Nano-peroxidase fabrication on cation exchanger nanocomposite: Augmenting catalytic efficiency and stability for the decolorization and detoxification of Methyl Violet 6B dye

Current status and emerging frontiers in enzyme engineering: An industrial perspective

“…The dyes produced by these corporations have the ability to alter both the cloudiness and purity of water, while also posing a risk of causing cancer and toxicity to plants and animals. Multiple experiments conducted by Ali et al demonstrate significant enhancements in enzyme decontamination through the use of various methods involving immobilized enzymes [ 169 ]. In a particular study, ginger peroxidase (GP) was immobilized through adsorption using a nanocomposite material consisting of polypyrrole-cellulose-graphene oxide [ 33 ].…”

“…GP was immobilized through electrostatic interaction with a nanocomposite of polypyrrole-zirconium (IV) selenoiodate cation exchanger. This immobilization resulted in enhanced stability (both pH and temperature), improved decolorization efficiency of Methyl Violet 6B Dye (96 % compared to 88 % for free GP), and a tenfold increase in maximal velocity [ 169 ]. Both systems employ nanoparticles, which offer numerous benefits due to their extensive surface area, efficient enzyme loading, and strong mechanical properties.…”

Enzyme engineering and its industrial applications

“…The dyes released by these companies can not only change the water turbidity and transparency but also are carcinogenic and toxic to animals and plants. Several studies from Ali et al (85) show great improve in enzyme decontamination using different approaches with immobilized enzymes. In one of those papers (84) ginger peroxidase (GP) was immobilized by adsorption mechanism with polypyrrole-cellulose-graphene oxide nanocomposite.…”

“…The result was an improved decolorization efficiency of Reactive Blue 4 Dye (RB4) (11% higher than the free GP), maximal velocity (3.3× higher) and affinity to RB4. GP was also immobilized by electrostatic interaction with polypyrrole-zirconium (IV) selenoiodate cation exchanger nanocomposite and was enhanced stability (pH and temperature), decolorization efficiency of Methyl Violet 6B Dye (96% against 88% of free GP) and the maximal velocity was ten times higher (85). Both strategies use nanosized particles, which have many advantages because of their large surface area, effective enzyme loading, and high mechanical strength.…”

“…Several studies from Ali et al. (85) show great improve in enzyme decontamination using different approaches with immobilized enzymes. In one of those papers (84) ginger peroxidase (GP) was immobilized by adsorption mechanism with polypyrrole‐cellulose‐graphene oxide nanocomposite.…”