Nano-Vesicles of Salbutamol Sulphate in Metered Dose Inhalers: Formulation, Characterization and in Vitro Evaluation

Arafa, Mona G.; Ayoub, Bassam M.

doi:10.22159/ijap.2017v9i6.22448

Cited by 20 publications

(10 citation statements)

References 25 publications

(26 reference statements)

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“…AT-PLGA-CS-NP morphology was inspected by transmission electron microscopy (TEM; H-600, Hitachi, Tokyo, Japan). 20,21 Entrapment Efficiency (EE) and Drug Loading of AT-PLGA-CN-NPs…”

Section: Preparation Of At-plga-cs-npsmentioning

confidence: 99%

“…Zero-order, first-order, and Higuchi diffusion models of linear regression and the Korsmeyer-Peppas model were used to study the in vitro release of AT. 20 In vivo Anti-Inflammatory Assessment of AT-PLGA-CS-NP Thermosensitive Gels Albino rabbits (2-2.5 kg) were obtained from Egyptian farms and kept under standard laboratory conditions. All animal work was conducted in accordance with the guidelines for the care and use of laboratory animals and was approved by the ethical committee of faculty of pharmacy of the British University in Egypt.…”

Section: Kinetic Studymentioning

confidence: 99%

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<p>Chitosan-Coated PLGA Nanoparticles for Enhanced Ocular Anti-Inflammatory Efficacy of Atorvastatin Calcium</p>

Arafa¹,

Girgis²,

El-Dahan³

2020

IJN

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Background: Atorvastatin calcium (AT) is an ocular anti-inflammatory with limited bioavailability when taken orally due to its low solubility in low pH and extensive first-pass effect. To overcome these problems, AT was entrapped in polymeric nanoparticles (NPs) to improve surface properties and sustained release, in addition to achieving site-specific action. Methods: AT was entrapped in chitosan (CS)-coated polylactic-co-glycolic acid (PLGA) NPs to form AT-PLGA-CS-NPs (F1). F1 and free AT were embedded in thermosensitive Pluronic ® 127-hydroxypropyl methylcellulose (HPMC) to form thermosensitive gels (F2) and (F3) while F4 is AT suspension in water. F1 was assessed for size, surface charge, polydispersity index (PDI), and morphology. F2 and F3 were examined for gelation temperature, gel strength, pH, and viscosity. In vitro release of the four formulations was also investigated. The ocular irritancy and anti-inflammatory efficacy of formulations against prostaglandin E 1-(PGE 1) induced ocular inflammation in rabbits were investigated by counting the polymorphonuclear leukocytes (PMNs) and protein migrated in tears. Results: Oval F1 of 80.0-190.0±21.6 nm exhibited a PDI of 0.331 and zeta potential of 17.4 ±5.62 mV with a positive surface charge. F2 and F3 gelation temperatures were 35.17±0.22°C and 36.93±0.31°C, viscosity 12,243±0.64 and 9759±0.22 cP, gel strength 15.56±0.6 and 12.45 ±0.1 s, and pHs of 7.4±0.02 and 7.4±0.1, respectively. In vitro release of F1, F2, F3, and F4 were 48.21±0.31, 26.48±0.5, 84.76±0.11, and 100% after 24 hrs, respectively. All formulations were non-irritant. F2 significantly inhibited lid closure up to 3 h, PMN counts and proteins in tear fluids up to 5 h compared to other formulations. Conclusion: AT-PLGA-CS-NP thermosensitive gels proved to be successful ocular antiinflammatory drug delivery systems.

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“…AT-PLGA-CS-NP morphology was inspected by transmission electron microscopy (TEM; H-600, Hitachi, Tokyo, Japan). 20,21 Entrapment Efficiency (EE) and Drug Loading of AT-PLGA-CN-NPs…”

Section: Preparation Of At-plga-cs-npsmentioning

confidence: 99%

Section: Kinetic Studymentioning

confidence: 99%

<p>Chitosan-Coated PLGA Nanoparticles for Enhanced Ocular Anti-Inflammatory Efficacy of Atorvastatin Calcium</p>

Arafa¹,

Girgis²,

El-Dahan³

2020

IJN

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“…Dissolution test was performed on three Marizev® tablets by dissolution USP procedure using 900 ml of 0.1 N HCl. Mild agitation of 50 rpm was enough to study the release of the drug based on withdrawn aliquots at predetermined intervals for 60 min . Furthermore, 5 ml sample was withdrawn through a 0.45 μm syringe filter and replaced with 5 ml of 0.1 N HCl at 5, 10, 15, 20, 25, 30, 40, 50 and 60 min and then the procedure mentioned earlier was repeated for measuring the previously mentioned withdrawn 5 ml samples after dilution in 25 ml volumetric flasks using water.…”

