NADPH oxidase of human dendritic cells: Role in <i>Candida albicans</i> killing and regulation by interferons, dectin‐1 and CD206

Donini, Marta; Zenaro, Elena; Tamassia, Nicola; Dusi, Stefano

doi:10.1002/eji.200636532

Cited by 53 publications

(47 citation statements)

References 56 publications

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“…MR is widely expressed on DC subsets and has been shown to play a role in the recognition of Candida. 153 A recent study revealed that MR promotes DCs to form "fungipod" upon contacting yeast-form Candida. "fungipod" is a dorsal pseudopodial protrusion and phagocytic structure of DCs, and the formation of this structure is propelled by the robust actin cytoskeleton growth at the DC-yeast contact site.…”

Section: Prrs Of Neutrophils and Monocytes/macrophagesmentioning

confidence: 99%

The role of pattern recognition receptors in the innate recognition ofCandida albicans

Zheng

Wang

et al. 2015

Virulence

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Section: Prrs Of Neutrophils and Monocytes/macrophagesmentioning

confidence: 99%

The role of pattern recognition receptors in the innate recognition ofCandida albicans

Zheng

Wang

et al. 2015

Virulence

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“…Macrophages and neutrophils use ROS, reactive nitrogen species (RNS), and chlorine species for host protection [6][7][8][9]63 , but the idea that ROS exert direct in vivo effects in the fungal killing of Candida and other species is still controversial. Balish et al 64 studied the deficient production of ROS and RNS in mice using gastrointestinal Candida inoculation.…”

Section: Acknowledgmentsmentioning

confidence: 99%

“…ROS contribute to the killing of C. albicans in both cultured cells and entire organisms [6][7][8][9] . Upon incubation with macrophages, C. albicans deoxyribonucleic acid (DNA) repair genes are transcriptionally induced, suggesting that DNA damage indeed occurs in the phagosome and that genotoxic hypersensitivity stress would be disadvantageous to the pathogen 10 .…”

mentioning

confidence: 99%

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

Abegg

Alabarse

Schüller

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Candida albicans, C. glabrata, C. tropicalis, and C. parapsilosis account for approximately 95% of identifiable Candida infections. Other species, including C. krusei, C. lusitaniae, and C. guilliermondii, account for less than 5% of cases of invasive candidiasis. The most common causative agent is still C. albicans, but its incidence is declining and the frequencies of other species are increasing. Recently, Furlaneto et al. 1 noted that non-albicans Candida was the predominant species in different clinical specimens, with the exception of urine samples, in a Brazilian tertiary-care hospital. Invasive candidiasis has a mortality rate that approaches 40% 2,3 . Although most people are colonized by Candida sp., the majority never develop invasive candidiasis. Alterations in host immunity, physiological features, or normal microflora, rather than the acquisition of novel or hypervirulent factors by Candida, are suggested to degenerate the commensal-host interaction and lead to an opportunistic infection 4 .During the course of a systemic infection, Candida cells are engulfed by host phagocytes, where they are exposed to reactive oxygen species (ROS) 5 . ROS contribute to the killing of C. albicans in both cultured cells and entire organisms [6][7][8][9] . Upon incubation with macrophages, C. albicans deoxyribonucleic acid (DNA) repair genes are transcriptionally induced, suggesting that DNA damage indeed occurs in the phagosome and that genotoxic hypersensitivity stress would be disadvantageous to the pathogen 10 . Recently, it was demonstrated that a large proportion of C. albicans cell surface antigens related to acute candidemia are involved in oxidative stress 4 . In C. albicans, hyphal cells ABSTRACT Introduction: The capacity to overcome the oxidative stress imposed by phagocytes seems to be critical for Candida species to cause invasive candidiasis. Methods: To better characterize the oxidative stress response (OSR) of 8 clinically relevant Candida sp., glutathione, a vital component of the intracellular redox balance, was measured using the 5,5'-dithiobis-(2-nitrobenzoic acid (DTNB)-glutathione disulfide (GSSG) reductase reconversion method; the total antioxidant capacity (TAC) was measured using a modified method based on the decolorization of the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic) acid radical cation (ABTS* + ). Both methods were used with cellular Candida sp. extracts treated or not with hydrogen peroxide (0.5 mM). Results: Oxidative stress induced by hydrogen peroxide clearly reduced intracellular glutathione levels. This depletion was stronger in Candida albicans and the levels of glutathione in untreated cells were also higher in this species. The TAC demonstrated intra-specific variation. Conclusions: Glutathione levels did not correlate with the measured TAC values, despite this being the most important non-enzymatic intracellular antioxidant molecule. The results indicate that the isolated measurement of TAC does not give a clear picture of the ability of a given Candida sp. to resp...

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“…Dectin-1 mediated uptake of C. albicans and P. carinii leads to killing of the ingested fungus in part through the production of ROS as well as production of inflammatory mediators such as TNF-α and the chemokine CXCL2 [43]. A recent study, however, indicated that C. albicans does not use Dectin-1 to invade human monocytederived DC and does not induce NADPH oxidase activity [44]. As previously mentioned, TLR2 and Dectin-1 cooperate in the macrophage production of various immune modulators including TNF-α, MIP-2, IL-12p40 and ROS [31].…”

Section: Dectin-1 and The Immune Responsementioning

confidence: 99%

The Pattern Recognition Receptor Dectin-1: From Fungi to Mycobacteria

Schorey¹,

Lawrence

2008

CDT

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The ability of the innate immune system to quickly recognize and respond to an invading pathogen is essential for controlling the infection. For this purpose, cells of the immune system express receptors which recognize evolutionarily conserved structures expressed by various pathogens but absent from host cells. In this review we focus on the non-classical C-type lectin receptors including Dectin-1 whose role has been extensively characterized in the recognition and response to fungal pathogens. Dectin-1 is a type II transmembrane protein which binds β-1,3 and β-1,6 glucans. It is expressed on most cells of the innate immune system and has been implicated in phagocytosis as well as killing of fungi by macrophages, neutrophils and dendritic cells. The Dectin-1 cytoplasmic tail contains an immunoreceptor tyrosine based activation motif (ITAM) that signals in part through the spleen tyrosine kinase and in collaboration with Toll-like receptors. Although the main research focus has been on Dectin-1's role as a fungal and yeast pathogen recognition receptor, more recent studies suggest that Dectin-1 may have a broader function in pathogen recognition including a role in directing a macrophage response to mycobacterial infections.

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NADPH oxidase of human dendritic cells: Role in Candida albicans killing and regulation by interferons, dectin‐1 and CD206

Cited by 53 publications

References 56 publications

The role of pattern recognition receptors in the innate recognition ofCandida albicans

The role of pattern recognition receptors in the innate recognition ofCandida albicans

Glutathione levels in and total antioxidant capacity of Candida sp. cells exposed to oxidative stress caused by hydrogen peroxide

The Pattern Recognition Receptor Dectin-1: From Fungi to Mycobacteria

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