Nano-materials for Gene Therapy: An Efficient Way in Overcoming Challenges of Gene Delivery

Massadeh, Salam; Alaamery, Manal; Bawazeer, Shahad; AlAhmad, Othman; AlSubai, Rawan; Barker, Suzan; Craig, Duncan Q.M.

doi:10.4172/2155-6210.1000195

Cited by 27 publications

(8 citation statements)

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“…Great efforts have been made to understand how dsRNAs, siRNAs, and microRNAs (miRNAs) are able to affect control expression in eukaryotic cells. As described previously, siRNA technology mediates specifically gene silencing at different levels like mRNA degradation, chromatin modification, and translational repression 60–64. Remarkable findings were also found and confirmed transcriptional gene silencing was conserved in mammalian cells when were mediated by siRNAs 65.…”

Section: Sirna-based Gene Silencingsupporting

confidence: 77%

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Chalbatani¹,

Dana²,

Gharagouzloo³

et al. 2019

IJN

178

107

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Cancer is one of the most complex diseases that has resulted in multiple genetic disorders and cellular abnormalities. Globally, cancer is the most common health concern disease that is affecting human beings. Great efforts have been made over the past decades in biology with the aim of searching novel and more efficient tools in therapy. Thus, small interfering RNAs (siRNAs) have been considered one of the most noteworthy developments which are able to regulate gene expression following a process known as RNA interference (RNAi). RNAi is a post-transcriptional mechanism that involves the inhibition of gene expression through promoting cleavage on a specific area of a target messenger RNA (mRNA). This technology has shown promising therapeutic results for a good number of diseases, especially in cancer. However, siRNA therapeutics have to face important drawbacks in therapy including stability and successful siRNA delivery in vivo. In this regard, the development of effective siRNA delivery systems has helped addressing these issues by opening novel therapeutic windows which have allowed to build up important advances in Nanomedicine. In this review, we discuss the progress of siRNA therapy as well as its medical application via nanoparticle-mediated delivery for cancer treatment.

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Section: Sirna-based Gene Silencingsupporting

confidence: 77%

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Chalbatani¹,

Dana²,

Gharagouzloo³

et al. 2019

IJN

178

107

View full text Add to dashboard Cite

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“…Although, many efforts have been carried out in this field, gene therapy is still in its early stages of development and even it has been accompanied with defeats in the past. Identification of therapeutic gene and its efficient delivery into the nucleus of the desired cells along with its tracking are key factors in a successful gene therapy process 3 – 5 . A suitable nano-carrier for gene therapy should have high efficiency, no cytotoxicity and immunogenicity, capable of carrying gene and protecting it against degradation, overcome biological barriers and gene delivery 6 – 10 .…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Gene Delivery and Tracking through Preparation of Photo-Luminescent Nanoparticles Based on Graphene Quantum Dots and Chimeric Peptides

Ghafary

Nikkhah

Hatamie

et al. 2017

Sci Rep

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Designing suitable nano-carriers for simultaneous gene delivery and tracking is in the research priorities of the molecular medicine. Non-toxic graphene quantum dots (GQDs) with two different (green and red) emission colors are synthesized by Hummer’s method and characterized by UV-Vis, Photoluminescence (PL), Fourier Transform Infrared (FTIR) and Raman spectroscopies, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The GQDs are conjugated with MPG-2H1 chimeric peptide and plasmid DNA (pDNA) by non-covalent interactions. Following conjugation, the average diameter of the prepared GQDs increased from 80 nm to 280 nm in complex structure, and the ζ-potential of the complex increased (from −36.87 to −2.56 mV). High transfection efficiency of the nano-carrier and results of confocal microscopy demonstrated that our construct can be considered as a nontoxic carrier with dual functions for gene delivery and nuclear targeting.

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“…Gene therapy has grown colossal interest for researchers due to its ability to replace a gene of interest with a healthy gene and can be very useful for surgery and treating drugs. For locating the desired gene location in an input genomic sequence, the proposed LLAD based AEPs with low computational complexities can be used in Nano devices [8].…”

Section: Table 1 Multiplication Operations Required For Implementation Of Various Logarithmic Aepsmentioning

confidence: 99%

“…To produce beneficial tools at Nano measure using biological blocks, bio-nanotechnology science is used [6], [7]. To treat or prevent diseases, gene therapy is used to directly insert required gene into a specific cell after identifying the gene of interest [8]. For locating the desired gene location in an input genomic sequence, the proposed LLAD based AEPs with low computational complexities can be used in Nano devices.…”

Section: Introductionmentioning

confidence: 99%

New Exon Prediction Techniques Using Adaptive Signal Processing Algorithms for Genomic Analysis

2019

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Substantial research and monetary aids to healthcare establishments are provided by cloud computing. A benign position to store and handle vast genome data is offered by cloud services. Labs for gene sequencing send out raw and contingent data over the Internet to multiple sequence collections under conservative flow of gene information. The use of cloud services also reduces the storage costs of deoxyribonucleic acid (DNA) sequencing. Here, an efficient and new bio-informatics genomic system is proposed by the use of cloud services from Amazon to access the stored gene data and process it. A key task in bio-informatics is to locate protein-coding sections in a gene sequence based on three base periodicity (TBP) is for disease diagnosis and design drugs. Here, a novel cloud-based adaptive exon predictor (AEP) using Amazon cloud services is proposed to improve the accuracy in exon finding ability as well as aimed at superior convergence. Noise in the input gene sequence given to the proposed AEPs is pre-processed using normalized LMS filtering. Computational complexity can be reduced using proposed data normalized form of least logarithmic absolute difference (NLLAD) algorithm and its error normalized variants. It was shown that sign regressor NLLAD (SRNLLAD) dependent AEP is efficient in exon forecast applications using different metrics for a performance like sensitivity 0.8037, precision 0.8052 along with specificity 0.8146 by different gene sequences considered from the National Center for Biotechnology Information (NCBI) databank. The proposed AEPs have shown upright performance than typical LMS and other AEPs in terms of exon prediction accuracy, convergence, and computational complexity. Their less computational complexity will be found attractive, and they are suitable to use in bio-informatics nano devices.INDEX TERMS Amazon cloud services, bio-informatics, convergence, deoxyribonucleic acid, National Center for Biotechnology Information, base three periodicity.

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Nano-materials for Gene Therapy: An Efficient Way in Overcoming Challenges of Gene Delivery

Cited by 27 publications

References 0 publications

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

<p>Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach</p>

Simultaneous Gene Delivery and Tracking through Preparation of Photo-Luminescent Nanoparticles Based on Graphene Quantum Dots and Chimeric Peptides

New Exon Prediction Techniques Using Adaptive Signal Processing Algorithms for Genomic Analysis

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