Nahrungsmittel und deren Hilfsstoffe

Copius‐Peereboom, J. W.

doi:10.1007/978-3-642-49187-0_22

Cited by 2 publications

(2 citation statements)

References 58 publications

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“…The samples were then concentrated by the use of a rotating evaporator, and chromatographed on precoated silica gels with the following eluent mixture: ethanol/chloroform/NH3/water (530:303:152:15, by vol. ;Copius-Peereboom, 1967). Efficiency of separation was controlled with L-lactate, pyruvate, thiolactate (2-DL-mercaptopropionate), L-alanine and propionate.…”

Section: Tlcmentioning

confidence: 99%

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Hildmann

Storelli

Haase

et al. 1980

Biochemical Journal

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Uptake of L-lactate into rabbit jejunal brush-border-membrane vesicles prepared by a Ca2+-precipitation procedure was studied by a rapid filtration technique with L-[14C]-lactate as tracer. Transport of L-lactate into an intravesicular (osmotically reactive) space could be established. An inwardly directed NaCl gradient (outside 21 mM/inside 0mM) stimulated the uptake of L-lactate at 15 s 2-4-fold compared with that observed with an equal KCl gradient. A transient accumulation of L-lactate inside the vesicles (overshoot) was observed in the presence of an NaCl gradient. Gradients of LiCl, RbCl, CsCl or choline chloride were not able to replace NaCl in the stimulation of L-lactate uptake. L-Lactate uptake was saturable only in the presence of Na+. D-Lactate, DL-thiolactate (2-DL-mercaptopropionate), pyruvate and propionate inhibited the Na+-stimulated L-lactate uptake; D-lactate, thiolactate and pyruvate provoked trans-stimulation of L-lactate uptake. Artificially imposed diffusion potentials (inside negative) did not exert any effect on the Na+-dependent L-lactate uptake. The results are consistent with the existence of an electroneutral Na+/L-lactate co-transport system in the brush border of rabbit small intestine.

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Section: Tlcmentioning

confidence: 99%

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Hildmann

Storelli

Haase

et al. 1980

Biochemical Journal

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“…Aftcr 45 min at 90°C the absorbance at 525 nm was measured against a blank prepared with a 4-M-acetic-acid eluate containing no glycolic acid. Thin-layer chromatography of glycolate was performed with pre-coated silica gel 60 F254 plates (Merck) at room temperature with ethanol/chloroform/ammonia solution (u' ;;' 0.91 )/HzO (53 : 30.3 : 15.2: 1.5), v/v [16]. Glycolic acid was detected with a 2,6-dichloroindophenol spray [I 71.…”

Section: Methodsmentioning

confidence: 99%

Production of Glycolate by Oxidation of the 1,2‐Dihydroxyethyl‐thiamin‐diphosphate Intermediate of Transketolase with Hexacyanoferrate(III) or H₂O₂

Christen

Gasser

1980

European Journal of Biochemistry

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In the presence of hexacyanoferrate(III), or other suitable oxidants, transketolase catalyzes the oxidative cleavage of its donor substrates xylulose 5-phosphate or fructose 6-phosphate into glycolate and glyceraldehyde 3-phosphate or erythrose 4-phosphate, respectively. Two moles of hexacyanoferrate ( Glycolate is also produced with H202 as oxidant; however, the reaction is at least two orders of magnitude slower than with hexacyanoferrate( 111).

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Nahrungsmittel und deren Hilfsstoffe

Cited by 2 publications

References 58 publications

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Production of Glycolate by Oxidation of the 1,2‐Dihydroxyethyl‐thiamin‐diphosphate Intermediate of Transketolase with Hexacyanoferrate(III) or H₂O₂

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Nahrungsmittel und deren Hilfsstoffe

Cited by 2 publications

References 58 publications

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles

Production of Glycolate by Oxidation of the 1,2‐Dihydroxyethyl‐thiamin‐diphosphate Intermediate of Transketolase with Hexacyanoferrate(III) or H2O2

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Production of Glycolate by Oxidation of the 1,2‐Dihydroxyethyl‐thiamin‐diphosphate Intermediate of Transketolase with Hexacyanoferrate(III) or H₂O₂