2019
DOI: 10.1038/s41598-019-48566-7
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Nano-mechanical mapping of interdependent cell and ECM mechanics by AFM force spectroscopy

Abstract: Extracellular matrix (ECM), as a dynamic component of the tissue, influences cell behavior and plays an important role in cell mechanics and tissue homeostasis. Reciprocally, this three-dimensional scaffold is dynamically, structurally and mechanically modified by cells. In the field of biophysics, the independent role of cell and ECM mechanics has been largely investigated; however, there is a lack of experimental data reporting the interdependent interplay between cell and ECM mechanics, measured simultaneou… Show more

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Cited by 60 publications
(42 citation statements)
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“…More analysis is required in order to get a deeper understanding of the scaffold’s topographical properties and how these might affect seeded endothelial cells. One method would be to use technologies such as atomic force microscopy (AFM), which have previously been used to study the interaction of cells with their scaffold/substrate [ 64 , 65 ]. Furthermore, the infiltration depth of cells could have an impact on the noted gene expression.…”
Section: Discussionmentioning
confidence: 99%
“…More analysis is required in order to get a deeper understanding of the scaffold’s topographical properties and how these might affect seeded endothelial cells. One method would be to use technologies such as atomic force microscopy (AFM), which have previously been used to study the interaction of cells with their scaffold/substrate [ 64 , 65 ]. Furthermore, the infiltration depth of cells could have an impact on the noted gene expression.…”
Section: Discussionmentioning
confidence: 99%
“…In vivo , mechanical stress is transduced into the cell from the sites at which the cells attach to the ECM. The cells may engage their ECM both via mechanosensitive adhesion complexes and via other surface receptors, including those for growth factors and inflammatory mediators, which cannot act as adhesive anchors but may modify the mechanical signals transduced at the cell/ECM interface ( 79 ). Under these conditions, activated mechanosensitive plasma membrane channels allow the inflow of Ca 2+ that can act as a second messenger to regulate gene expression.…”
Section: Introductionmentioning
confidence: 99%
“…To initiate and control this transition, temperature gradients, chemical or photochemical crosslinking or shear thinning are used [3,5,23,24], and technologies which allow to monitor this transition can help to optimize printing parameters and are required for quality control [25,26]. Currently, indentation type atomic force microscopy (IT-AFM) is used as a standard method for spatially resolved characterization of the biomechanical properties of biological samples, such as cells, ECM, hydrogels and tissue sections with high spatial resolution [8,21,[27][28][29][30]. However, IT-AFM is comparatively slow, it relies on mechanical contact between an indenter (typically a pyramidal tip) and the biological sample and is not readily integrated into biofabrication or other fabrication processes.…”
Section: Introductionmentioning
confidence: 99%