Nano-Anchors with Single Protein Capacity Produced with STED Lithography

Wiesbauer, Moritz; Wollhofen, Richard; Vasić, Borislav; Schilcher, Kurt; Jacak, Jaroslaw; Klar, Thomas A.

doi:10.1021/nl4033523

Cited by 44 publications

(64 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proposed in the early times (Hell, 2004), writing of nanostructures based on the STED and RESOLFT concept has been experimentally realized using photochromic materials (Andrew et al 2009;Fischer et al 2010;Harke et al 2012;Li et al 2009a;Scott et al 2009;Wiesbauer et al 2013;Wollhofen et al 2013) or RSFPs . Analogously to imaging sub-diffraction features, an intensity distribution of a photoswitching laser exhibiting one or several intensity zeros is used to maintain molecules in a reactive ON-state (i.e.…”

Section: Nanoscale Writingmentioning

confidence: 99%

Lens-based fluorescence nanoscopy

Eggeling

Willig

Sahl

et al. 2015

Quart. Rev. Biophys.

137

139

View full text Add to dashboard Cite

The majority of studies of the living cell rely on capturing images using fluorescence microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution in the optical microscope: structural and molecular details much finer than about half the wavelength of visible light (~200 nm) could not be visualized, imposing significant limitations on this otherwise so promising method. The surpassing of this resolution limit in far-field microscopy is currently one of the most momentous developments for studying the living cell, as the move from microscopy to super-resolution microscopy or 'nanoscopy' offers opportunities to study problems in biophysical and biomedical research at a new level of detail. This review describes the principles and modalities of present fluorescence nanoscopes, as well as their potential for biophysical and cellular experiments. All the existing nanoscopy variants separate neighboring features by transiently preparing their fluorescent molecules in states of different emission characteristics in order to make the features discernible. Usually these are fluorescent 'on' and 'off' states causing the adjacent molecules to emit sequentially in time. Each of the variants can in principle reach molecular spatial resolution and has its own advantages and disadvantages. Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels. Similar to conventional far-field microscopy, nanoscopy can be utilized for dynamical, multi-color and three-dimensional imaging of fixed and live cells, tissues or organisms. Lens-based fluorescence nanoscopy is poised for a high impact on future developments in the life sciences, with the potential to help solve long-standing quests in different areas of scientific research.

show abstract

Section: Nanoscale Writingmentioning

confidence: 99%

Lens-based fluorescence nanoscopy

Eggeling

Willig

Sahl

et al. 2015

Quart. Rev. Biophys.

137

139

View full text Add to dashboard Cite

show abstract

“…Only ~10% of all STEDlithographically prepared nanoanchors were occupied by at least a single streptavidin as compared to 98% in the case of antibodies [15]. The value is independent from the substrate passivation either by PEG or lipids.…”

Section: Discussionmentioning

confidence: 93%

“…This result was rather unexpected since we were able to load 98% of STED-lithographically fabricated nanoanchors with antibodies in a previous study [15];…”

Section: Streptavidin Loading Of Nanodotsmentioning

confidence: 85%

“…Recently, we were able to show that STEDlithographically fabricated polymer nanoanchors with properly adjusted chemical properties have the ability to bind a single antibody [15]. To extend the versatility of the polymer structures, streptavidin has been used for coating this time.…”

Section: Discussionmentioning

confidence: 99%

“…This photoresist was shown in a previous study to have antibody affinity [15]. For fabrication of nanodots with a diameter of ~70 nm (surface areas < 10 4 nm2), we used STEDlithography with 5.5 mW excitation and 28.8 mW depletion power.…”

Section: Lithography Of Polymer Nanodotsmentioning

confidence: 99%

See 2 more Smart Citations

Streptavidin functionalized polymer nanodots fabricated by visible light lithography

Wolfesberger¹,

Wollhofen²,

Buchegger³

et al. 2016

Nano Online

Self Cite

View full text Add to dashboard Cite

Exploring the Mechanisms in STED‐Enhanced Direct Laser Writing

Fischer

Mueller

Quick

et al. 2014

Advanced Optical Materials

View full text Add to dashboard Cite

Mode properties of circularly symmetric waveguides with one special type of bianisotropy are studied using finite element approach. We find that the polarization degeneracy in circularly symmetric waveguides can be eliminated, by introducing intrinsic crossing coupling between electric and magnetic moments in the constituent units of the waveguide media. Breaking the polarization degeneracy in high order mode groups is also confirmed numerically. With the bianisotropic parameters chosen in this work, the x and y-polarized modes remain decoupled. Typically, the y-polarized modes remain completely unchanged, while the x-polarized modes are turned into leaky modes that are lossy along propagation direction. A perturbation model from coupled mode theory is developed to explain the results and shows excellent agreement. Such asymmetric behavior between different polarizations might be feasible and useful for developing compact polarizers in terahertz or mid-infrared regime.

show abstract

Nano-Anchors with Single Protein Capacity Produced with STED Lithography

Cited by 44 publications

References 49 publications

Lens-based fluorescence nanoscopy

Lens-based fluorescence nanoscopy

Streptavidin functionalized polymer nanodots fabricated by visible light lithography

Exploring the Mechanisms in STED‐Enhanced Direct Laser Writing

Contact Info

Product

Resources

About