Nano hemostat solution: immediate hemostasis at the nanoscale

Abstract

Hemostasis is a major problem in surgical procedures and after major trauma. There are few effective methods to stop bleeding without causing secondary damage. We used a self-assembling peptide that establishes a nanofiber barrier to achieve complete hemostasis immediately when applied directly to a wound in the brain, spinal cord, femoral artery, liver, or skin of mammals. This novel therapy stops bleeding without the use of pressure, cauterization, vasoconstriction, coagulation, or cross-linked adhesives. The self-assembling solution is nontoxic and nonimmunogenic, and the breakdown products are amino acids, which are tissue building blocks that can be used to repair the site of injury. Here we report the first use of nanotechnology to achieve complete hemostasis in less than 15 seconds, which could fundamentally change how much blood is needed during surgery of the future.

Novel Infectious Agent-Free Hemostatic Material (TDM-621) in Cardiovascular Surgery

“…This peptide hydrogel covers the bleeding point to physically occlude the vascular adventitia of the broken vessel and cause blood coagulation in the vascular intima, resulting in hemostasis. The efficacy of hemostasis using these properties was first demonstrated in an animal experiment by Ellis-Behnke, et al 1) We have undertaken the clinical development of TDM-621 as an absorbent topical hemostatic material, while focusing on its physicochemical properties and biocompatibility. To evaluate the hemostatic efficacy of TDM-621, we designed the present study based on the results of an efficacy validation study of an approved hemostatic material, and constructed a rabbit abdominal aortic puncture bleeding model and a beagle abdominal aortic graft replacement model simulating clinical use.…”

Self-Assembled Peptides: Characterisation and In Vivo Response

“…Each of these systems has associated advantages and disadvantages, providing stimulus for continuing research investigating the use of SAP scaffolds for this purpose. The space-filling properties of an SAP scaffold that can assemble in vivo have been shown to provide haemostatic properties [70]. The injection of a solution of RADA based peptides resulted in the formation of an integrated nanofibrous barrier that quickly and effectively stopped bleeding in a range of tissue types including liver, brain, spinal cord, artery and skin [71].…”

Nanotechnology for Surgeons

“…Non-composite nanomaterials and nanotechnological approaches cover a wide range of surgical topics, including self-assembling nanofibres for haemostasis [37], nanofabricated drains [38], conduits for nerve repair [39] and means of studying and affecting in vivo processes such as neuroprotection and neuromodulation in real time [40].…”