Nano-emulsion of mangosteen rind extract in a mucoadhesive patch for periodontitis regenerative treatment: An in vivo study

Aljuanid, Mohammed Ahmed; Qaid, Huda Rashad; Lashari, Dur Muhammad; Ridwan, Rini Devijanti; Budi, Hendrik Setia; Alkadasi, Baleegh Abdulraoof; Ramadhani, Yeka; Rahmasari, Riski Rahayu Putri

doi:10.1016/j.jtumed.2022.03.003

Cited by 5 publications

(7 citation statements)

References 47 publications

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“…12 26 29 The IL-10 has an important role in periodontitis, 30 which is able to control the number of bacteria, 31 regulation of pro-inflammatory cytokine, and increase OPG and osteoclastogenesis. 32 The previous study by Aljunaid et al and Lashari et al also showed that the EGCG is able to stimulate the IL-10 expression 11 12 and exhibit the RANKL as an osteoclast marker. 10 As previously mentioned, IL-10 suppresses several key proinflammatory cytokines that are clinically validated to participate in the pathogenesis of any disease of the oral mucosa, especially periodontitis.…”

Section: Discussionmentioning

confidence: 92%

“…The finding showed that EGCG inhibits the process of alveolar bone damage through RANKL, 10 TNF-α, 11 and increases osteoprotegerin (OPG), 10 RANK, 10,11 and IL-10. 11,12 The effect is similar to α-mangosteen, which is able to stimulate some growth factors like tumor growth factor-β, 13 and TRAP5b 14 and also inhibit the growth of etiological bacteria 15,16 and RUNX2. 14 Periodontitis is a disease of the periodontal tissues that is characterized by damage to the ligaments and surrounding alveolar bone.…”

Section: Introductionmentioning

confidence: 99%

“…The previous study of the EGCG and α-mangosteen in periodontitis has developed. The finding showed that EGCG inhibits the process of alveolar bone damage through RANKL, 10 TNF-α, 11 and increases osteoprotegerin (OPG), 10 RANK, 10 11 and IL-10. 11 12 The effect is similar to α-mangosteen, which is able to stimulate some growth factors like tumor growth factor-β, 13 and TRAP5b 14 and also inhibit the growth of etiological bacteria 15 16 and RUNX2.…”

Section: Introductionmentioning

confidence: 99%

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The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

et al. 2023

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Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecules from SHED, namely cytokines, chemokines, and growth factors. The metabolite can be used in various regenerative therapy based on cell-free immunomodulatory potential effects, like interleukin 10 (IL-10) and LL37. This molecule can stimulate with epigallocatechin gallate (EGCG) and α-mangosteen and has been proven to have anti-inflammatory and antibacterial effects. This study aimed to identify the effect of EGCG and α-mangosteen to SHED metabolite, called SHED-IL10 and SHED-LL37, from six passages to obtain the optimum stimulation and able to use as periodontitis regeneration treatment. Materials and Methods The six different passages of SHED were prepared in Dulbecco's modified Eagle medium and added with EGCG 80% (10 μM), EGCG 95% (10 μM), or α-mangosteen (10 μM). After a 24 hours incubation, each passage was measured with the metabolite concentration, SHED-IL10 and SHED-LL37, with human IL-10 and LL37 using enzyme-linked immunosorbent assay. Each different concentration was then analyzed statistically. Results The addition of EGCG 95% is able to stimulate the SHED-IL10 optimum concentration in passage 1 (p < 0.01). But, in the different conditions, the addition of EGCG 80%, EGCG 95%, and α-mangosteen was able to stimulate the SHED-LL37 optimum concentration in passage 2 (p < 0.001). Conclusion The addition of EGCG and α-mangosteen can stimulate the SHED-IL10 and SHED-LL37 concentrations. These two metabolites are promising as regenerative therapy through anti-inflammatory and antibacterial properties.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

et al. 2023

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“…47 These results are in line with other reported research showing that xanthones can damage the mechanical stability of the S. mutans biofilm and inhibit glucosyltransferase enzyme activity, which results in the inhibition of biofilm growth. 47 Mangosteen pericarp extract has demonstrated promise as a plaque inhibitor in mouthwashes, 48,49 and a soluble film of xanthone α-mangostin exhibited antimicrobial activity against oral pathogens and inhibited biofilm formation in vitro. 13,42 Once the effectiveness of MGTN as an antiseptic either in solution or in formulation was confirmed, we also evaluated its safety through biocompatibility assessment, confirming the lack of cytotoxicity on the 2D culture in vitro model of human gingival fibroblasts by the low LDH activity levels and improved metabolic activity induced by MGTN treatment.…”

