Nano- and microparticles for the delivery of polypeptides and proteins

Couvreur, P.; Puisieux, F.

doi:10.1016/0169-409x(93)90046-7

Cited by 255 publications

(119 citation statements)

References 59 publications

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“…32 In general, it is assumed that particles up to about 100-200 nm can be internalized by receptor-mediated endocytosis, while larger particles have to be taken up by phagocytosis. 33 The smaller-sized particles seem to have more efficient interfacial interactions with the cell membrane compared to larger-sized particles. 27 Therapeutic use of lipoparticles is of importance, since the small size of lipoparticles could improve efficacy of particle-based oral drug delivery systems and the smaller size could also improve the efficiency of cellular uptake, making these particles highly feasible for use in oral drug delivery therapy.…”

Section: Discussionmentioning

confidence: 99%

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

Chang¹,

Tai²,

Chiang³

et al. 2011

IJN

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Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)-loaded polyethylene glycol (PEG)-modified liposomes and lipoparticles with high protein entrapment were developed. The lipid formula of the liposomes contained PEGylated lipids and unsaturated fatty acids for enhancing membrane fluidity and effective delivery into cells. The preparation techniques, lipid content, and PEG-modified lipoparticle ratios were evaluated. The PEG-modified lipoparticles prepared by ethanol injection extrusion (100 nm pore size) achieve a population of blank liposomes with a mean size of 125 ± 2.3 nm and a zeta potential of −12.4 ± 1.5 mV. The average particle size of the PEG-modified lipoparticles was 133.7 ± 8.6 nm with a zeta potential of +13.3 mV. Lipoparticle conformation was determined using transmission electron microscopy and field-emission scanning electron microscopy. The FITC-BSA encapsulation efficiency was dramatically increased from 19.0% for liposomes to 59.7% for lipoparticles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results confirmed the preparation process, and an 8-hour leaching test did not harm the protein structure. Once prepared, the physical and chemical stability of the PEG-modified lipoparticle formulations was satisfactory over 90 days. In vitro retention tests indicated that the 50% retention time for the protein-containing lipoparticles was 7.9 hours, substantially longer than the liposomes at 3.3 hours. A Caco-2 cell model was used for evaluating the cytotoxicity and cell uptake efficiency of the PEG-modified lipoparticles. At a lipid content below 0.25 mM, neither the liposomes nor the lipoparticles caused significant cellular cytotoxicity ( P < 0.01) and FITC-BSA was significantly taken up into cells within 60 minutes ( P < 0.01).

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Section: Discussionmentioning

confidence: 99%

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

Chang¹,

Tai²,

Chiang³

et al. 2011

IJN

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“…Microparticles are a generic term to mention microcapsules and microspheres which can be made of polymers or lipids (liposomes) with sizes ranging from 1 to 250 µm (ideally <125 µm and exceptionally 1000 µm) [12,13]. This technology is very important in drug delivery.…”

Section: Type Of New Drug Carriers Systemsmentioning

confidence: 99%

Recent Advances in Drug Delivery Systems

Martinho¹,

Damgé²,

Reis³

2011

JBNB

166

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Section: Microparticle-based Systems As Scaffolds and Carriers For Bimentioning

confidence: 99%

“…The physicochemical properties of many peptides and proteins make their entrapment difficult, because inactivation is possible during their incorporation (Couvreur and Puisieux, 1993). Stability, solubility and sensitivity to light, heat, moisture and pH, intermolecular interactions following co-precipitation or gelling, and adsorption and interaction with excipients are parameters that should be investigated in order to succeed in producing a stable association of peptides with particle-based systems (Couvreur and Puisieux, 1993). While encapsulation of peptides and small molecules into biodegradable microspheres can be achieved using several techniques and with different polymers, the encapsulation of proteins still poses major difficulties with respect to obtaining 'infusion-like' or continuous-release profiles with minimal initial burst and sufficient protein loading within the microspheres (Kissel et al, 1996;Morlock et al, 1998).…”

Section: Microparticle-based Systems As Scaffolds and Carriers For Bimentioning

confidence: 99%

Materials in particulate form for tissue engineering. 2. Applications in bone

Silva

Coutinho

Ducheyne

et al. 2007

J Tissue Eng Regen Med

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Materials in particulate form have been the subjects of intensive research in view of their use as drug delivery systems. While within this application there are still issues to be addressed, these systems are now being regarded as having a great potential for tissue engineering applications. Bone repair is a very demanding task, due to the specific characteristics of skeletal tissues, and the design of scaffolds for bone tissue engineering presents several difficulties. Materials in particulate form are now seen as a means of achieving higher control over parameters such as porosity, pore size, surface area and the mechanical properties of the scaffold. These materials also have the potential to incorporate biologically active molecules for release and to serve as carriers for cells. It is believed that the combination of these features would create a more efficient approach towards regeneration. This review focuses on the application of materials in particulate form for bone tissue engineering. A brief overview of bone biology and the healing process is also provided in order to place the application in its broader context. An original compilation of molecules with a documented role in bone tissue biology is listed, as they have the potential to be used in bone tissue engineering strategies. To sum up this review, examples of works addressing the above aspects are presented. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE R E V I E W A R T I C L E AbstractMaterials in particulate form have been the subjects of intensive research in view of their use as drug delivery systems. While within this application there are still issues to be addressed, these systems are now being regarded as having a great potential for tissue engineering applications. Bone repair is a very demanding task, due to the specific characteristics of skeletal tissues, and the design of scaffolds for bone tissue engineering presents several difficulties. Materials in particulate form are now seen as a means of achieving higher control over parameters such as porosity, pore size, surface area and the mechanical properties of the scaffold. These materials also have the potential to incorporate biologically active molecules for release and to serve as carriers for cells. It is believed that the combination of these features would create a more efficient approach towards regeneration. This review focuses on the application of materials in particulate form for bone tissue engineering. A brief overview of bone biology and the healing process is also provided in order to place the application in its broader context. An original compilation of molecules with a documented role in bone tissue biology is listed, as they have the potential to be used in bone tissue engineering strategies. To sum up this review, examples of works addressing the above aspects are presented.

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Nano- and microparticles for the delivery of polypeptides and proteins

Cited by 255 publications

References 59 publications

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

Recent Advances in Drug Delivery Systems

Materials in particulate form for tissue engineering. 2. Applications in bone

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