Nanoantennas as Biomarkers for Bacterial Detection

Abstract

Understanding the biology of bacteria is critical for exploiting their beneficial properties and for preventing and treating bacterial diseases. Nanobioscience is an area that has recently seen major scientific progress. Here, we demonstrate that a raspberry-shaped nanostructure with a high density of gold nanoparticles acts like an excellent antenna due to its optical properties, which permit sensitive detection and analysis of bacterial cells. By using antibodies, these nanoantennas can be engineered to recognize only specific bacterial species. This system provides a new technique that will allow for more sensitive detection of specific bacteria.

Light-scattering Characteristics of Metal Nanoparticles on a Single Bacterial Cell

“…16 Gram-negative bacteria possess a negatively charged surface (-40 --20 mV) when dispersed in a wide range of pH. 17,18 This suggests that the negative charge is pre-dominant on the surface of a bacterium due to phosphate and carboxylate groups compared with amino groups which are included in LPS. 19 To achieve accurate cell imaging, it is necessary to design the NPs in terms of the sensitivity and selectivity.…”

“…Recently, we succeeded in visualizing the antigen on the surface of E. coli O157 by using the Au NPs, which have the anti-O157 antibody as a specific recognition site. 17 Therefore, it is possible that the selectivity is easily accomplished by introducing bio-probes, such as antibodies, enzymes and proteins into the NPs. In the present work, we examined the light-scattering characteristics of metal NPs, such as silver (Ag), Au, and copper oxide (Cu2O).…”

Optical Characterization of Gold Nanoparticle Layers Formed on Plastic Microbeads

“…5 Moreover, the NPs can be used as building blocks for fabricating micrometer-sized structures, such as dots, wires, and films. [6][7][8][9][10][11][12] Self-assembly technology can be effectively used to assemble well-organized one-to threedimensional microstructures, and inter-particle connections can be controlled at the single-particle level. 13 Typically, these zeroto two-dimensional microstructures are assessed by using highresolution microscopies, such as scanning probe microscopy (SPM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).…”

Electrochemical Response of Acridine Orange in Bacterial Cell

“…Simple detection methods for pathogenic bacteria in food, clinical, and environmental samples are required to prevent and diagnose these diseases. [1][2][3][4][5] On the other hand, bacteria play various kinds of essential roles in the ecological system. For example, degrading bacteria that convert organic materials to their inorganic forms, which provide nutrition to plants, is essential for soil fertility.…”