Nano co-immobilization of α-amylase and maltogenic amylase by nanomagnetic combi-cross-linked enzyme aggregates method for maltose production from corn starch

Torabizadeh, Homa; Montazeri, Ensieh

doi:10.1016/j.carres.2019.107904

Cited by 32 publications

(6 citation statements)

References 34 publications

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“…The appropriate mass ratio of enzymes in the co-immobilized system would reduce the precipitation of intermediate products and accumulate the nal product. 50,51 Thus, in this study, 1 : 10 (UGT1 to UGT2) was selected as an optimum mass ratio of the two enzymes to further study the effects of the other process variables. The co-immobilized UGTs showed the highest catalytic activity at 37 C (Fig.…”

Section: Effect Of Temperature and Ph On Activity And Reusability Of The Immobilized Ugtsmentioning

confidence: 99%

Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade

Wang

Liu

et al. 2021

RSC Adv.

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Section: Effect Of Temperature and Ph On Activity And Reusability Of The Immobilized Ugtsmentioning

confidence: 99%

Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade

Wang

Liu

et al. 2021

RSC Adv.

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“…Strategies for controlling the localization of different covalently co-immobilized enzymes have been reported. For instance, proximity between two different enzymes in dual-enzyme catalysts has been achieved by obtaining protein conjugates [74] or combi-CLEAs [68] and further immobilizing them in into solid supports covalently. A sophisticated strategy was reported by Tan and co-workers [76] to co-immobilize monooxygenase of Pseudomonas putida (P450cam) together with P. putida ferredoxin (PdX) and ferredoxin reductase (PdR).…”

Section: Thiol-disulphide Interchangementioning

confidence: 99%

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

et al. 2021

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During recent decades, the use of enzymes or chemoenzymatic cascades for organic chemistry has gained much importance in fundamental and industrial research. Moreover, several enzymatic and chemoenzymatic reactions have also served in green and sustainable manufacturing processes especially in fine chemicals, pharmaceutical, and flavor/fragrance industries. Unfortunately, only a few processes have been applied at industrial scale because of the low stabilities of enzymes along with the problematic processes of their recovery and reuse. Immobilization and co-immobilization offer an ideal solution to these problems. This review gives an overview of all the pathways for enzyme immobilization and their use in integrated enzymatic and chemoenzymatic processes in cascade or in a one-pot concomitant execution. We place emphasis on the factors that must be considered to understand the process of immobilization. A better understanding of this fundamental process is an essential tool not only in the choice of the best route of immobilization but also in the understanding of their catalytic activity.

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“…Enzyme immobilization is a widely accepted approach to overcoming these hurdles . Although immobilization aims to improve the stability and reusability of the biocatalyst, its extent explicitly depends on the intrinsic characteristics of the support matrix employed. ,, …”

Section: Introductionmentioning

confidence: 99%

“…9 Although immobilization aims to improve the stability and reusability of the biocatalyst, its extent explicitly depends on the intrinsic characteristics of the support matrix employed. 4,10,11 Nanotechnological interventions in biotechnology have forecasted the potential of "nanobiocatalysis" as a sustainable tool in food waste valorization. 12−14 The "waste-to-wealth" concept via an enzyme−nanomaterial system has been synchronized to produce industrially relevant biochemical products, viz.…”

Section: ■ Introductionmentioning

confidence: 99%

Magnetic Halloysite Nanotube/α-Amylase Based Nanobiocatalytic Transformation of Food Processing Waste into an Active Fermentation Ingredient

Sillu

Agnihotri

Reddy

2022

ACS Sustainable Chem. Eng.

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The concept of “nanobiocatalysis” creates exciting opportunities for improving enzyme performance via immobilization onto nanomaterials. A nanobiocatalyst consisting of magnetic halloysite nanotube (MHNT)/α-amylase was evaluated to transform food processing waste into an active fermentation medium formulation for low-cost bioprocessing. A high loading of α-amylase (185.5 mg (g of support)−1) was achieved on the surface of MHNTs through polydopamine functionalization. We validated the establishment of an enzyme-support system retaining >89% catalytic activity (27332 IU (g of support)−1) with improved enzyme handling (>99.1% recovery) and reusability (>56% activity, 10 cycles). MHNTs remarkably improved the enzyme kinetics and thermodynamic characteristics along with operational and storage stabilities and mitigated the likely inhibitory effects of cellulose/metal ions as contaminants. In addition to facilitating a continuous production of reducing sugars from the extracted starch over 72 h, the nanobiocatalyst was equally effective in preparing a nutritive food waste hydrolysate as a fermentable medium substitute for batch culturing of E. coli and a single-cell protein (A. niger). The commercial relevance of waste hydrolysate was also investigated to promote calcite precipitation via Bacillus sp. induced biocementation. We evidenced that nanobiocatalyst-assisted “starch depolymerization” released more nutritional components into the hydrolysate suspension, easily accessible to growing microbial cultures.

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Nano co-immobilization of α-amylase and maltogenic amylase by nanomagnetic combi-cross-linked enzyme aggregates method for maltose production from corn starch

Cited by 32 publications

References 34 publications

Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade

Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade

Multicatalytic Hybrid Materials for Biocatalytic and Chemoenzymatic Cascades—Strategies for Multicatalyst (Enzyme) Co-Immobilization

Magnetic Halloysite Nanotube/α-Amylase Based Nanobiocatalytic Transformation of Food Processing Waste into an Active Fermentation Ingredient

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