Nano- and Microscale Delivery Systems for Cardiovascular Therapy

Waters, Renae; Maloney, Ryan; Ranganath, Sudhir H.; Hsieh, Hsin-Yi; Paul, Arghya

doi:10.1007/978-3-319-20726-1_13

Cited by 1 publication

(2 citation statements)

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“…Most of the CNT derivatives are impregnated in polymeric constructs through physical encapsulation or entrapment which consequences unstable physiochemical properties of fabricated composites 4,5,11 . To solve this, it is expected to incorporate CNT stably in functional construct through covalent bonds for promotion of cardiomyocyte growth and functions in a desirable way 12,13 . Hence, we aim to covalently immobilize CNT in biomimetic hydrogel membrane in the form of cell‐laden microcapsule through the coaxial microfluidic system and enzyme‐mediated crosslinking 2,14,15 …”

Section: Introductionmentioning

confidence: 99%

“…4,5,11 To solve this, it is expected to incorporate CNT stably in functional construct through covalent bonds for promotion of cardiomyocyte growth and functions in a desirable way. 12,13 Hence, we aim to covalently immobilize CNT in biomimetic hydrogel membrane in the form of cell-laden microcapsule through the coaxial microfluidic system and enzyme-mediated crosslinking. 2,14,15 We recently demonstrated the effectiveness of polysaccharidebased hydrogels such as hyaluronic acid, alginate, pectin, and cellulose as substrates for duplex cell-laden microcapsule production via peroxidase-mediated reaction.…”

Section: Introductionmentioning

confidence: 99%

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Hyaluronic acid/gelatin microcapsule functionalized with carbon nanotube through laccase‐catalyzed crosslinking for fabrication of cardiac microtissue

Sharifisistani

Khanmohammadi

Badali

et al. 2022

J Biomedical Materials Res

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Carbon nanotube (CNT) and gelatin (Gela) molecules are effective substrates in promoting engineered cardiac tissue functions. This study developed a microfluidic‐based encapsulation process for biomimetic hydrogel microcapsule fabrication. The hydrogel microcapsule was produced through a coaxial double orifice microfluidic technique and a water‐in‐oil emulsion system in two sequential processes. The phenol (Ph) substituted Gela (Gela‐Ph) and CNT (CNT‐Ph), respectively as cell‐adhesive and electrically conductive substrates were incorporated in hyaluronic acid (HA)‐based hydrogel through laccase‐mediated crosslinking. The Cardiomyocyte‐enclosing microcapsule fabricated and cellular survival, function, and possible difference in the biological activity of encapsulated cells within micro vehicles were investigated. The coaxial microfluidic method and Lac‐mediated crosslinking reaction resulted in spherical vehicle production in 183 μm diameter at 500 capsules/min speed. The encapsulation process did not affect cellular viability and harvested cells from microcapsule proliferated well likewise subcultured cells in tissue culture plate. The biophysical properties of the designed hydrogel, including mechanical strength, swelling, biodegradability and electroconductivity upregulated significantly for hydrogels decorated covalently with Gela‐Ph and CNT‐Ph. The tendency of the microcapsule for the spheroid formation of cardiomyocytes inside the proposed microcapsule occurred 3 days after encapsulation. Interestingly, immobilized Gela‐Ph and CNT‐Ph promote cellular growth and specific cardiac markers. Overall, the microfluidic‐based encapsulation technology and synthesized biomimetic substrates with electroconductive properties demonstrate desirable cellular adhesion, proliferation, and cardiac functions for engineering cardiac tissue.

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Section: Introductionmentioning

confidence: 99%