Nano-composite of poly(-lactide) and surface grafted hydroxyapatite: Mechanical properties and biocompatibility

Hong, Zhongkui; Zhang, Peibiao; He, Chaoliang; Qiu, Xueyu; Liu, Aixue; Chen, Li; Chen, Xuesi; Jing, Xiabin

doi:10.1016/j.biomaterials.2005.04.018

Cited by 411 publications

(252 citation statements)

References 27 publications

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“…To overcome these limitations, HA has been incorporated with natural biomacromolecules such as collagen [6][7][8] and gelatin [9,10], or synthetic polymers such as poly (α-hydroxyl acids) [11][12][13][14][15], poly (ε-caprolactone) (PCL) [16,17], polyamide [18], and polymethylmethacrylate (PMMA) [19] to prepare composites using a variety of methods including surface coating, grafting, direct mixing, and biomimetic precipitation [10,11,[20][21][22][23]. Particularly, polymer/HA nanocomposites have improved mechanical properties and enhanced cell attachment, spreading, and proliferation on their surfaces by adding nano-sized HA to modify the polymer's characteristics and/or strengthen the polymer matrix [24,25]. Oftentimes, these nanocomposites still cannot achieve the targeted compressive strength and modulus for loadbearing applications because the matrix polymers themselves do not have sufficient mechanical properties to be improved upon.…”

Section: Introductionmentioning

confidence: 99%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/ HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

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Section: Introductionmentioning

confidence: 99%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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“…with PLLA was obtained, and improved the mechanical property of the blend with PLLA and PLGA to a greater extent (Hong et al, 2005;Zhang et al, 2009). The composites of g-HA/PLLA (or PLGA) show a wide variety of applications in bone fixing.…”

Section: Discussionmentioning

confidence: 94%

“…HA/PLLA composites have shown some advantages in mechanical property, biocompatibility, and osteoconductivity (Hong et al, 2005). In our previous work, a novel nanocomposite of HA grafted …”

Section: Discussionmentioning

confidence: 99%

“…HA nanoparticles (100 nm in length and 20-40 nm in width, atomic ratio calcium (Ca)/phosphorous (P)≈1.67) were surface-grafted with PLLA according to the method of our previous study (Hong et al, 2005), and the amount of grafted polymer was approximately 5%-6% (w/w) determined by thermal gravimetric analysis (TGA; TA Instruments TGA500, USA). The nanocomposite of g-HA/PLGA was prepared by 10% (w/w) g-HA, and suspended in 10% (w/v) PLGA/chloroform solution.…”

Section: Preparation Of Foam Scaffoldsmentioning

confidence: 99%

“…In our previous work, we reported a novel composite of grafted HA (g-HA)/poly(L-lactide) (PLLA) (or PLGA) (Hong et al, 2005;. The g-HA was obtained through the hydroxyl groups on the surface of the HA nanoparticles grafted with PLLA, and its blends with PLLA or PLGA were achieved with a higher level of mechanical properties and stability.…”

Section: Introductionmentioning

confidence: 99%

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Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration

Zhang

Wang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: A new therapeutic strategy using nanocomposite scaffolds of grafted hydroxyapatite (g-HA)/ poly(lactide-co-glycolide) (PLGA) carried with autologous mesenchymal stem cells (MSCs) and bone morphogenetic protein-2 (BMP-2) was assessed for the therapy of critical bone defects. At the same time, tissue response and in vivo mineralization of tissue-engineered implants were investigated. Methods: A composite scaffold of PLGA and g-HA was fabricated by the solvent casting and particulate-leaching method. The tissue-engineered implants were prepared by seeding the scaffolds with autologous bone marrow MSCs in vitro. Then, mineralization and osteogenesis were observed by intramuscular implantation, as well as the repair of the critical radius defects in rabbits. Results: After eight weeks post-surgery, scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) revealed that g-HA/PLGA had a better interface of tissue response and higher mineralization than PLGA. Apatite particles were formed and varied both in macropores and micropores of g-HA/PLGA. Computer radiographs and histological analysis revealed that there were more and more quickly formed new bone formations and better fusion in the bone defect areas of g-HA/PLGA at 2-8 weeks post-surgery. Typical bone synostosis between the implant and bone tissue was found in g-HA/PLGA, while only fibrous tissues formed in PLGA. Conclusions: The incorporation of g-HA mainly improved mineralization and bone formation compared with PLGA. The application of MSCs can enhance bone formation and mineralization in PLGA scaffolds compared with cell-free scaffolds. Furthermore, it can accelerate the absorption of scaffolds compared with composite scaffolds.

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Nano-composite of poly(-lactide) and surface grafted hydroxyapatite: Mechanical properties and biocompatibility

Cited by 411 publications

References 27 publications

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Tissue-engineered composite scaffold of poly(lactide-co-glycolide) and hydroxyapatite nanoparticles seeded with autologous mesenchymal stem cells for bone regeneration

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