Nano-fluorescent probes based on DNA-templated copper nanoclusters for fast sensing of thiocyanate

Tao, Ling; Huang, Ting; Wang, Tianze; Yu, Ping; Qing, Taiping; Nie, Beixi

doi:10.1039/d0nj03742g

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…The results shown in Figure 3C demonstrated that the freshly prepared CuNCs exhibited a uniform spherical shape with a diameter of 5 nm, which was consistent with our previous work. 37 CuNCs could be easily oxidized by gaseous or dissolved oxygen, resulting in their low catalytic activity and poor stability. 38 Therefore, it was necessary to investigate the nanostructure stability of CuNCs.…”

Section: Characterization and Stability Of Dna-templated Cuncsmentioning

confidence: 99%

Sensitive Detection of BRCA1 Gene Based on Target Proximity Induced Quenching of the Fluorescence of Copper Nanoclusters

Hu¹,

Yang²,

Li³

et al. 2023

J. Braz. Chem. Soc.

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The mutations of breast cancer susceptibility gene 1 (BRCA1) play an important role in inherited breast cancers. Thus, the sensitive assay of the BRCA1 gene is extremely important for disease diagnosis and human health. Herein, a fast, sensitive and selective assay technique has been constructed based on fluorescent copper nanoclusters (CuNCs). The CuNCs were successfully formed with poly(AT-TA) double stranded DNA (dsDNA) as template. In the absence of the target, a strong red emission was observed under 365 nm ultraviolet (UV) lamp and a big fluorescence response was obtained. However, in the presence of BRCA1 gene, there was only weak red emission and a low response signal was produced because of the target-proximity induced quenching of the fluorescence of CuNCs. The linear range for the BRCA1 gene assay was 2-600 nM, and the limit of detection was 2 nM. The assay technique showed good selectivity, good stability and satisfactory recoveries for the detection of BRCA1 gene in diluted serum samples. Moreover, by integrating a UV lamp and a smartphone, the fluorescence sensor would be transferred to a microfluidic chip, providing a prospective application in point-of-care for monitoring breast cancer risk.

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Section: Characterization and Stability Of Dna-templated Cuncsmentioning

confidence: 99%

Sensitive Detection of BRCA1 Gene Based on Target Proximity Induced Quenching of the Fluorescence of Copper Nanoclusters

Hu¹,

Yang²,

Li³

et al. 2023

J. Braz. Chem. Soc.

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“…The invented fluorescence sensor can detect SCN − with high sensitivity of 4.94 mM in the linear range of 10–100 mM. [ 178 ]…”

Section: Functional Nanomaterials In Assaying Biomarkersmentioning

confidence: 99%

“…The invented fluorescence sensor can detect SCN À with high sensitivity of 4.94 mM in the linear range of 10-100 mM. [178] A reliable inverse micelle approach was used to design highly When fluorescein is bound to the surface of AuNPs, its fluorescence is significantly inhibited. The addition of SCN À turned on the fluorescence due to the competing activity of fluorescein and SCN À towards the surface of AuNPs.…”

Section: Inorganic Species As Biomarkersmentioning

confidence: 99%

Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

Harshita

Kailasa

2022

Luminescence

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This review briefly emphasizes the different detection approaches (electrochemical sensors, chemiluminescence, surface‐enhanced Raman scattering), functional nanostructure materials (quantum dots, metal nanoparticles, metal nanoclusters, magnetic nanomaterials, metal oxide nanoparticles, polymer‐based nanomaterials, and carbonaceous nanomaterials) and detection mechanisms. Furthermore, the emphasis of this review is on the integration of functional nanomaterials with optical spectroscopic techniques for the identification of various biomarkers (nucleic acids, glucose, uric acid, oxytocin, dopamine, ascorbic acid, bilirubin, spermine, serotonin, thiocyanate, Pb2+, Cu2+, Hg2+, F−, peptides), and cancer biomarkers (mucin 1, prostate specific antigen, carcinoembryonic antigen, CA15‐3, human epidermal growth factor receptor 2, C‐reactive protein, and interleukin‐6). Analytical characteristics of nanomaterials‐based optical sensors are summarized in the tables, providing the insights of nanomaterials‐based optical sensors for biomarkers detection. Finally, the opportunities and challenges of nanomaterials‐based optical analytical approaches for the detection of various biomarkers (inorganic, organic, biomolecules, peptides and proteins) are discussed.

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“AND”-Logic gate-based colorimetric detection of thiocyanate in milk samples using AgNP-EBF as plasmonic nano sensor

Borah,

Kaka,

Tamuly

2023

Food Chemistry

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Nano-fluorescent probes based on DNA-templated copper nanoclusters for fast sensing of thiocyanate

Abstract: Thiocyanate (SCN-) is a metabolite of cyanide detoxification, which is considered as a biomarker of environmental tobacco smoke exposure. Herein, a simple, fast and label-free fluorescent sensor was developed to...

Cited by 6 publications

References 41 publications

Sensitive Detection of BRCA1 Gene Based on Target Proximity Induced Quenching of the Fluorescence of Copper Nanoclusters

Sensitive Detection of BRCA1 Gene Based on Target Proximity Induced Quenching of the Fluorescence of Copper Nanoclusters

Recent advances in nanomaterials‐based optical sensors for detection of various biomarkers (inorganic species, organic and biomolecules)

“AND”-Logic gate-based colorimetric detection of thiocyanate in milk samples using AgNP-EBF as plasmonic nano sensor

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