Naïve-like conversion of bovine induced pluripotent stem cells from Sertoli cells

Jiang, Yu; Cai, Ningning; An, Xing-Lan; Zhu, Wenyao; Yang, Rui; Tang, Bo; Li, Ziyi; Zhang, Xueming

doi:10.1016/j.theriogenology.2022.10.043

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…Our work showed that using a bovine embryonic fibroblast feeder layer enhances the reprogramming of bovine somatic cells, and thus is more conducive to the long-term maintenance of the colony morphology and pluripotency than using an MEF feeder layer. This method also improves the efficiency of the conversion of primed biPSC-like cells to naïve biPSC-like cells [39]. Successful conversion systems of primed iPSC-like cells to naïve iPSC-like cells in other species including humans provide more lessons for cattle [62,63].…”

Section: Culture Systemmentioning

confidence: 95%

“…In 2017, single-cell-derived biPSC-like cells that can be stably passaged for more than 50 generations were obtained, which can express essential pluripotency genes and form embryoid bodies and teratomas [37]. We also derived biPSC-like cells from bovine immature Sertoli cells recently [38,39]. Generally, the main factors affecting the quality of biPSCs include vectors, transcription factor combinations, culture systems, and somatic cell sources.…”

Section: Establishment Of Bovine Ipscs (Bipscs)mentioning

confidence: 99%

“…Moreover, the differentiation status of the cells is a central factor in reprogramming efficiency, and progenitor cells are more likely to be reprogrammed than differentiated terminal cells [65]. biPSC-like cells have been derived from somatic cells such as embryonic fibroblasts, amniotic cells, mammary cells, and Sertoli cells [38,39]. These reports showed that epigenetic disorders in bovine fibroblasts prevented complete reprogramming.…”

Section: Source Cellsmentioning

confidence: 99%

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Bovine Pluripotent Stem Cells: Current Status and Prospects

Chen,

Tang,

Xie

et al. 2024

IJMS

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Pluripotent stem cells (PSCs) can differentiate into three germ layers and diverse autologous cell lines. Since cattle are the most commonly used large domesticated animals, an important food source, and bioreactors, great efforts have been made to establish bovine PSCs (bPSCs). bPSCs have great potential in bovine breeding and reproduction, modeling in vitro differentiation, imitating cancer development, and modeling diseases. Currently, bPSCs mainly include bovine embryonic stem cells (bESCs), bovine induced pluripotent stem cells (biPSCs), and bovine expanded potential stem cells (bEPSCs). Establishing stable bPSCs in vitro is a critical scientific challenge, and researchers have made numerous efforts to this end. In this review, the category of PSC pluripotency; the establishment of bESCs, biPSCs, and bEPSCs and its challenges; and the application outlook of bPSCs are discussed, aiming to provide references for future research.

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Section: Culture Systemmentioning

confidence: 95%

Section: Establishment Of Bovine Ipscs (Bipscs)mentioning

confidence: 99%

Section: Source Cellsmentioning

confidence: 99%

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Bovine Pluripotent Stem Cells: Current Status and Prospects

Chen,

Tang,

Xie

et al. 2024

IJMS

Self Cite

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“…Despite its unquestionable potential in the fields of agriculture and biotechnology, iPSC research has still not received the deserved attention. Approximately thousands of studies are currently focusing on murine and human cell reprogramming, and we could find many studies that described cell reprogramming in important mammalian farm animals [ 24 , 89 , 94 , 97 , 101 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 ]. We propose that future studies should focus on the agricultural and biopharmaceutical applications of the iPSC technology.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

Verma¹,

Lee

Salamone

2022

Animals

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Induced pluripotent stem cell (iPSC) technology is an emerging technique to reprogram somatic cells into iPSCs that have revolutionary benefits in the fields of drug discovery, cellular therapy, and personalized medicine. However, these applications are just the tip of an iceberg. Recently, iPSC technology has been shown to be useful in not only conserving the endangered species, but also the revival of extinct species. With increasing consumer reliance on animal products, combined with an ever-growing population, there is a necessity to develop alternative approaches to conventional farming practices. One such approach involves the development of domestic farm animal iPSCs. This approach provides several benefits in the form of reduced animal death, pasture degradation, water consumption, and greenhouse gas emissions. Hence, it is essentially an environmentally-friendly alternative to conventional farming. Additionally, this approach ensures decreased zoonotic outbreaks and a constant food supply. Here, we discuss the iPSC technology in the form of a “Frozen Ark”, along with its potential impact on spreading awareness of factory farming, foodborne disease, and the ecological footprint of the meat industry.

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“…To date, however, blastoids from other species have not been reported. Recently, several types of pluripotent stem cells (PSCs), including EPSCs, have been derived from Bos taurus blastocysts [9][10][11][12][13][14][15] , which hold great potential to advance animal agriculture 16 . Surprisingly, we found a bovine EPSC condition 13,17 could support de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs) (Wang et al, manuscript co-submitted).…”

mentioning

confidence: 99%

Bovine blastocyst like structures derived from stem cell cultures

Pinzón-Arteaga

Wang

Wei

et al. 2022

Preprint

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Understanding blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. We developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via the assembly of trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, and represent an accessible in vitro model for studying bovine embryogenesis.

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Naïve-like conversion of bovine induced pluripotent stem cells from Sertoli cells

Cited by 7 publications

References 28 publications

Bovine Pluripotent Stem Cells: Current Status and Prospects

Bovine Pluripotent Stem Cells: Current Status and Prospects

iPSC Technology: An Innovative Tool for Developing Clean Meat, Livestock, and Frozen Ark

Bovine blastocyst like structures derived from stem cell cultures

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