NAM-1gene polymorphism and grain protein content in Hordeum

Jamar, Catherine; Loffet, Francois; Frettinger, Patrick; Ramsay, Luke; Fauconnier, Marie‐Laure; Jardin, Patrick du

doi:10.1016/j.jplph.2009.10.014

Cited by 29 publications

(35 citation statements)

References 27 publications

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“…There have been only a few detailed studies on NAC genes from species with non-sequenced genomes, such as wheat and barley [11,12]. …”

Section: Introductionmentioning

confidence: 99%

“…Finally, there have been two publications on the barley HvNAM-1 [12,46] encoding a homologue of the wheat TtNAM-B1 NAC transcription factor shown to regulate both senescence and grain protein content (GPC) [11]. Gene polymorphisms of HvNAM-1 in three Hordeum species explained some of the variation in GPC [12] suggesting that the biological function of HvNAM-1 is similar to that of TtNAM-B1.…”

Section: Introductionmentioning

confidence: 99%

“…Gene polymorphisms of HvNAM-1 in three Hordeum species explained some of the variation in GPC [12] suggesting that the biological function of HvNAM-1 is similar to that of TtNAM-B1.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of barley (Hordeum vulgare L.) NAC transcription factors suggests conserved functions compared to both monocots and dicots

2011

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BackgroundThe NAC transcription factor family is involved in the regulation of traits in both monocots and dicots of high agronomic importance. Understanding the precise functions of the NAC genes can be of utmost importance for the improvement of cereal crop plants through plant breeding. For the cereal crop plant barley (Hordeum vulgare L.) only a few NAC genes have so far been investigated.ResultsThrough searches in publicly available barley sequence databases we have obtained a list of 48 barley NAC genes (HvNACs) with 43 of them representing full-length coding sequences. Phylogenetic comparisons to Brachypodium, rice, and Arabidopsis NAC proteins indicate that the barley NAC family includes members from all of the eight NAC subfamilies, although by comparison to these species a number of HvNACs still remains to be identified. Using qRT-PCR we investigated the expression profiles of 46 HvNACs across eight barley tissues (young flag leaf, senescing flag leaf, young ear, old ear, milk grain, late dough grain, roots, and developing stem) and two hormone treatments (abscisic acid and methyl jasmonate).ConclusionsComparisons of expression profiles of selected barley NAC genes with the published functions of closely related NAC genes from other plant species, including both monocots and dicots, suggest conserved functions in the areas of secondary cell wall biosynthesis, leaf senescence, root development, seed development, and hormone regulated stress responses.

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“…There have been only a few detailed studies on NAC genes from species with non-sequenced genomes, such as wheat and barley [11,12]. …”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of barley (Hordeum vulgare L.) NAC transcription factors suggests conserved functions compared to both monocots and dicots

2011

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“…Multiple alignments of protein sequences were performed with the program Clustal X version 2.0 program (Larkin et al, 2007). The phylogenetic comparison of isolated full-length TaNAC1 and characterized NAC proteins based on the literature (Ooka et al, 2003; Jamar et al, 2010; Christiansen et al, 2011; D’haeseleer et al, 2011; Tang et al, 2012; Feng et al, 2014) and GenBank 5 were constructed with the neighbor-joining algorithm using MEGA4 program (Tamura et al, 2007). Bootstrap values were calculated from 1000 bootstrap replicates.…”

Section: Methodsmentioning

confidence: 99%

TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana

et al. 2015

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Plant-specific NAC transcription factors (TFs) constitute a large family and play important roles in regulating plant developmental processes and responses to environmental stresses, but only some of them have been investigated for effects on disease reaction in cereal crops. Virus-induced gene silencing (VIGS) is an effective strategy for rapid functional analysis of genes in plant tissues. In this study, TaNAC1, encoding a new member of the NAC1 subgroup, was cloned from bread wheat and characterized. It is a TF localized in the cell nucleus, and contains an activation domain in its C-terminal. TaNAC1 was strongly expressed in wheat roots and was involved in responses to infection by the obligate pathogen Puccinia striiformis f. sp. tritici and defense-related hormone treatments such as salicylic acid (SA), methyl jasmonate, and ethylene. Knockdown of TaNAC1 with barley stripe mosaic virus-induced gene silencing (BSMV-VIGS) enhanced stripe rust resistance. TaNAC1-overexpression in Arabidopsis thaliana plants gave enhanced susceptibility, attenuated systemic-acquired resistance to Pseudomonas syringae DC3000, and promoted lateral root development. Jasmonic acid-signaling pathway genes PDF1.2 and ORA59 were constitutively expressed in transgenic plants. TaNAC1 overexpression suppressed the expression levels of resistance-related genes PR1 and PR2 involved in SA signaling and AtWRKY70, which functions as a connection node between the JA- and SA-signaling pathways. Collectively, TaNAC1 is a novel NAC member of the NAC1 subgroup, negatively regulates plant disease resistance, and may modulate plant JA- and SA-signaling defense cascades.

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“…The resulting RNAi plants display a pronounced delay in the decay of chlorophyll content. Although the original identification took place in wheat plants, it was next shown that in barley, a species closely related to wheat, homologous genes also contribute to accelerated senescence (Distelfeld et al, 2008;Jukanti et al 2008;Jamar et al, 2010;Cai et al, 2013). Interestingly, identification and characterization of the closest homologue in rice suggested that conservation of the functional role is in fact restricted to some Poaceae groups indicating a dynamic history of NAC transcription factors in grasses (Distelfeld et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

Checovich

Galatro

Moriconi

et al. 2016

Plant Physiology and Biochemistry

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SummaryTaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity. 3 Highlights•TaNAM-RNAi stay-green phenotype is coupled with maintenance of chloroplast structure.•That stay-green phenotype is associated with a high enzymatic antioxidant activity.•Chloroplast dismantling correlates with decay of iron and protein content in leaves.

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NAM-1gene polymorphism and grain protein content in Hordeum

Cited by 29 publications

References 27 publications

Characterization of barley (Hordeum vulgare L.) NAC transcription factors suggests conserved functions compared to both monocots and dicots

Characterization of barley (Hordeum vulgare L.) NAC transcription factors suggests conserved functions compared to both monocots and dicots

TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana

The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity

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