Nannochloropsis oculata enhances immune response, transcription of stress, and cytokine genes in Nile tilapia subjected to air exposure stress

Zahran, Eman; Elbahnaswy, Samia; Ibrahim, Iman; Khaled, Asmaa A.

doi:10.1016/j.aqrep.2021.100911

Cited by 11 publications

(19 citation statements)

References 78 publications

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“…However, the chemical composition, mainly the carotenoid type and content, and the microalgae incorporation level will determine the effect on each fish species. Dietary algal inclusion in Nile Tilapia diets did not impose any nutritional stress that supports the liver function enzymes results herein and is also evidenced histopathologically in Zahran et al (2021). Our results coincide with Batista et al (2020) and Peixoto et al (2016), who reported no adverse effect on oxidant or antioxidant enzyme activities in European Seabass fed Gracilaria gracilis, N. oceanica, single or blended, and seaweed-supplemented diets.…”

Section: Discussionsupporting

confidence: 90%

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Salem

Adawy²,

Zaki

et al. 2022

J Aqua Anim Hlth

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Objective:The current study evaluated the potential roles of incorporating Nannochloropsis oculata into the diet of Nile Tilapia Oreochromis niloticus in an 8week trial. Methods: Dietary supplementation of N. oculata was tested at inclusion levels (0%[control], 5% [N5], and 10% [N1]) in triplicate. After the trial, comprehensive fish health indicators were evaluated.Result: N. oculata-supplemented feed had a stimulatory effect on fish body weight, where a significant increase in final weight and specific growth rate was observed in the N10 group compared to the control. Better feed conversion was observed at N5 and N10 compared to control. Organosomatic indices were elevated significantly in the N5 group compared to the N10 and control groups. Serum lysozyme activity was significantly increased in the N10 group compared to N5 and control groups. Levels of IgM were significantly higher in N10 compared to the control and N5 groups, with no significance between the latter. Amylase activity showed a significant enhancement in N10 compared to N5. Both levels of N. oculata preserved hepatic health and antioxidant status. Light and transmission electron microscopy showed that Nile Tilapia fed N. oculata at both levels enhanced intestinal immunity, integrity, and absorptive efficiency. The protecting effect of N. oculata was confirmed against Aeromonas hydrophila challenge, where cumulative mortalities were significantly decreased in N5 and N10 groups compared with the control and more in N10. Conclusion:This work confirmed the different beneficial roles of N. oculata dietary supplementation for a Nile Tilapia balanced diet.

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Section: Discussionsupporting

confidence: 90%

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Salem

Adawy²,

Zaki

et al. 2022

J Aqua Anim Hlth

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“…Additionally, immunoglobulins have very important roles in the defense mechanism via killing microbes and pathogens and restricting the spread of infectious agents [ 18 ]. Herein, our consequences cleared that using various levels of NSS microalga mixture enhanced the Nile tilapia immune system (IgM, lysozymes, and MPO) prior to the challenge as previously declared elsewhere [ 30 , 98 ]. Similarly, dietary supplementation with microalgae blends comprising Schizochytrium species, Spirulina platensis , Chloroella sorokiniana , and Chromochloris zofingiensis significantly decreased the genes expression of pro-inflammatory cytokines; IL-8, IL-6, and IL-1β and increased the lysozyme activity in zebra fish [ 99 ].…”

Section: Discussionsupporting

confidence: 69%

“…This study denoted that Nile tilapia groups fed NSS microalgae blends supplemented diet showed HSP70 low expression levels. In accordance, the expression levels of HSP70 were downregulated with increasing levels of Nannochloropsis oculate in Nile tilapia subjected to air stress [ 30 ].…”

Section: Discussionmentioning

confidence: 92%

“…The feeding of sea bream ( Sparidentex hasta ) on supplemental DHA could enhance the serum immunological parameters like lysozyme and phagocytic activity and modulated the expression of IL - 1B , IL - 6 , and IL - 10 genes [ 107 ]. Furthermore, Nile tilapia fed 10% Nannochloropsis oculata exhibited significant upregulation of TGF - β and IL - 10 and marked downregulation of IL - 1β and TNF - α genes [ 30 ]. In this regard, the anti-inflammatory properties of Nannochloropsis oculata could be attributed to its role as a good potential source of EPA and its high contents of pigments such as zeaxanthin, chlorophyll, astaxanthin, and canthaxanthin [ 108 ].…”

