Nanoarchitectured peroxidase-mimetic nanozymes: mesoporous nanocrystalline α- or γ-iron oxide?

Masud, Mostafa Kamal; Kim, Jeonghun; Billah, M. Motasim; Wood, Kathleen; Shiddiky, Muhammad J. A.; Nguyen, Nam‐Trung; Parsapur, Rajesh Kumar; Kaneti, Yusuf Valentino; Alshehri, Abdulmohsen Ali; Alghamidi, Yousef Gamaan; Alzahrani, Khalid Ahmed; Adharvanachari, Murugulla; Selvam, Parasuraman; Hossain, Md. Shahriar A.; Yamauchi, Yusuke

doi:10.1039/c9tb00989b

Cited by 83 publications

(81 citation statements)

References 49 publications

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“…Catalysis of TMB by natural peroxidases like HRP, as well as peroxidase mimicking nanozymes, in the presence of H 2 O 2 generates a blue colored 1‐electron oxidation product (TMB ox ) which absorbs light at 652 nm and can be used for naked eye evaluation (qualitative) as well as UV‐visible (UV‐vis) quantification of the reaction . Addition of strong acids to the reaction generates the yellow colored diimine terminal oxidation product which absorbs light at 450 nm and is electroactive.…”

Section: Resultsmentioning

confidence: 99%

“…Addition of strong acids to the reaction generates the yellow colored diimine terminal oxidation product which absorbs light at 450 nm and is electroactive. This yellow colored terminal reaction product can be used for UV‐vis (semiquantitative, colorimetric), as well as electrochemical quantification . The process is illustrated in Figure c.…”

Section: Resultsmentioning

confidence: 99%

“…The initial reaction velocity was calculated by Beer‐Lambert Law . The apparent kinetic parameters ( K m and V max ) for both TMB and H 2 O 2 were estimated by using the Michaelis‐Menten and the Lineweaver‐Burk equations as outlined in our previous papers for studying enzyme‐like activity of gold‐loaded nanoporous ferric oxide nanocubes, porous iron oxide nanoflakes, mesoporous nanocrystalline α‐ or γ‐iron oxide, and mesoporous iron oxide …”

Section: Methodsmentioning

confidence: 99%

“…By carefully controlling their geometries, selectivity, catalytic performance, and surface biofunctionalization capabilities of nanozymes can be optimally maneuvered to suit desired applications. Such features have prompted wide ranging research efforts to explore the application of nanozymes in areas as diverse as industrial production and pollutant removal as well as disease therapies and biosensing . Previous research works have found that various metallic nanoparticles (e. g., gold, platinum, palladium, etc.…”

Section: Introductionmentioning

confidence: 99%

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Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing

et al. 2020

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This article reports on the peroxidase-like catalytic activity of polyoxometalates (POMs) and their potential use as natural peroxidases for developing a simple and efficient colorimetric glucose sensor. Two Keggin-type vanadium-substituted tungstosulfates, [SVW 11 O 40 ] 3À (SVW 11) and [SV 2 W 10 O 40 ] 4À (SV 2 W 10), were tested for their potential as natural enzyme mimetics and exhibited strong peroxidase-like catalytic activity. The catalysis reaction was found to be in accordance with Michaelis-Menten and Lineweaver-Burk kinetics models. Michaelis-Menten constant (K m) and maximum velocity (V max) parameters were calculated to be 0.0759 mM and 0.329 × 10 À 8 Ms À 1 for SVW 11 , and 0.0543 mM and 2.67 × 10 À 8 Ms À 1 for SV 2 W 10 , respectively, indicating a high catalytic activity and a strong affinity of POMs towards 3,3,5,5-tetramethylbenzidine (TMB). In the case of H 2 O 2 , these values were found to be 57.1 mM and 0.325 × 10 À 8 mMs À 1 for SVW 11 , and 47.7 mM and 2.72 × 10 À 8 mMs À 1 for SV 2 W 10. The peroxidase-like catalytic activity of these POMs was used to develop colorimetric glucose sensors as a proof-of-concept model for the POM-based naked-eye detection of biomolecules. The limit of detcetion (LOD) of glucose for SVW 11 and SV 2 W 10 was 1.14 μM and 1.24 μM, respectively. Our findings propose broad-ranging potential applications of these novel POMs in biosensing and bioanalytical chemistry.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing

