Naïve B cells with low differentiation improve the immune reconstitution of HIV-infected patients

Jia, Jie; Zhao, Yu; Yang, Ji-Qun; Lu, Dan; Zhang, Xiuling; Mao, Jun-Hong; Wang, Kunhua; Wang, Jianhua; Kuang, Yi‐Qun

doi:10.1016/j.isci.2022.105559

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…This may be related to the dysregulation of memory B cells and circulating T follicular helper cells ( 149 , 150 ). In addition, another study found that the diversity of the B-cell receptor repertoire in HIV-INRs was decreased, and naïve B cells with low differentiation improve the immune reconstitution ( 151 ). These findings underscore the critical role of B cells in immune reconstitution after HIV infection.…”

Section: Other Immune Cellsmentioning

confidence: 99%

Cellular and molecular insights into incomplete immune recovery in HIV/AIDS patients

Yan

Xiao

et al. 2023

Front. Immunol.

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Highly active antiretroviral therapy (ART) can effectively inhibit virus replication and restore immune function in most people living with human immunodeficiency virus (HIV). However, an important proportion of patients fail to achieve a satisfactory increase in CD4+ T cell counts. This state is called incomplete immune reconstitution or immunological nonresponse (INR). Patients with INR have an increased risk of clinical progression and higher rates of mortality. Despite widespread attention to INR, the precise mechanisms remain unclear. In this review, we will discuss the alterations in the quantity and quality of CD4+ T as well as multiple immunocytes, changes in soluble molecules and cytokines, and their relationship with INR, aimed to provide cellular and molecular insights into incomplete immune reconstitution.

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Section: Other Immune Cellsmentioning

confidence: 99%

Cellular and molecular insights into incomplete immune recovery in HIV/AIDS patients

Yan

Xiao

et al. 2023

Front. Immunol.

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“…The single-cell library was constructed and sequenced, and the single-cell data were analyzed as previously described 10 .…”

Section: Library Construction Single-cell Sequencing and Data Analysismentioning

confidence: 99%

“…However, there are still 20%-40% of HIV-infected patients fail to restore the CD4 + T cell counts to normal levels (500 cells/µl) despite under optimal ART, called immunological non-responders (INRs, CD4 + T cell < 350 cells/µl), which is contrary to immunological responders (IRs, CD4 + T cell > 350 cells/µl) 6,7,8,9 . An increasing body of evidence shows that incomplete immune reconstitution weakens the immune response 8,10,11,12 , thus increasing morbidity and mortality of AIDS and non-AIDS diseases 13,14,15 . The damage of immune cell functions by pyroptosis 12 , mitochondrial dysfunction 16 , and viral replication dynamics contributes to immunological nonresponses 17,18 .…”

Section: Introductionmentioning

confidence: 99%

CD4highCD8low double-positive T cells with high differentiation mediate incomplete immune reconstitution in HIV-infected patients

Zhao

Jia

Zhang

et al. 2023

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Antiretroviral therapy (ART) is the most generally used to treat human immunodeficiency virus (HIV)-infected patients. However, some patients experience incomplete immune reconstitution and fail to restore CD4+ T cell counts after treating with ART. Herein, we investigated the characters of CD4+CD8+ double-positive (DP) T cells at the transcriptomic level by analyzing single-cell RNA sequencing of peripheral blood mononuclear cells from HIV-infected immunological non-responders (INRs) and immunological responders (IRs). We identified eight DP T cell clusters and grouped into three populations CD4highCD8low, CD4lowCD8high, and CD4lowCD8low, respectively. Decreased proportion of DP T cells was detected in INRs, including CD4highCD8low DP T cells in the process of cellular differentiation, the latter demonstrated a reduced source of DP T cells in INRs. CD4highCD8low DP T cells in IRs and INRs as a heterogenous population, were distinct on the expression of GZMA/B/H, LAG3, NKG7 and GNLY, which related to the function of cell activation and cellular differentiation, cytotoxicity and programmed cell death. These data provide a comprehensive analysis of gene signatures of CD4highCD8low DP T cells associated with failed immune reconstitution after HIV infection, which could be useful in developing new cure strategies for HIV-infected patients.

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Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

Jia¹,

Zhao²,

Wang³

et al. 2023

STAR Protocols

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Naïve B cells with low differentiation improve the immune reconstitution of HIV-infected patients

Cited by 4 publications

References 33 publications

Cellular and molecular insights into incomplete immune recovery in HIV/AIDS patients

Cellular and molecular insights into incomplete immune recovery in HIV/AIDS patients

CD4highCD8low double-positive T cells with high differentiation mediate incomplete immune reconstitution in HIV-infected patients

Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

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