Section: Discussionmentioning

confidence: 99%

“…mutans biofilm and inhibit glucosyltransferase enzyme activity, which results in the inhibition of biofilm growth . Mangosteen pericarp extract has demonstrated promise as a plaque inhibitor in mouthwashes, , and a soluble film of xanthone α-mangostin exhibited antimicrobial activity against oral pathogens and inhibited biofilm formation in vitro . , …”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Mangostanin as an Antimicrobial and Biocompatible Topical Antiseptic for Skin and Oral Tissues

Munar-Bestard,

Rodríguez-Fernández,

Ramis

et al. 2024

ACS Pharmacol. Transl. Sci.

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Skin and oral tissue infections pose significant health challenges worldwide, necessitating the exploration of new antiseptic agents that are both effective and biocompatible. This study evaluated the antibacterial efficacy and biocompatibility of mangostanin (MGTN), a xanthone derived from Garcinia mangostana L., against commercial antiseptics across various bacterial strains (Porphyromonas gingivalis, Streptococcus mutans, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and Cutibacterium acnes) and in vitro models of skin and oral tissues. MGTN demonstrated significant antimicrobial activity against all tested pathogens concurrently exhibiting negligible cytotoxic effects on human gingival fibroblasts as well as on threedimensional (3D) models of human epidermis and oral epithelium. Furthermore, using pooled human saliva, MGTN effectively inhibited plaque biofilm formation, suggesting its potential as a natural, biocompatible antiseptic for skin and oral health applications. These findings position MGTN as a promising candidate for further development into antiseptic formulations, offering a natural alternative to current synthetic options.

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Retrospective analysis of cefpodoxime proxetil dispersible tablets in the treatment of respiratory tract infection in children and study of the antibacterial effect of cefpodoxime proxetil nanoemulsion

Xue

Tian

2023

mat express

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A study was conducted to determine the efficacy and safety of cefpodoxime proxetil (CFP) dispersible tablets in the treatment of respiratory tract infection in children and to analyze the antibacterial effect of cefpodoxime proxetil nanoemulsion (CFP-NE). A retrospective analysis was conducted on 118 children with upper respiratory tract infection who received antibiotic treatment between March 2018 and March 2021. The control group was treated with cefradine and the CFP group was treated with CFP dispersible tablets. The serum indexes and bacteriological test results of the two groups were collected before and after treatment, and the bacterial clearance rate, infection control, and occurrence of adverse reactions were observed and compared between the two groups. Cefpodoxime proxetil nanoemulsion (CFP-NE) was prepared and its surface characteristics were observed. CFP-NE and CFP were used to treat infections by multiple strains of bacteria and the antibacterial effect and minimum inhibitory concentration (MIC) of CFP-NE were observed. The bacterial strain clearance rate of the CFP group was 96.2% and the total infection control rate was 94.92%. The bacterial strain clearance rate of the control group was 88.9% and the total infection control rate was 81.36%. Serum index detection showed that antibiotic treatment could reduce serum indexes of amyloid A, C-reactive protein, procalcitonin, and other indexes and CFP had better efficacy. There were only four cases of adverse reactions after treatment with CFP, a result similar to that of other antibiotics. The prepared CFP-NE system was stable, the particles were uniform, and the diameter of the inhibitory zone against pathogenic Escherichia coli and Salmonella was larger than that of the CFP group. The MIC of CFP-NE was significantly lower than the MIC of the CFP group (P < 0.05). CFP dispersible tablets are safe and effective for the treatment of respiratory tract infection in children and the antibacterial effect of CFP-NE is greater than that of CFP.

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Nano-emulsion of mangosteen rind extract in a mucoadhesive patch for periodontitis regenerative treatment: An in vivo study

Cited by 5 publications

References 47 publications

The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

In Vitro Evaluation of Mangostanin as an Antimicrobial and Biocompatible Topical Antiseptic for Skin and Oral Tissues

Retrospective analysis of cefpodoxime proxetil dispersible tablets in the treatment of respiratory tract infection in children and study of the antibacterial effect of cefpodoxime proxetil nanoemulsion

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