et al. 2020

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“…Various techniques have been developed for the H 2 O 2 detection including the colorimetric assay [ 3 , 4 ], fluorescence detection [ 5 , 6 ], electrochemical luminescence [ 7 , 8 ], surface-enhanced Raman spectroscopy (SERS) [ 9 , 10 ] etc. Among these, electrochemical sensing [ 11 ] can offer one of the handiest approaches for the detection of H 2 O 2 due to its high sensitivity, fast response, precision and simple operation.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen Peroxide Detection by Super-Porous Hybrid CuO/Pt NP Platform: Improved Sensitivity and Selectivity

et al. 2020

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A super-porous hybrid platform can offer significantly increased number of reaction sites for the analytes and thus can offer advantages in the biosensor applications. In this work, a significantly improved sensitivity and selectivity of hydrogen peroxide (H2O2) detection is demonstrated by a super-porous hybrid CuO/Pt nanoparticle (NP) platform on Si substrate as the first demonstration. The super-porous hybrid platform is fabricated by a physiochemical approach combining the physical vapor deposition of Pt NPs and electrochemical deposition of super-porous CuO structures by adopting a dynamic hydrogen bubble technique. Under an optimized condition, the hybrid CuO/Pt biosensor demonstrates a very high sensitivity of 2,205 µA/mM∙cm2 and a low limit of detection (LOD) of 140 nM with a wide detection range of H2O2. This is meaningfully improved performance as compared to the previously reported CuO-based H2O2 sensors as well as to the other metal oxide-based H2O2 sensors. The hybrid CuO/Pt platform exhibits an excellent selectivity against other interfering molecules such as glucose, fructose, dopamine, sodium chloride and ascorbic acid. Due to the synergetic effect of highly porous CuO structures and underlying Pt NPs, the CuO/Pt architecture offers extremely abundant active sites for the H2O2 reduction and electron transfer pathways.

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Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

Feng,

Wang,

Wang

et al. 2024

Advanced Materials

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Although nanozymes have drawn a great attention over the past decade, the activities of peroxidase‐like, oxidase‐like and catalase‐like nanozymes are often pH dependent with elusive mechanism, which largely restricts their application. Therefore, a systematical discussion on the pH related catalytic mechanisms of nanozymes together with the methods to overcome this limitation is in need. In this review, various nanozymes exhibiting pH dependent catalytic activities are collected and the root causes for their pH dependence are comprehensively analyzed. Subsequently, regulatory concepts including catalytic environment reconstruction and direct catalytic activity improvement to break this pH restriction are summarized. Moreover, applications of pH independent nanozymes in sensing, disease therapy and pollutant degradation are overviewed. Finally, current challenges and future opportunities on the development of pH independent nanozymes are suggested. It is anticipated that this review will promote the further design of pH independent nanozymes and broaden their application range with higher efficiency.This article is protected by copyright. All rights reserved

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Nanoarchitectured peroxidase-mimetic nanozymes: mesoporous nanocrystalline α- or γ-iron oxide?

Abstract: Next-generation nanozyme based biosensing: mesoporous nanocrystalline α- or γ-iron oxide?

Cited by 83 publications

References 49 publications

Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing

Vanadium‐Substituted Tungstosulfate Polyoxometalates as Peroxidase Mimetics and Their Potential Application in Biosensing

Hydrogen Peroxide Detection by Super-Porous Hybrid CuO/Pt NP Platform: Improved Sensitivity and Selectivity

Breaking the pH Limitation of Nanozymes: Mechanisms, Methods, and Applications

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