Nano‐ and Microfabrication for Engineering Native‐Like Muscle Tissues

Gao, Lei; Yin, Xiaohong; Luo, Yichen; Yang, Huayong; Zhang, Bin

doi:10.1002/smtd.201900669

Cited by 14 publications

(14 citation statements)

References 108 publications

(203 reference statements)

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“…Bulk hydrogels for scaffold-based SMTE were found to successfully provide muscle cell viability, adhesion, and proliferation . However, due to the lack of specific anisotropy architecture, myoblasts tend to proliferate in entangled and disordered layouts, thus hindering the recreation of a functional skeletal muscle tissue construct. , To confer suitable geometrical confinement, which may ultimately result in the generation of a proper cell alignment, first attempts to engineer hydrogels relied on the use of microgrooved hydrogel substrates and micropillars.…”

Section: Hydrogel-based Methods For Myoblast Alignmentmentioning

confidence: 99%

“…Indeed, SMTE strategies offer the possibility to produce in vitro bioartificial constructs, which can potentially support and accelerate the regeneration process . To successfully engineer a skeletal muscle tissue construct, SMTE aims to combine a suitable biomaterial substrate and a proper scaffold design. − As a biomaterial scaffold, synthetic polymers (e.g., polycaprolactone (PCL), polylactic acid (PLA), poly(lactic- co -glycolic acid) (PLGA)) have been proven to efficiently support skeletal muscle tissue regeneration. , However, the cytotoxicity issues mostly related to residual solvents employed during scaffold manufacturing and the lack of cell-supportive nature have prompted alternative biomaterials with higher biocompatibility and biomimetic properties . Among those, hydrogel-based biomaterials are considered promising candidates for engineering skeletal muscle tissue .…”

Section: Introductionmentioning

confidence: 99%

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Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi

Paradiso

Costantini

et al. 2022

ACS Biomater. Sci. Eng.

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The functional capabilities of skeletal muscle are strongly correlated with its well-arranged microstructure, consisting of parallelly aligned myotubes. In case of extensive muscle loss, the endogenous regenerative capacity is hindered by scar tissue formation, which compromises the native muscle structure, ultimately leading to severe functional impairment. To address such an issue, skeletal muscle tissue engineering (SMTE) attempts to fabricate in vitro bioartificial muscle tissue constructs to assist and accelerate the regeneration process. Due to its dynamic nature, SMTE strategies must employ suitable biomaterials (combined with muscle progenitors) and proper 3D architectures. In light of this, 3D fiber-based strategies are gaining increasing interest for the generation of hydrogel microfibers as advanced skeletal muscle constructs. Indeed, hydrogels possess exceptional biomimetic properties, while the fiber-shaped morphology allows for the creation of geometrical cues to guarantee proper myoblast alignment. In this review, we summarize commonly used hydrogels in SMTE and their main properties, and we discuss the first efforts to engineer hydrogels to guide myoblast anisotropic orientation. Then, we focus on presenting the main hydrogel fiber-based techniques for SMTE, including molding, electrospinning, 3D bioprinting, extrusion, and microfluidic spinning. Furthermore, we describe the effect of external stimulation (i.e., mechanical and electrical) on such constructs and the application of hydrogel fiber-based methods on recapitulating complex skeletal muscle tissue interfaces. Finally, we discuss the future developments in the application of hydrogel microfibers for SMTE.

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Section: Hydrogel-based Methods For Myoblast Alignmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Volpi

Paradiso

Costantini

et al. 2022

ACS Biomater. Sci. Eng.

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“…The heart muscle has the effect of regulating the heart rate; it can transmit electrophysiological signals and has a more complex structure. Smooth muscle maintains vascular diameter and is sensitive to electrical stimulation [ 61 , 88 , 89 ]. However, many diseases and injuries, such as myocardial infarction (MI) and volume muscle loss (VML), can cause muscles, which are soft tissues, to be easily injured, not only to the pain of the patient but also to a lot of financial stress.…”

Section: Applications Of Gbns In Soft Tissue Engineeringmentioning

confidence: 99%

Progress in the Development of Graphene-Based Biomaterials for Tissue Engineering and Regeneration

Chen

Weng

2022

Materials

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Over the last few decades, tissue engineering has become an important technology for repairing and rebuilding damaged tissues and organs. The scaffold plays an important role and has become a hot pot in the field of tissue engineering. It has sufficient mechanical and biochemical properties and simulates the structure and function of natural tissue to promote the growth of cells inward. Therefore, graphene-based nanomaterials (GBNs), such as graphene and graphene oxide (GO), have attracted wide attention in the field of biomedical tissue engineering because of their unique structure, large specific surface area, good photo-thermal effect, pH response and broad-spectrum antibacterial properties. In this review, the structure and properties of typical GBNs are summarized, the progress made in the development of GBNs in soft tissue engineering (including skin, muscle, nerve and blood vessel) are highlighted, the challenges and prospects of the application of GBNs in soft tissue engineering have prospected.

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“…Although skeletal muscle is highly regenerative following injury or disease, endogenous self-regeneration is harshly impaired in the conditions of severe volume traumatic muscle loss (VML). Consequently, a growing demand arose in skeletal muscle TE to fully restore the structure and function of lost muscle [ 86 ].…”

Section: Customized Functionalization By Click Chemistry Of Composmentioning

confidence: 99%

Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

et al. 2020

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The development of smart and intelligent regenerative biomaterials for skeletal muscle tissue engineering is an ongoing challenge, owing to the requirement of achieving biomimetic systems able to communicate biological signals and thus promote optimal tissue regeneration. Electrospinning is a well-known technique to produce fibers that mimic the three dimensional microstructural arrangements, down to nanoscale and the properties of the extracellular matrix fibers. Natural and synthetic polymers are used in the electrospinning process; moreover, a blend of them provides composite materials that have demonstrated the potential advantage of supporting cell function and adhesion. Recently, the decellularized extracellular matrix (dECM), which is the noncellular component of tissue that retains relevant biological cues for cells, has been evaluated as a starting biomaterial to realize composite electrospun constructs. The properties of the electrospun systems can be further improved with innovative procedures of functionalization with biomolecules. Among the various approaches, great attention is devoted to the “click” concept in constructing a bioactive system, due to the modularity, orthogonality, and simplicity features of the “click” reactions. In this paper, we first provide an overview of current approaches that can be used to obtain biofunctional composite electrospun biomaterials. Finally, we propose a design of composite electrospun biomaterials suitable for skeletal muscle tissue regeneration.

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Nano‐ and Microfabrication for Engineering Native‐Like Muscle Tissues

Cited by 14 publications

References 108 publications

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering

Progress in the Development of Graphene-Based Biomaterials for Tissue Engineering and Regeneration

Smart ECM-Based Electrospun Biomaterials for Skeletal Muscle Regeneration

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