Nanobacteria May Be Linked to Testicular Microlithiasis in Infertility

Zhang, Qinghua; Lu, Gensheng; Shen, Xiaoxu; Zhou, Zhansong; Fang, Qiang; Zhang, Xin; Li, Longkun; Jin, Xin; Song, Bo

doi:10.2164/jandrol.109.007930

Cited by 21 publications

(28 citation statements)

References 28 publications

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“…39 The same authors hypothesized a feasible role of CNPs in the etiology of testicular microlithiasis and observed CNPs using serological, bacterioscopic, and bacteriological tests in 58.8% (10 out of 17) of semen samples of patients with this disease compared with 5.9% (1 out of 17) of controls. 40 Summing up, it is possible to conclude that CNPs play an important role in the development of the various diseases of the genitourinary tract, particularly affecting the risk and the course of nephrolithiasis and of type III chronic prostatitis/chronic pelvic pain syndrome.…”

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confidence: 99%

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The role of calcifying nanoparticles in biology and medicine

Kutikhin¹,

Брусина²,

Yuzhalin³

2012

IJN

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Calcifying nanoparticles (CNPs) (nanobacteria, nanobacteria-like particles, nanobes) were discovered over 25 years ago; nevertheless, their nature is still obscure. To date, nobody has been successful in credibly determining whether they are the smallest self-replicating life form on Earth, or whether they represent mineralo-protein complexes without any relation to living organisms. Proponents of both theories have a number of arguments in favor of the validity of their hypotheses. However, after epistemological analysis carried out in this review, all arguments used by proponents of the theory about the physicochemical model of CNP formation may be refuted on the basis of the performed investigations, and therefore published data suggest a biological nature of CNPs. The only obstacle to establish CNPs as living organisms is the absence of a fairly accurately sequenced genome at the present time. Moreover, it is clear that CNPs play an important role in etiopathogenesis of many diseases, and this association is independent from their nature. Consequently, emergence of CNPs in an organism is a pathological, not a physiological, process. The classification and new directions of further investigations devoted to the role of CNPs in biology and medicine are proposed.

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confidence: 99%

“…To date, certain studies of this type have been successfully performed (as investigations on animal models 23,26,31,34,38 and as epidemiological studies 16,22,25,32,33,36,37,[39][40][41][42][43][44][45][46][47][48][49] ). Two papers indicate comET-therapy as a possible effective method of treatment of CNP-associated diseases.…”

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confidence: 99%

The role of calcifying nanoparticles in biology and medicine

Kutikhin¹,

Брусина²,

Yuzhalin³

2012

IJN

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“…[39][40][41] Putative nanobacteria have been described using both transmission and scanning electron microscopy, but the immunochemical specificity of these putative nanobacteria has not been established, and DNA staining (Hoechst 33258)…”

Section: Discussionmentioning

confidence: 99%

Association between calcifying nanoparticles and placental calcification

Guo

Zhang²,

Lü³

et al. 2012

IJN

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Background:The purpose of this study was to examine the possible contribution of calcifying nanoparticles to the pathogenesis of placental calcification. Methods: Calcified placental tissues and distal tissue samples were collected from 36 confirmed placental calcification cases. In addition, 20 normal placental tissue samples were obtained as a control group. All the tissue samples were cultured using special nanobacterial culture methods. The cultured calcifying nanoparticles were examined by transmission electron microscopy (TEM), and their growth was monitored by optical density (OD) at a wavelength of 650 nm. 16S rRNA gene expression of the cultured calcifying nanoparticles was also isolated and sequenced. Results: Novel calcifying nanoparticles wrapped with electron-dense shells between 50 nm to 500 nm in diameter were observed in the extracellular matrix of calcified placental tissues. They were detected in placental villi and hydroxyapatite crystals, and contained "nucleic acidlike materials". After isolation and four weeks of culture, 28 of 36 calcified placental tissue samples showed white granular precipitates attached to the bottom of the culture tubes. OD 650 measurements indicated that the precipitates from the calcified placental tissues were able to grow in culture, whereas no such precipitates from the control tissues were observed. The 16S rRNA genes were isolated from the cultured calcifying nanoparticles and calcified placental tissues, and their gene sequencing results implied that calcifying nanoparticles were novel nanobacteria (GenBank JF823648). Conclusion: Our results suggest that these novel calcifying nanoparticles may play a role in placental calcification.

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“…They also noted an improvement of the symptoms after a tetracycline treatment (orally and intravesically). [38] The same authors proposed a theory, in which CNPs had a significant role in the etiology of testicular microlithiasis, [39] implying that CNPs play an important role in the development of genitourinary tract diseases.…”

Section: Calcifying Nanoparticles: Cell Culture Contaminants or Nanobmentioning

confidence: 99%