Nakamurella flava sp. nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.

Abstract

A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq. collected from Guizhou, PR China and tested by a polyphasic approach to determine its taxonomic position. Strain N5BH11T grew optimally at 30 °C, pH 6.0–7.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %). The DNA G+C content of strain N5BH11T was 71.6 mol%. The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis , Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively. The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis , N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c. On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella , for which the name Nakamurella flava sp. nov. is proposed. The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).

Tardigrade Community Microbiomes in North American Orchards Include Putative Endosymbionts and Plant Pathogens

“…Nakamurella intestinalis has been isolated from the feces of another Ecdysozoan, the katydid Pseudorynchus japonicus ( Kim et al, 2017 ). Nakamurella endophytica and N. flava were identified as endophytes of mangroves and mint, respectively ( Tuo et al, 2016 ; Yan et al, 2020 ), and N. albus and N. leprariae were originally discovered in lichens ( Jiang et al, 2020 ; An et al, 2021 ). This suggests that tardigrades could obtain endophytic or lichen-dwelling Nakamurella from their habitat.…”

Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts

“…Actinomycetes of other genera can also be isolated from medicinal plants, but the number is relatively small compared with Streptomyces. Many studies have isolated new species of bioactive endophytic actinomycetes from medicinal plants, which greatly expanded microbial resources and laid a foundation for the industrial application of actinomycetes [ 52 , 53 , 58 , 60 , 61 ]. The results of endophyte isolation from medicinal plants in recent years are shown in Table 3 .…”

Tardigrade community microbiomes in North American orchards include putative endosymbionts and plant pathogens

“…flava were identified as endophytes of mangroves and mint, respectively (Yan et al 2020;Tuo et al 2016), and N. albus and N. leprariae were originally discovered in lichens (Jiang et al 2020;An et al 2021). This suggests that tardigrades could obtain endophytic or lichen-dwelling Nakamurella from their habitat.…”