Nano Air Seeds Trapped in Mesoporous Janus Nanoparticles Facilitate Cavitation and Enhance Ultrasound Imaging

Tamarov, Konstantin; Sviridov, Andrey; Xu, Wujun; Malo, Markus; Андреев, В. Г.; Timoshenko, V.Yu.; Lehto, Vesa‐Pekka

doi:10.1021/acsami.7b11007

Cited by 28 publications

(44 citation statements)

References 63 publications

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“…37 Another explanation is concerning the presence of air trapped in the coupling medium, which can lower the cavitation threshold and act as nuclei for acoustic microbubble growth. 58,59 The high cavitational activity generated by the hydrogel LFU irradiation and the formation of a large area of LTRs did not result, however, in a greater increase in skin penetration of ZnPc. It can be seen in Table 5 that the pretreatment with the LFU-hydrogel was the one that put the lowest total amount of ZnPc in the skin.…”

Section: Dovepressmentioning

confidence: 94%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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Purpose: Sonodynamic therapy (SDT) is a new therapeutic modality for the noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Topical SDT requires the development of delivery systems to properly transport the sonosensitizer, such as zinc phthalocyanine (ZnPc), to the skin. In addition, the delivery system itself can participate in sonodynamic events and influence the therapeutic response. This study aimed to develop ZnPc-loaded micelle to evaluate its potential as a topical delivery system and as a cavitational agent for low-frequency ultrasound (LFU) application with the dual purpose of promoting ZnPc skin penetration and generating reactive oxygen species (ROS) for SDT. Methods: ZnPc-loaded micelles were developed by the thin-film hydration method and optimized using the Quality by Design approach. Micelles' influence on LFU-induced cavitation activity was measured by potassium iodide dosimeter and aluminum foil pits experiments. In vitro skin penetration of ZnPc was assessed after pretreatment of the skin with LFU and simultaneous LFU treatment using ZnPc-loaded micelles as coupling media followed by 6 h of passive permeation of ZnPc-loaded micelles. The singlet oxygen generation by LFU irradiation of the micelles was evaluated using two different hydrophilic probes. The lipid peroxidation of the skin was estimated using the malondialdehyde assay after skin treatment with simultaneous LFU using ZnPc-loaded micelles. The viability of the B16F10 melanoma cell line was evaluated using resazurin after treatment with different concentrations of ZnPc-loaded micelles irradiated or not with LFU. Results: The micelles increased the solubility of ZnPc and augmented the LFU-induced cavitation activity in two times compared to water. After 6 h ZnPc-loaded micelles skin permeation, simultaneous LFU treatment increased the amount of ZnPc in the dermis by more than 40 times, when compared to non-LFU-mediated treatment, and by almost 5 times, when compared to LFU pretreatment protocol. The LFU irradiation of micelles induced the generation of singlet oxygen, and the lipoperoxidation of the skin treated with the simultaneous LFU was enhanced in three times in comparison to the non-LFU-treated skin. A significant reduction in cell viability following treatment with ZnPc-loaded micelles and LFU was observed compared to blank micelles and non-LFU-treated control groups. Conclusion: LFU-irradiated mice can be a potential approach to skin cancer treatment by combining the functions of increasing drug penetration and ROS generation required for SDT.

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Section: Dovepressmentioning

confidence: 94%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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“…However, the microbubbles should be loaded with the gene drugs prior to the UTMD. Currently, the ultrasound microbubbles are not efficient for gene loading and poor for long time preservation, restricting the potential application (Mullin et al., 2011; Tzu-Yin et al., 2013; Ma et al., 2017; Tamarov et al., 2017). In addition, the endocytosis efficiency is relatively low after the nucleic acids are released from the damaged microbubbles (Lee et al., 2012; Duan & Lam, 2013; Zhou et al., 2016).…”

Section: Introductionmentioning

confidence: 99%

Efficient exosome delivery in refractory tissues assisted by ultrasound-targeted microbubble destruction

