Nano- and Microbiodevices for High-Performance Separation of Biomolecules

Baba, Yoshinobu

doi:10.15583/jpchrom.2015.030

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…Electrokinetic effects provide both efficient transport and separation of sample components in microfluidic channels. Translation of the separation modes developed for traditional capillary electrophoresis to a microchip platform enables analysis of various types of sample components [3][4][5]. Moreover, miniaturization of device reduces reagent consumption and analysis time.…”

Section: Introductionmentioning

confidence: 99%

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Yamamoto

2021

Chromatography

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Microchip electrophoresis (ME) has emerged as a promising tool for rapid analysis of various sample types and it is one of the most important modules of miniaturized total analysis systems. Electrokinetic effects provide both efficient transport and separation of sample components in microfluidic channels. Moreover, miniaturization of devices drastically reduces reagent consumption, significantly decreases analysis time, and allows for easier parallel screening and automation. However, most microfluidic systems are insensitive, which is a serious problem. In order to increase the sensitivity of ME, several on-line pretreatment applications such as specific and non-specific preconcentration, extraction, and derivatization have been proposed. Here, we review various specific on-line preconcentration methods utilizing in situ photopolymerized polyacrylamide-based preconcentrator gels. These techniques allowed for highly sensitive specific detection of various sample types by ME.

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Section: Introductionmentioning

confidence: 99%

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Yamamoto

2021

Chromatography

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“…The microfluidic scale has several advantages, such as lower consumption of reagents, separation speed and resolution, device portability, high repeatability, multiple assay etc. For this reason, many applications of microfluidic systems have been proposed so far, not only in chemical analysis but also in medical diagnosis, and the food and agricultural industries [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Development of Microfluidic Components for Micro Total Analysis Systems

Naito

2020

Chromatography

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Microfluidic device is capable of reducing amount of sample consumption, shortening the analysis time, and miniaturizing instruments. Many applications of microfluidic devices have been developed not only in chemical analysis but also in medical diagnosis, and the food and agricultural industries. Electrophoresis or chromatography in a microfluidic device has improved the speed, reproducibility and separation resolution. Some of them have been integrated with complex experimental functionality on a small substrate, of which concept is known as micro total analysis systems. To build up a system, microfluidic components that carry out an experimental functionality in a microfluidic channel and novel technology to support the developments of microfluidic components are indispensable. In this review, three-dimensional (3D) fabrication by thiol-ene quick reaction, sample injection with an inkjet ejector, and molecular detection based on electroosmosis are focused briefly. The 3D fabrication realizes to make various 3D microstructures that conventional fabrication method cannot make without specific equipment. The sample injection by using inkjet technology can apply tiny amount of sample solution into multiple microfluidic channel on some precise spots, which leads to rapid analysis with high accuracy. The electroosmosis-based molecular detection indicates a possibility to develop a new portable device without any peripherals for detection or pretreatment for specimen labeling.

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“…Numerous advantages of nano-and micro-biodevices include the separation technologies, HPLC and capillary electrophoretic separation of DNA, nanopillar devices for the ultra-fast separation of DNA and proteins, nanoball materials for the fast separation of wide range of DNA fragments and the nanowire devices for ultra-fast separation of DNA, RNA, and proteins. The studies about these devices have been carried out by Prof. Yoshinobu Baba and the research group [1][2][3][4][5][6][7][8][9][10][11][12]. The nanopillar, nanowall, nanoslit, and nanopore structures were designed by the top down or semiconductor nano-fabrication technology, while the nanoball, nanowire, nanoparticles and the quantum dot structures are designed by the use of bottom up or self-assembled nano-fabrication technology.…”

mentioning

confidence: 99%

“…(a) Device design of nanopillar and (b) nanobiodevices with nanopore, nanopillar, nanowire and nanowell. Adapted from Ref [1]…”

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confidence: 99%

Introductory Chapter: DNA as Nanowires

Srivastava¹

2019

Bio-Inspired Technology [Working Title]

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The integration of nanotechnology with biology and bioengineering has produced many advances with the manipulation of well-defined structures at the nanoscale with high accuracy. DNA molecules can be used for the assembly of devices, for the interconnect joints, or as the device element itself. Sequencespecific DNA detection has been applied in the diagnosis of pathogenic and genetic diseases. The unique physical properties of dots or wires with the remarkable recognition capabilities of DNA could lead to the miniaturization of biological electronics and optical devices, which includes the biosensors and probes. Numerous advantages of nano-and micro-biodevices include the separation technologies, HPLC and capillary electrophoretic separation of DNA, nanopillar devices for the ultra-fast separation of DNA and proteins, nanoball materials for the fast separation of wide range of DNA fragments and the nanowire devices for ultra-fast separation of DNA, RNA, and proteins. The studies about these devices have been carried out by Prof. Yoshinobu Baba and the research group [1][2][3][4][5][6][7][8][9][10][11][12]. The nanopillar, nanowall, nanoslit, and nanopore structures were designed by the top down or semiconductor nano-fabrication technology, while the nanoball, nanowire, nanoparticles and the quantum dot structures are designed by the use of bottom up or self-assembled nano-fabrication technology. These devices are shown in Figure 1.DNA exhibits many other properties; as high stability, adjustable conductance, vast information storage, self-organising capability and programmability. So it is considered as an ideal material for the applications of nanodevices, nanoelectronics and molecular computing. There are several advantages to use DNA for these device designs. The first step of the DNA-based nanotechnology is to attach DNA molecules to the surfaces. It can be done by three different methods: by electrostatic interaction between DNA and a substrate, covalent binding of a chemical group attached to the DNA end and the binding of protein attached at the DNA end to the corresponding antibody immobilized at the surface. Seeman and co-workers [13] have exploited the properties of DNA's molecular recognition to design complex mesoscopic structures based solely on DNA. They used the branched DNA to form stick figures by properly choosing the sequence of the complementary strands. Further macrocycles, DNA quadrilateral, DNA knots, Holliday junctions, and other periodic crystal structures were also designed. DNA-mediated self-assembly of nanostructures has been extended to metallic nanowires [14][15][16]. In a study, DNA as a template was used to grow conducting silver nanowires [14]. The fabrication of gold and silver wires was used with the DNA as a template or skeleton [15]. Nguyen et al. developed an approach for the attachment of DNA to oxidatively open the ends of multiwall carbon nanotube arrays [17]. The carbon wall nanotubes can be used as electrodes to transmit electrical signals or as sensors to detect the con...

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Nano- and Microbiodevices for High-Performance Separation of Biomolecules

Cited by 3 publications

References 32 publications

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

<i>In Situ </i>Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis

Development of Microfluidic Components for Micro Total Analysis Systems

Introductory Chapter: DNA as Nanowires

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