Nano-copper Enhances Gene Regulation of Non-specific Immunity and Antioxidative Status of Fish Reared Under Multiple Stresses

Kumar, Neeraj; Thorat, Supriya Tukaram; Gite, Archana; Patole, Pooja Bapurao

doi:10.1007/s12011-023-03575-6

Cited by 8 publications

(10 citation statements)

References 85 publications

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“…Copper sulfate and Cu‐NPs were used for this bioassay, with a concentration of 100 mg L −1 for copper sulfate (HiMedia). Cu‐NPs were biologically synthesized using fish gill tissue 11,12 . To determine the LC 50 , a range‐finding test was performed for both Cu and Cu‐NPs using concentrations of 5, 10, 15, 20, and 25 mg L −1 for Cu, and 2, 4, 6, 8, and 10 mg L −1 for Cu‐NPs.…”

Section: Methodsmentioning

confidence: 99%

“…The color of the solution changed from blue to reddish‐brown, indicating the presence of Cu‐NPs. The solution was then centrifuged to collect the Cu‐NPs using ethanol 11,12 …”

Section: Methodsmentioning

confidence: 99%

“…Tissues such as liver, gill, kidney, and brain were dissected, thoroughly cleaned, and homogenized in 0.25 M chilled sucrose solution and 1 mM EDTA using a tissue homogenizer (Omni Tissue Master Homogenizer, Kennesaw, GA). The resulting tissue homogenates were centrifuged at 6000Âg for 20 min at 4 C and stored at À80 C. 11,12…”

Section: Sample Preparation For Enzymatic Analysismentioning

confidence: 99%

“…Excessive use of Cu nanoparticles (Cu-NPs) can intensify contamination in aquatic ecosystems, negatively affecting fish by impeding their growth and development. 11,12 Due to their non-degradability, both Cu and Cu-NPs accumulate in aquatic systems and within fish bodies. 13 This accumulation leads to altered morphology, fatty metamorphosis in liver tissue, hematopoietic tissue destruction, kidney necrosis, gill tissue changes, and elevated cortisol levels in fish.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Cu is utilized in the form of nano‐materials in diverse sectors, further impacting aquatic systems. Excessive use of Cu nanoparticles (Cu‐NPs) can intensify contamination in aquatic ecosystems, negatively affecting fish by impeding their growth and development 11,12 . Due to their non‐degradability, both Cu and Cu‐NPs accumulate in aquatic systems and within fish bodies 13 .…”

Section: Introductionmentioning

confidence: 99%

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Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Kumar,

Gismondi,

Reddy

2023

Environmental Toxicology

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The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu‐NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50) for Cu and Cu‐NPs were determined as 8.04 and 3.85 mg L−1, respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L−1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L−1 for Cu‐NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione‐s‐transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu‐NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu‐NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu‐NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu‐NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu‐NPs, induce toxicity and disrupt cellular metabolic activities in fish.

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Section: Methodsmentioning

confidence: 99%

“…The color of the solution changed from blue to reddish‐brown, indicating the presence of Cu‐NPs. The solution was then centrifuged to collect the Cu‐NPs using ethanol 11,12 …”

Section: Methodsmentioning

confidence: 99%

Section: Sample Preparation For Enzymatic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Kumar,

Gismondi,

Reddy

2023

Environmental Toxicology

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Potential role of dietary zinc on gene regulation of growth performance and immunity in Pangasianodon hypophthalmus against multiple stresses

Kumar,

Thorat,

Chavhan

et al. 2024

Aquaculture

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Manganese nanoparticles control the gene regulations against multiple stresses in Pangasianodon hypophthalmus

Kumar,

Thorat,

Singh

et al. 2023

Sci Rep

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Ammonia and arsenic pollution, along with the impact of climate change, represent critical factors influencing both the quantity and quality of aquaculture production. Recent developments have underscored the significance of these issues, as they not only disrupt aquatic ecosystems but also have far reaching consequences for human health. To addressed above challenges, an experiment was conducted to delineate the potential of manganese nanoparticles (Mn-NPs) to mitigate arsenic and ammonia pollution as well as high temperature stress in Pangasianodon hypophthalmus. The fish were exposed to different combination of arsenic and ammonia pollution as well as high temperature stress, while simultaneously incorporating diets enriched with Mn-NPs. The inclusion of Mn-NPs at 3 mg kg−1 in the diet led to a noteworthy downregulation of cortisol and HSP 70 gene expression, indicating their potential in mitigating stress responses. Furthermore, immune related gene expressions were markedly altered in response to the stressors but demonstrated improvement with the Mn-NPs diet. Interestingly, the expression of inducible nitric oxide synthase (iNOS), caspase (CAS), metallothionine (MT) and cytochrome P450 (CYP450) genes expression were prominently upregulated, signifying a stress response. Whereas, Mn-NPs at 3 mg kg−1 diet was significantly downregulated theses gene expression and reduces the stress. In addition to stress-related genes, we evaluated the growth-related gene expressions such as growth hormone (GH), growth hormone regulator 1 (GHR1 and GHRβ), Insulin like growth factor (IGF1 and IGF2) were significantly upregulated whereas, myostatin and somatostatin were downregulated upon the supplementation of dietary Mn-NPs with or without stressors in fish. The gene expression of DNA damage inducible protein and DNA damage in response to head DNA % and tail DNA % was protected by Mn-NPs diets. Furthermore, Mn-NPs demonstrated a capacity to enhance the detoxification of arsenic in different fish tissues, resulting in reduced bioaccumulation of arsenic in muscle and other tissues. This finding highlights Mn-NPs as a potential solution for addressing bioaccumulation associated risks. Our study aimed to comprehensively examined the role of dietary Mn-NPs in mitigating the multiple stressors using gene regulation mechanisms, with enhancing the productive performance of P. hypophthalmus.

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Nano-copper Enhances Gene Regulation of Non-specific Immunity and Antioxidative Status of Fish Reared Under Multiple Stresses

Cited by 8 publications

References 85 publications

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus

Potential role of dietary zinc on gene regulation of growth performance and immunity in Pangasianodon hypophthalmus against multiple stresses

Manganese nanoparticles control the gene regulations against multiple stresses in Pangasianodon hypophthalmus

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