Sun

Zhou

et al. 2019

Drug Delivery

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Recently, exosomes have been emerged as promising drug delivery carriers, while certain tissues are intrinsically resistant to exosomes. Therapeutically improving the drug delivery efficiency in these tissues/organs would certainly broaden the potential application of exosomes in future. Ultrasound-targeted microbubble destruction (UTMD) is a promising technique for non-invasive, targeted drug delivery. In this study, we explore the possibility that UTMD assists exosome delivery in these intrinsically resistant tissues. Mice were subjected to tail vein injection of DiR-labeled exosomes together with/without UTMD of SonoVueTM, followed by in vivo and ex vivo tracking of the exosomes. As expected, heart, adipose tissue, and skeletal muscle were found reluctant to exosomes from different origins. Targeted destruction of the ultrasound microbubbles (SonoVueTM) in the heart and adipose tissue region significantly increased the exosome infiltration and endocytosis there, as revealed by fluorescence imaging and confocal laser scanning microscope (CLSM). UTMD treatment 1 h prior to exosome injection failed to facilitate the exosome endocytosis in the targeted region, indicating that the transient promoting effects of UTMD. Moreover, increases of UTMD (numerous pulses) did not linearly enhance the exosome delivery. Together, our study here has established a novel strategy for targeted delivery of exosomes in the reluctant tissues, by combining the advantages of ultrasound microbubbles and exosomes in drug delivery.

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“…In order to prepare J-PSi NPs, hexane was used as a nano-stopper (Xu et al, 2014; Tamarov et al, 2017) to protect hydrogen terminated (hydrophobic) pore walls that were the result of etching. Second, the unprotected outer surface was selectively oxidized in NH 4 OH:H 2 O 2 (30%):H 2 O 1:1:6 at RT for 15 min and in HCl:H 2 O 2 (30%):H 2 O 1:1:6 for 15 min, subsequently.…”

Section: Methodsmentioning

confidence: 99%

“…In our recent study, Janus-like PSi NPs were designed to be specifically employed with ultrasound. In these NPs, the pore walls were hydrophobic and the external surfaces of NPs were hydrophilic giving sustainability in aqueous solution while maintaining air inside the pores (Tamarov et al, 2017). The present work compares the enhanced heating effects produced by fully oxidized, hydrophilic PSi NPs (O-PSi NPs), and Janus-like PSi NPs (J-PSi NPs) in the field of the US traveling wave, and it explains the phenomenology behind the exceptional cavitation and heating capability of these NPs.…”

Section: Introductionmentioning

confidence: 99%

Cavitation Induced by Janus-Like Mesoporous Silicon Nanoparticles Enhances Ultrasound Hyperthermia

et al. 2019

Self Cite

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The presence of nanoparticles lowers the levels of ultrasound (US) intensity needed to achieve the therapeutic effect and improves the contrast between healthy and pathological tissues. Here, we evaluate the role of two main mechanisms that contribute to the US-induced heating of aqueous suspensions of biodegradable nanoparticles (NPs) of mesoporous silicon prepared by electrochemical etching of heavily boron-doped crystalline silicon wafers in a hydrofluoric acid solution. The first mechanism is associated with an increase of the attenuation of US in the presence of NPs due to additional scattering and viscous dissipation, which was numerically simulated and compared to the experimental data. The second mechanism is caused by acoustic cavitation leading to intense bubble collapse and energy release in the vicinity of NPs. This effect is found to be pronounced for as-called Janus NPs produced via a nano-stopper technique, which allow us to prepare mesoporous NPs with hydrophobic inner pore walls and hydrophilic external surface. Such Janus-like NPs trap air inside the pores when dispersed in water. The precise measurement of the heating dynamics in situ enabled us to detect the excessive heat production by Janus-like NPs over their completely hydrophilic counterparts. The excessive heat is attributed to the high intensity cavitation in the suspension of Janus-like NPs. The present work elicits the potential of specifically designed Janus-like mesoporous silicon NPs in the field of nanotheranostics based on ultrasound radiation.

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Nano Air Seeds Trapped in Mesoporous Janus Nanoparticles Facilitate Cavitation and Enhance Ultrasound Imaging

Cited by 28 publications

References 63 publications

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Efficient exosome delivery in refractory tissues assisted by ultrasound-targeted microbubble destruction

Cavitation Induced by Janus-Like Mesoporous Silicon Nanoparticles Enhances Ultrasound Hyperthermia

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