Section: Methodsmentioning

confidence: 99%

Factorial design optimization of micelle enhanced synchronous spectrofluorimetric assay of Omarigliptin: Applied to content uniformity testing and in vitro drug release

2018

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A micelle enhanced spectrofluorimetric method was developed for determination of Omarigliptin (OMG) based on its native fluorescence behavior. The interaction of OMG with surfactants and macromolecules was studied. In aqueous solution, the relative fluorescence intensity (RFI) of OMG was enhanced by 24% in the presence of Tween 80 at pH 3.5. The optimal conditions for the micelle enhanced fluorescence were attained by Minitab® program using Plackett-Burman factorial design. Pareto chart, contour plots and surface plots were used to exclude the insignificant variables and optimize the significant factors. The spectrofluorimeter was operated under synchronous mode using ∆λ = 30 nm and recording the RFI of the intense narrow band at 267 nm for OMG in 0.5% w/v Tween 80 + 0.2 M acetate buffer (pH 3.5) system using water as diluent. Using synchronous scan mode offered many advantages including considerable reduction of spectral overlap and enhanced linearity of the calibrators. Validation parameters were satisfied over the concentration range 0.1-2 μg/ml. The developed method was the first analytical procedure for OMG assay in Marizev® tablets. Moreover, content uniformity testing and in vitro drug release of tablets were performed.

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“…Our results were in good agreement with Arafa et al, [2017] who found that the release profile of salbutamol sulphate from niosomes followed Higuchi model. This kinetic pattern indicated that the drug release was dominated by diffusion model which normally depended on drug concentration gradient between nano-vesicles and dissolution media with penetration of this media through a porous wall which accompanied by matrix disruption [21]. …”

Section: Entrapment Efficiency Of Itraconazole Proniosomesmentioning

confidence: 99%

“…This was due to the acyl-chain structure of span 60, which could affect cholesterol interactions causing variations in cholesterol distribution. The polar head group of non-ionic surfactant must cover the non-polar portion of cholesterol; this coverage is essential to avoid the unfavourable free energy of cholesterol that when contacts with water decrease the repulsion between cholesterol molecules [21].…”

Section: Optimization Of the Formulation Ingredientsmentioning

confidence: 99%

Formulation and Optimization of Itraconazole Proniosomes Using Box Behnken Design

et al. 2018

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Objective: The aim of the present study was to obtain an optimized formula of itraconazole (ITC) proniosomes using Box Behnken design.Methods: Itraconazole proniosomes were prepared using span 60 and/or brij 35 as surfactants, cholesterol and lecithin as a penetration enhancer by slurry method. Various trials have been carried out for investigation of proniosomes. Parameters such as entrapment efficiency (EE%), in vitro drug release, zeta potential, vesicle size and Transmission Electron Microscope were assessed for evaluation of proniosomes.Results: Entrapment efficiency (EE%) was found to be between 78.56% and 95.46%. The release profile of itraconazole proniosomes occurred in two distinct phases, an initial phase for about 8 h, followed by a slow phase for 16 h. The release pattern shown by these formulations was Higuchi diffusion controlled mechanism. The zeta potential values for all itraconazole proniosomes were in the range of-21.71 to-34.53 mV which confirms their stability. All itraconazoleproniosomes formula was found to be nano-sized and were appeared to be spherical in shape with sharp boundaries. One way analysis of variance (ANOVA) study showed that HLB (X1) had the main effects on most responses (Y). Conclusion:Box behnken design facilitates optimization of the formulation ingredients on entrapment efficiency, in vitro release of itraconazole proniosomes, zeta potential and vesicle size. Finally, an optimum level of factors was provided by the optimization process.

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Nano-Vesicles of Salbutamol Sulphate in Metered Dose Inhalers: Formulation, Characterization and in Vitro Evaluation

Cited by 20 publications

References 25 publications

<p>Chitosan-Coated PLGA Nanoparticles for Enhanced Ocular Anti-Inflammatory Efficacy of Atorvastatin Calcium</p>

<p>Chitosan-Coated PLGA Nanoparticles for Enhanced Ocular Anti-Inflammatory Efficacy of Atorvastatin Calcium</p>

Factorial design optimization of micelle enhanced synchronous spectrofluorimetric assay of Omarigliptin: Applied to content uniformity testing and in vitro drug release

Formulation and Optimization of Itraconazole Proniosomes Using Box Behnken Design

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