Naidispora caidianensis n. gen. n. sp. infecting coelomocytes of oligochaete Branchiura sowerbyi (Oligochaeta: Naididae) in China

Liu, Xinhua; Stentiford, Grant D.; Ren, Shisi; Song, Rui; Yu, Jianbo; Li, Deliang; Ji, Xiang; Zhang, Jinyong

doi:10.1016/j.jip.2022.107768

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…They are characterized by the production of infective spores which transmit the infective sporoplasm to the host cytoplasm or nucleoplasm by a unique complicated eversion apparatus-polar tube yet exhibit a closer phylogenetic affinity to fungi [ 3 , 4 , 5 ]. Over 160 species across 22 genera have been identified in fish [ 6 , 7 ], and new species are continuously emerging [ 8 , 9 , 10 ]. Many microsporidians pose a significant threat to pisciculture.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Cultivation for Glugea plecoglossi (Microsporidia) Isolated from Ayu (Plecoglossus altivelis)

Xu,

Zhang,

Zhou

et al. 2024

Microorganisms

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Glugea plecoglossi is an obligate intracellular microsporidium, which poses a significant threat to ayu (Plecoglossus altivelis). In vitro cultivation models are invaluable tools for investigating intracellular microorganisms, including G. plecoglossil. In this study, we attempted to in vitro cultivate G. plecoglossi using primary cultures derived from ayu monocytes/macrophages (MO/MΦ), a murine-derived macrophage cell line RAW264.7, and the epithelioma papulosum cyprini (EPC) cell line. The results demonstrated that MO/MΦ infected with spores exhibited a pronounced immune response which was presented by rapidly high expression levels of inflammatory cytokines, such as PaIL-1β, PaTNF-α, PaIL-10, and PaTGF-β, and detached within 96 h post-infection (hpi). Infected RAW264.7 cells remained capable of stable passage yet exhibited cellular deformation with a decrease in intracellular spores occurring around 8 days post-infection (dpi). In contrast, EPC cells promised a substantial parasite population, and the cytokine expression levels returned to normal by 8 dpi. In addition, G. plecoglossi spores recovered from EPC cells could infect young ayu, suggesting that EPC cells might be used as an in vitro cultivation system for G. plecoglossi.

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