in the presence of dopamine, ascorbic acid and uric acid was established. The fabricated biosensor detects 5‐HT in‐vitro directly from artificial urine samples and from diluted serum samples.

Facile Electrochemically Reduced Graphene Oxide‐Multi‐walled Carbon Nanotube Nanocomposite as Sensitive Probe for in‐vitro Determination of Nitrofurantoin in Biological Fluids

“…The low detection limit was determined to be 13.7 nM by considering the slope of the plot and the change in peak current by three relative standard deviations (3σ). Such a parabolic increase in the peak current was reported earlier in several electrochemical sensors [4,19,35]. The parabolic increase in the peak current was observed probably due to a slow heterogeneous electron transfer at the interface.…”

Electrochemical Determination of Serotonin Using Pre‐treated Multi‐walled Carbon Nanotube‐polyaniline Composite Electrode

“…P(EDOP)-SWNTs/GCE DPV 1.0 3 10 À7 -1.0 3 10 À5 5.0 3 10 À9 -[6] MIP-hemin Amperometry 1.0 3 10 À6 -1.0 3 10 À6 1.0 3 10 À3 Serum samples [22] CURC/GCE SWV 1.0 3 10 À7 -1.0 3 10 À5 3.0 3 10 À8 Human blood serum [38] Pt/MWCNT/PPy/AgNPs DPV 5.0 3 10 À7 -5.0 3 10 À6 1.5 3 10 À7 Plasmatic serum sample [16] MWCNT-Chitosan DPV 5.0 3 10 À8 -1.6 3 10 À5 5.0 3 10 À8 Serum samples [39] p(P3CA)/PGE AdDPSV 1.0 3 10 À8 -1.0 3 10 À6 2.5 3 10 À9 Blood serum Urine serum [19] Ion-selective electrode Potentiometry 2. …”

Predicting the Cardiac Troponin I (cTnl) Aptamer/Methylene Blue Configuration Using Computational Modeling Studies: A Screening Search Method for Constructing Aptasensors

“…This confirms the immobilization of the aptamer on the COOH-MWCNT surface. [22,23] CVs for the bare GCE and COOH-MWCNT/GCE before and after the immobilization of the aptamer are recorded in PBS (100 mM pH 7.4) and 100 mM KCl. Curve (b) in Figure 6C shows the existence of a couple of redox waves in the À 0.111 and 0.011 V as cathodic and anodic peak potentials at a scan rate of 0.1 V. s À 1 for COOH-MWCNT/GCE.…”