NADPH supply and the contribution of NAD(P)+ transhydrogenase (NNT) to H2O2 balance in skeletal muscle mitochondria

Figueira, Tiago Rezende; Francisco, Annelise; Ronchi, Juliana A.; Santos, Guilherme R.R.M. dos; Santos, William dos; Treberg, Jason R.; Castilho, Roger F.

doi:10.1016/j.abb.2021.108934

Cited by 7 publications

(2 citation statements)

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“…Among the 5 genes, NNT (NAD(P)+ transhydrogenase) was the only one located in mitochondria. NNT supplies NADPH to remove endogenously generated H 2 O 2 [ 14 ] and was once suggested as a potential therapeutic target to help sustain the redox balance under condition of oxidative stress associated with neurodegenerative disease [ 15 ]. As normal mitochondrial function and redox balance are important for sperm [ 12 13 ], NNT may be a useful examination or therapeutic target related to damage from cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm

Fu,

Fang,

Guo

et al. 2024

World J Mens Health

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Purpose This study aimed to identify the altered pathways and genes associated with freezing damage in human sperm during cryopreservation by multiomics analysis. Materials and Methods Fifteen fresh human semen samples were collected for transcriptomic analysis, and another 5 fresh human semen samples were obtained for metabolomic analysis. For each semen sample, 1 mL was cryopreserved, and another 1 mL was left untreated for paired design. The results were then combined with previously published proteomic results to identify key genes/pathways. Results Cryopreservation significantly reduced sperm motility and mitochondrial structure. Transcriptomic analysis revealed altered mitochondrial function, including changes in tRNA-methyltransferase activity and adenosine tri-phosphate/adenosine di-phosphate transmembrane transporter activity. Metabolomic analysis showed that the citrate cycle in mitochondria was significantly altered. Combining transcriptomic, proteomic, and metabolomic analyses revealed 346 genes that were altered in at least two omics analyses. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that metabolic pathways were significantly altered and strongly associated with mitochondria. Five genes were altered in all three omics analyses: COL11A1 , COL18A1 , LPCAT3 , NME1 , and NNT . Conclusions Five genes were identified by multiomics analysis in human cryopreserved sperm. These genes might have specific functions in cryopreservation. Explorations of the functions of these genes will be helpful for sperm cryopreservation and sperm motility improvement or even for reproduction in the future.

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Section: Discussionmentioning

confidence: 99%

Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm

Fu,

Fang,

Guo

et al. 2024

World J Mens Health

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“…In the cytosol, NADP + is reduced to NADPH by the glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of the pentose phsophate pathway (PPP), amongst other reactions 13 . Whereas in the mitochondria, NADPH production is sustained by various reactions, including those reliant on nicotinamide nucleotide transhydrogenase (NNT) and isocitrate dehygronases (IDH2) enzyme activity 15 . As a result, NAD(P)(H) status has been shown to affect cell signaling and metabolism, energy homeostasis, mitochondrial biogenesis, oxidative stress responses and DNA maintenance and repair [16][17][18] .…”

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confidence: 99%

Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues

Azouaoui

Choinière

Khan

et al. 2023

Sci Rep

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Nicotinamide Adenine Dinucleotide (NAD+) plays an important role in energy metabolism and signaling pathways controlling crucial cellular functions. The increased interest in NAD+ metabolism and NAD+-boosting therapies has reinforced the necessity for accurate NAD+ quantification. To examine the published NAD(P)(H) measures across mammalian tissues, we performed a meta-analysis of the existing data. An Ovid MEDLINE database search identified articles with NAD(P)(H) quantification results obtained from mammalian tissues published between 1961 and 2021. We screened 4890 records and extracted quantitative data, as well as the quantification methods, pre-analytical conditions, and subject characteristics. The extracted physiological NAD(P)(H) concentrations in various tissues from mice, rats, and humans, revealed an important inter- and intra-method variability that extended to recent publications. This highlights the relatively poor potential for cross-experimental analyses for NAD(P)(H) quantitative data and the importance of standardization for NAD(P)(H) quantification methods and pre-analytical procedures for future preclinical and clinical studies.

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Nutraceuticals for targeting NAD+ to restore mitochondrial function

Garten

Lavery

2023

Molecular Nutrition and Mitochondria

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NADPH supply and the contribution of NAD(P)+ transhydrogenase (NNT) to H2O2 balance in skeletal muscle mitochondria

Cited by 7 publications

References 65 publications

Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm

Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm

Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues

Nutraceuticals for targeting NAD+ to restore mitochondrial function

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