Nano Delivery of Antiviral Plant Bioactives as Cancer Therapeutics

Shanmugam, Haripriya; Priya, Badma; Swetha, Manickam Senguttuvan; Semalaiyappan, Janani

doi:10.1201/9781003136644-18

Cited by 2 publications

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“…As a multi-disciplinary approach, the present study focused on designating phytochemicals from leaf extracts of soursop (Annona muricata), a plant known for its broad spectrum anti-cancer activity against osteosarcoma or bone cancer through analytical and in silico chemistry approach with con rmation from in vitro cell line studies. Annona muricata L, widely known as graviola or soursop is considered to have potential phytochemicals like phenols, saponins, terpenoids, avonoids, isoquinoline alkaloids and annonaceous acetogenins (Alali et al 1999;Bonneau et al 2017;Shanmugam et al 2022) that are reported to be effective against various cancer cell lines (Pieme et al 2014). Several research works have con rmed that the entire soursop tree exhibits selective and signi cant anticancer activity, with leaves being the most potent (Mishra et al 2013;Rupprecht et al 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, leaf extracts exhibited void/negative impact on normal cells, while showing selective toxicity towards cancerous cells (de Sousa and Vieira, 2010; Oberlies et al 1995). Of late, nano delivery of plant phytochemicals with antiviral properties are explored in cancer treatment to increase the therapeutic e ciency and to overcome the limitations of chemotherapy drugs (Shanmugam et al 2022).…”

Section: Introductionmentioning

confidence: 99%

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Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

Shanmugam,

Narmadha,

Ravikumar

et al. 2024

Chem. Pap.

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Soursop (Annona muricata) is being used in treating various types of cancers and there is no report on effect of soursop leaf phytochemicals against osteosarcoma. Current study identi ed 28 metabolites from ethyl acetate leaf (EAL) extract through GC-MS chemopro ling and subjected to in silico analysis against the potential protein target, Platelet Derived Growth Factor Receptor α (PDGFRA) of osteosarcoma, including Absorption, Distribution, Metabolism, and Excretion and Toxicity (ADMET) analysis to identify possible hit compounds. This resulted in three hit leaf bioactives namely, 2'-hydroxy-5'-methyl chalcone, linoleic acid and annonacin showing good binding a nity with a docking score of -7.4, -7.0 and -6.9 kcal/mol respectively. With ADMET analysis, 2'-hydroxy-5'-methyl chalcone and linoleic acid obeyed Lipkinsi's rule of ve, whereas annonacin showed slight violation. Among the three docked complexes, annonacin exhibited good stability during molecular dynamic simulation performed with PDGFRA. Hence, concentration of the key marker compound, annonacin in EAL concentrate is found to be 5.032± 0.13 mg/g of leaf sample. Further, EAL concentrate exhibited cytotoxicity (IC 50 value) on MG-63 osteosarcoma cells in vitro for concentrations ranging from 10 to 25 µg/mL and nuclear imaging of osteoblast cells treated with EAL concentrate at 25 µg/mL concentration exhibited typical symptoms of apoptosis. In vitro cytotoxicity along with nuclear imaging con rmed EAL concentrate from soursop to be a potential drug candidate in developing new anti-cancer agent against osteosarcoma.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

Shanmugam,

Narmadha,

Ravikumar

et al. 2024

Chem. Pap.

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Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

Shanmugam,

Narmadha,

Ravikumar

et al. 2023

Preprint

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Soursop (Annona muricata) is being used in treating various types of cancers and there is no report on effect of soursop leaf phytochemicals against osteosarcoma. Current study identified 28 metabolites from ethyl acetate leaf (EAL) extract through GC-MS chemoprofiling and subjected to in silico analysis against the potential protein target, Platelet Derived Growth Factor Receptor α (PDGFRA) of osteosarcoma, including Absorption, Distribution, Metabolism, and Excretion and Toxicity (ADMET) analysis to identify possible hit compounds. This resulted in three hit leaf bioactives namely, 2’- hydroxy-5’-methyl chalcone, linoleic acid and annonacin showing good binding affinity with a docking score of -7.4, -7.0 and – 6.9 kcal/mol respectively. With ADMET analysis, 2’- hydroxy-5’-methyl chalcone and linoleic acid obeyed Lipkinsi’s rule of five, whereas annonacin showed slight violation. Among the three docked complexes, annonacin exhibited good stability during molecular dynamic simulation performed with PDGFRA. Hence, concentration of the key marker compound, annonacin in EAL concentrate is found to be 5.032± 0.13 mg/g of leaf sample. Further, EAL concentrate exhibited cytotoxicity (IC50 value) on MG-63 osteosarcoma cells in vitro for concentrations ranging from 10 to 25 µg/mL and nuclear imaging of osteoblast cells treated with EAL concentrate at 25 µg/mL concentration exhibited typical symptoms of apoptosis. In vitro cytotoxicity along with nuclear imaging confirmed EAL concentrate from soursop to be a potential drug candidate in developing new anti-cancer agent against osteosarcoma.

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Nano Delivery of Antiviral Plant Bioactives as Cancer Therapeutics

Cited by 2 publications

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Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

Anti-proliferative effect of leaf phytochemicals of soursop (Annona muricata L.) against human osteosarcoma in vitro

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