Nano-evolution and protein-based enzymatic evolution predicts novel types of natural product nanozymes of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (<i>Rhizoma polygonati</i>) and Goji (<i>Lycium chinense</i>)

Nazarbek, Guldan; Кутжанова, А. Н.; Nurtay, Lazzat; Mu, Chenglin; Kazybay, Bexultan; Li, Xugang; Ma, Cuiping; Amin, Amr; Xie, Yingqiu

doi:10.1039/d1na00475a

Cited by 16 publications

(5 citation statements)

References 29 publications

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“…Moreover, P. ginseng significantly elevated the GP serum level. Several studies (Al-Dabbagh et al, 2018;Li et al, 2020;Nazarbek et al, 2021) confirmed this finding. As well, the MDA serum level showed a significant reduction after the administration of P. ginseng, coinciding with a previous report (Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 64%

Combination of Panax ginseng C. A. Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats

Al-Kuraishy

Al-Hussaniy²,

Al-Gareeb

et al. 2022

Front. Pharmacol.

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Doxorubicin (DOX) is an anticancer agent for treating solid and soft tissue malignancies. However, the clinical use of DOX is restricted by cumulative, dose-dependent cardiotoxicity. Therefore, the present study aimed to assess the cardioprotective effects of P. ginseng C. A. Mey, febuxostat, and their combination against DOX-induced cardiotoxicity. Thirty-five Sprague Dawley male rats were used in this study. The animals were randomly divided into five groups, with seven rats per group. The control group received normal saline, the induced group received DOX only, and the treated group received P. ginseng, febuxostat, and their combination before DOX treatment. Biomarkers of acute cardiac toxicity were assessed in each group. Results showed that treatment with the combination of febuxostat and P. ginseng before DOX led to a significant improvement in the biomarkers of acute DOX-induced cardiotoxicity. In conclusion, the combination of P. ginseng and febuxostat produced more significant cardioprotective effects against DOX-induced cardiotoxicity when compared to either P. ginseng or febuxostat when used alone. The potential mechanism of this combination was mainly mediated by the anti-inflammatory and antioxidant effects of P. ginseng and febuxostat.

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Section: Discussionmentioning

confidence: 64%

Combination of Panax ginseng C. A. Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats

Al-Kuraishy

Al-Hussaniy²,

Al-Gareeb

et al. 2022

Front. Pharmacol.

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“…However, so far carbon-based nonozymatic activities are limited to oxidase, peroxidase, superoxide dismutase and catalase activities [50,51]. The antitumor effect developed by nanozymes with oxidizing characteristics can be attributed to the reactive oxygen species (ROS) [50]. Different elements, natural products or metal-doped carbon-based nanozymes are developed to be used as sensor, anti-cancer therapeutic reagents [52][53][54][55][56].…”

Section: Discussionmentioning

confidence: 99%

“…Carbon-based nanozyme materials have been well studied and applied to various practices regarding their antitumor and antimicrobial effects. However, so far carbon-based nonozymatic activities are limited to oxidase, peroxidase, superoxide dismutase and catalase activities [50,51]. The antitumor effect developed by nanozymes with oxidizing characteristics can be attributed to the reactive oxygen species (ROS) [50].…”

Section: Discussionmentioning

confidence: 99%

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

Fan

Sun

Dukenbayev

et al. 2022

Preprint

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Background Quantum nanodots especially carbon nanoparticles (CNPs) have been widely studied in biomedicine in imaging, drug delivery, but anti-cancer mechanisms remain elusive. Methods Here we studied on a type of cell death induced by food (beet, soybean) derived CNPs in cancer cells and tested whether CNPs induced DNA damage and resistant to anticancer agent PARP inhibitor (PARPi) could be overcome by quantum calculation, SEM, TEM, AFM, FT-IR, soft agar assay, and cytotoxicity assay. Results At high doses, CNPs derived from beet lead to a pop-like apoptosis of cells. Quantum mechanical calculation confirmed CNPs binding with phosphate groups as well as DNA bases. At low doses, CNPs develop PARPi drug resistance through interactions between CNPs and PARPi. A synergistic drug effect was achieved with the combination of phosphatase inhibitor (PPi), PARPi and CNPs. This is corroborated by the fact that a sulfur modulated CNPs which exhibit super high phosphatase nanozyme activity abrogated the CNPs induced colony formation in anchorage-independent cancer cell growth. Conclusion Thus, our data suggest the CNPs intrinsic nanozyme activity of phosphatase may crosstalk with drug resistance, which can be reversed upon modulations.

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“…The size distribution, zeta potential, and PDI of the micelles were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZS-90 (Malvern, United Kingdom). Data were recorded at 25°C with a scattering angle of 90° ( Nazarbek et al, 2021 ; Xie et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Preparation and Evaluation of the Cytoprotective Activity of Micelles with DSPE-PEG-C60 as a Carrier Against Doxorubicin-Induced Cytotoxicity

Ding

et al. 2022

Front. Pharmacol.

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To deliver doxorubicin (DOX) with enhanced efficacy and safety in vivo, fullerenol-modified micelles were prepared with the amphiphilic polymer DSPE-PEG-C60 as a carrier, which was synthesized by linking C60(OH)22 with DSPE-PEG-NH2. Studies of its particle size, PDI, zeta potential, and encapsulation efficiency were performed. DOX was successfully loaded into the micelles, exhibiting a suitable particle size [97 nm, 211 nm, 260 nm, vector: DOX = 5:1, 10:1; 15:1 (W/W)], a negative zeta potential of around −30 mv, and an acceptable encapsulation efficiency [86.1, 95.4, 97.5%, vector: DOX = 5:1, 10:1; 15:1 (W/W)]. The release behaviors of DOX from DSPE-PEG-C60 micelles were consistent with the DSPE-PEG micelles, and it showed sustained release. There was lower cytotoxicity of DSPE-PEG-C60 micelles on normal cell lines (L02, H9c2, GES-1) than free DOX and DSPE-PEG micelles. We explored the protective role of DSPE-PEG-C60 on doxorubicin-induced cardiomyocyte damage in H9c2 cells, which were evaluated with a reactive oxygen species (ROS) assay kit, JC-1, and an FITC annexin V apoptosis detection kit for cellular oxidative stress, mitochondrial membrane potential, and apoptosis. The results showed that H9c2 cells exposed to DSPE-PEG-C60 micelles displayed decreased intracellular ROS, an increased ratio of red fluorescence (JC-1 aggregates) to green fluorescence (JC-1 monomers), and a lower apoptotic ratio than the control and DSPE-PEG micelle cells. In conclusion, the prepared DOX-loaded DSPE-PEG-C60 micelles have great promise for safe, effective tumor therapy.

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Nano-evolution and protein-based enzymatic evolution predicts novel types of natural product nanozymes of traditional Chinese medicine: cases of herbzymes of Taishan-Huangjing (Rhizoma polygonati) and Goji (Lycium chinense)

Abstract: Nanozyme and natural product-derived herbzyme have been identified in different enzyme types simulating the natural protein-based enzyme function. How to explore and predict enzyme types of novel nanozyme when synthesized...

Cited by 16 publications

References 29 publications

Combination of Panax ginseng C. A. Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats

Combination of Panax ginseng C. A. Mey and Febuxostat Boasted Cardioprotective Effects Against Doxorubicin-Induced Acute Cardiotoxicity in Rats

Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells

Preparation and Evaluation of the Cytoprotective Activity of Micelles with DSPE-PEG-C60 as a Carrier Against Doxorubicin-Induced Cytotoxicity

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