Section: Discussionmentioning

confidence: 99%

“…Nannochloropsis oculata ( N. oculata ), an eukaryotic unicellular microalga, is broadly used in aquaculture industry with an important nutritional value due to its elevated contents of proteins and PUFAs, particularly EPA [ 26 ]. The feeding of fish on N. oculata supplemented diets has possibly been considered to improve the growth performance, feed utilization, immune response, anti-inflammatory activity, antioxidant capacity, and resistance against pathogenic bacterial species [ 27 , 28 , 29 ] and mitigate the oxidative stress [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Impacts of Fortifying Nile Tilapia (Oreochromis niloticus) Diet with Different Strains of Microalgae on Its Performance, Fillet Quality and Disease Resistance to Aeromonas hydrophila Considering the Interplay between Antioxidant and Inflammatory Response

et al. 2022

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The oxidative stress facing fish during intensive production brings about diseases and mortalities that negatively influence their performance. Along with that, the increased awareness of omega-3 polyunsaturated fatty acids (omega-3-PUFAs) health benefits has been triggered the introduction of alternative additives in aqua feed that cause not only modulation in fish immune response but also fortification of their fillet. In this context, the role of microalgae mix (NSS) containing Nannochloropsis oculate and Schizochytrium and Spirulina species, which were enriched with bioactive molecules, especially EPA and DHA, was assessed on Nile tilapia’s performance, fillet antioxidant stability, immune response, and disease resistance. Varying levels of NSS (0.75, 1.5, and 3%) were added to Nile tilapia’s diet for 12 weeks and then a challenge of fish with virulent Aeromonas hydrophila (A. hydrophila) was carried out. Results showed that groups fed NSS, especially at higher levels, showed an improved WG and FCR, which corresponded with enhanced digestive enzymes’ activities. Higher T-AOC was detected in muscle tissues of NSS3.0% fed fish with remarkable reduction in ROS, H2O2, and MDA contents, which came in parallel with upregulation of GSH-Px, CAT, and SOD genes. Notably, the contents of EPA and DHA in fillet were significantly increased with increasing the NSS levels. The mean log10 counts of pathogenic Vibrio and Staphylococcus species were reduced, and conversely, the populations of beneficial Lactobacillus and Bacillus species were increased more eminent after supplementation of NSS3.0% and NSS1.5%. Moreover, regulation of the immune response (lysozyme, IgM, ACH50, NO, and MPO), upregulation of IL-10, TGF-β, and IgM, and downregulation of IL-1β, TNF-α, HSP70, and COX-2 were observed following dietary higher NSS levels. After challenge, reduction in A. hydrophila counts was more prominent, especially in NSS3.0% supplemented group. Taken together, the current study encourages the incorporation of such microalgae mix in Nile tilapia’s diet for targeting maximum performance, superior fillet quality, and protection against A. hydrophila.

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Influence of astaxanthin-enriched Haematococcus pluvialis microalgae on the growth efficacy, immune response, antioxidant capacity, proinflammatory cytokines, and tissue histomorphology of hybrid red tilapia

Eldessouki,

Elshopakey,

Elbahnaswy

et al. 2024

Aquacult Int

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Nannochloropsis oculata enhances immune response, transcription of stress, and cytokine genes in Nile tilapia subjected to air exposure stress

Cited by 11 publications

References 78 publications

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Nannochloropsis oculata supplementation improves growth, immune response, intestinal integrity, and disease resistance of Nile Tilapia

Impacts of Fortifying Nile Tilapia (Oreochromis niloticus) Diet with Different Strains of Microalgae on Its Performance, Fillet Quality and Disease Resistance to Aeromonas hydrophila Considering the Interplay between Antioxidant and Inflammatory Response

Influence of astaxanthin-enriched Haematococcus pluvialis microalgae on the growth efficacy, immune response, antioxidant capacity, proinflammatory cytokines, and tissue histomorphology of hybrid red tilapia

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