Nano 3D printing-enabled micropost array gradients

Mengis, Aaman; Gopal, Riddhi; Feldman, Naomi H.; Kedia, Nikita; Hesley, David; Young, J. L.; Fobe, Theodore L.; Fernández, Luis Sánchez; Anderson, Christine; Sochol, Ryan D.

doi:10.1109/memsys.2017.7863441

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…The theoretical calculation is consistent with the FEM results. However, these results were several times higher than the reported force by HUVECs, 44,45 approximately 400 nN. The micropillar displacement and thus the force we measured might mainly be attributed to the shrinking of cells during gradient dehydration by ethanol rather than the actual traction force exerted by live HUVECs.…”

Section: Calculation Of Traction Force To Micropillars Exerted By Huvecsmentioning

confidence: 99%

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Wang

Liu

Gao

et al. 2023

ACS Appl. Mater. Interfaces

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Surface topography is a biophysical factor affecting cell behaviors, yet the underlying cues are still not clear. Herein, we hypothesized that stereo coverage and overall stiffness of biomaterial arrays on the scale of single cells underly parts of topography effects on cell adhesion. We fabricated a series of microarrays (micropillar, micropit, and microtube) of poly(L-lactic acid) (PLLA) using mold casting based on pre-designed templates. The characteristic sizes of array units were less than that of a single cell, and thus, each cell could sense the micropatterns with varied roughness. With human umbilical vein endothelial cells (HUVECs) as the model cell type, we examined spreading areas and cell viabilities on different surfaces. "Stereo coverage" was defined to quantify the actual cell spreading fraction on a topographic surface. Particularly in the case of high micropillars, cells were confirmed not able to touch the bottom and had to partially hang among the micropillars. Then, in our opinion, a cell sensed the overall stiffness combining the bulk stiffness of the raw material and the stiffness of the culture medium. Spreading area and single cell viability were correlated to coverage and topographic feature of the prepared microarrays in particular with the significantly protruded geometry feather. Cell traction forces exerted on micropillars were also discussed. These findings provide new insights into the surface modifications toward biomedical implants.

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Section: Calculation Of Traction Force To Micropillars Exerted By Huvecsmentioning

confidence: 99%

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Wang

Liu

Gao

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

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Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review

et al. 2023

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Sensing the mechanical properties of the substrates or the matrix by the cells and the tissues, the subsequent downstream responses at the cellular, nuclear and epigenetic levels and the outcomes are beginning to get unraveled more recently. There have been various instances where researchers have established the underlying connection between the cellular mechanosignalling pathways and cellular physiology, cellular differentiation, and also tissue pathology. It has been now accepted that mechanosignalling, alone or in combination with classical pathways, could play a significant role in fate determination, development, and organization of cells and tissues. Furthermore, as mechanobiology is gaining traction, so do the various techniques to ponder and gain insights into the still unraveled pathways. This review would briefly discuss some of the interesting works wherein it has been shown that specific alteration of the mechanical properties of the substrates would lead to fate determination of stem cells into various differentiated cells such as osteoblasts, adipocytes, tenocytes, cardiomyocytes, and neurons, and how these properties are being utilized for the development of organoids. This review would also cover various techniques that have been developed and employed to explore the effects of mechanosignalling, including imaging of mechanosensing proteins, atomic force microscopy (AFM), quartz crystal microbalance with dissipation measurements (QCMD), traction force microscopy (TFM), microdevice arrays, Spatio-temporal image analysis, optical tweezer force measurements, mechanoscanning ion conductance microscopy (mSICM), acoustofluidic interferometric device (AID) and so forth. This review would provide insights to the researchers who work on exploiting various mechanical properties of substrates to control the cellular and tissue functions for tissue engineering and regenerative applications, and also will shed light on the advancements of various techniques that could be utilized to unravel the unknown in the field of cellular mechanobiology. Graphical Abstract

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Nano 3D printing-enabled micropost array gradients

Cited by 2 publications

References 19 publications

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Stereo Coverage and Overall Stiffness of Biomaterial Arrays Underly Parts of Topography Effects on Cell Adhesion

Trends in mechanobiology guided tissue engineering and tools to study cell-substrate interactions: a brief review

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