NADPH oxidase contributes to oxidative damage and mitochondrial impairment induced by acute ethanol treatment in rat hippocampal neurons

Pérez, Marı́a José; Loyola, Rocío; Canelo, Francisco; Aránguiz, Alejandra; Tapia-Monsalves, Carola; Osorio‐Fuentealba, Cesár; Quintanilla, Rodrigo A.

doi:10.1016/j.neuropharm.2020.108100

Cited by 11 publications

(5 citation statements)

References 40 publications

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Section: Methodsmentioning

confidence: 99%

“…Superoxide levels were evaluated with MitoSox™ (Molecular Probes, Thermo Fisher Scientific) and the mitochondrial dye MitoTracker Deep Red FM (Pérez et al, 2020 ). Transfected cultured neurons were incubated with 0.5 μ M MitoSox and 0.3 μ M MitoTracker Deep Red in KRH – glucose buffer at 37°C for 30 min (Quintanilla et al, 2013 ; Pérez et al, 2020 ). For superoxide determinations with cell imaging, fluorescence images were acquired using the same exposure time and gain to minimize dye photo-bleaching (Quintanilla et al, 2013 ; Pérez et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

“…Transfected cultured neurons were incubated with 0.5 μ M MitoSox and 0.3 μ M MitoTracker Deep Red in KRH – glucose buffer at 37°C for 30 min (Quintanilla et al, 2013 ; Pérez et al, 2020 ). For superoxide determinations with cell imaging, fluorescence images were acquired using the same exposure time and gain to minimize dye photo-bleaching (Quintanilla et al, 2013 ; Pérez et al, 2020 ). MitoSox fluorescence ( F ) was determined in each cell, and background ( F 0) of each image was subtracted before normalization per cell area.…”

Section: Methodsmentioning

confidence: 99%

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Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells

Quintanilla

Tapia-Monsalves

Vergara

et al. 2020

Front. Cell. Neurosci.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells

Quintanilla

Tapia-Monsalves

Vergara

et al. 2020

Front. Cell. Neurosci.

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“…One study found that acute ethanol treatment can induce neurodegeneration in cultured hippocampal neurons, leading to mitochondrial dysfunction, calcium processing defects, and synaptic damage in hippocampal neurons. This means that mitochondrial-related genes (such as tomm7) also play a role in this process (Pérez et al, 2020). Another study found that during the withdrawal period after alcohol exposure, the expression of miRNAs (microRNAs) and mRNAs (messenger RNAs) in the rat hippocampus area changed significantly, including some genes related to muscle development.…”

Section: Discussionmentioning

confidence: 99%

Salidroside ameliorates memory impairment following long-term ethanol intake in rats by modulating the altered intestinal microbiota content and hippocampal gene expression

Jiao

Zhao

et al. 2023

Front. Microbiol.

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BackgroundSalidroside (Sal), the main component of a famous herb Rhodiola rosea L, enhances memory performance and reduces fatigue. Therefore, this study assessed the effect of Sal on memory impairment induced by a long-term intake of ethanol (EtOH) in rats and investigated its relevant mechanisms using gut microbiota metagenomic analysis and hippocampal transcriptomic analysis.MethodsEighteen male SD rats were divided into the normal control group (CON group), EtOH model group (Model group), and Sal treatment group (Sal group). The rats in the Model and Sal groups intragastrically (i.g.) received 2 g/kg EtOH for 30 consecutive days, whereas the CON group was given an equal volume of distilled water. Meanwhile, the rats in the Sal group were administered i.g. 30 mg/kg Sal 60 min after EtOH intake. All rats were tested in the eight-arm maze for their memory function every 3 days. On the 30th day, metagenomic analyses of gut microbiota and transcriptomic analyses of the hippocampus were performed.ResultsCompared with the Model group, Sal treatment reduced the total time to complete the eight-arm maze task, decreased the number of arm entries, and abated the working memory error that was significant from the 9th day. Additionally, Sal intervention improved the gut microbiota composition, such as the increased abundance of Actinobacteria and Bifidobacterium, which was related to the metabolism of amino acids and terpenoid carbohydrate, endocrine function, and signal transduction by neurotransmitters. In the hippocampus, the EtOH intake differentially expressed 68 genes (54 genes increased, whereas 14 genes decreased), compared with the CON group, whereas Sal intervention affected these changes: 15 genes increased whereas 11 genes decreased. And, enrichment analyses revealed these genes were related to the structural components of the ribosome, mRNA splicing process, protein translation, mitochondria function, and immunological reaction. Finally, a correlation analysis found the memory impairment was positively correlated with the abnormal upregulation of Tomm7 but negatively correlated with decreased abundance of gut Alistipes_indistinctus, Lactobacillus_taiwanensis, Lactobacillus_paragasseri, and Lactobacillus johnsonii.ConclusionSal improved memory impairment caused by long-term EtOH intake in rats, which may be related to its regulation of gut dysbiosis and hippocampal dysfunction.

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“…The role of altered expression of NO synthases does not seem to be consistent (Yazir et al, 2012). Chronic alcohol consumption reliably produces oxidative stress and inflammation in the liver and the brain (Osna et al, 2017; Perez et al, 2020) and thus might also contribute to ED. In line with this, NADPH oxidase‐derived reactive oxygen species are increased in the CC after chronic alcohol consumption in the rat (Leite et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice

Olivencia

Biedma-Elduayen

Giménez-Gómez

et al. 2023

British J Pharmacology

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Background and purposeAlcohol abuse has been associated with erectile dysfunction (ED), but the implicated molecular mechanisms are unresolved. This study analyses the role of alterations in soluble guanylyl cyclase (sGC) in ED.Experimental approachED was analysed in adult male C57BL/6J mice subjected to the Chronic Intermittent Ethanol (CIE) paradigm. Erectile function was assessed in anaesthetised mice in vivo by evaluating intracavernosal pressure (ICP) and in vitro in isolated mice corpora cavernosa (CC) mounted in a myograph. Protein expression and reactive oxygen species were analysed by western blot and dihydroethidium staining, respectively.Key resultsIn CIE mice, we observed a significant decrease in the relaxant response of the CC to stimulation of NO release from nitrergic nerves by electrical field stimulation, to NO release from endothelial cells by acetylcholine, to the PDE5 inhibitor sildenafil, and to the sGC stimulator riociguat. Conversely, the response to the sGC activator cinaciguat, whose action is independent of the oxidation state of sGC, was significantly enhanced in these CC. The responses to adenylyl cyclase stimulation with forskolin were unchanged. We found an increase in reactive oxygen species in the CC from CIE mice as well as an increase in CYP2E1 and NOX2 protein expression. In vivo pre‐treatment with tempol prevented alcohol‐induced erectile dysfunction.Conclusions and implicationsOur results demonstrate that alcoholic mice show ED in vitro and in vivo due to an alteration in the redox state of sGC and suggest that sGC activators may be effective in ED associated with alcoholism.

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NADPH oxidase contributes to oxidative damage and mitochondrial impairment induced by acute ethanol treatment in rat hippocampal neurons

Cited by 11 publications

References 40 publications

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells

Truncated Tau Induces Mitochondrial Transport Failure Through the Impairment of TRAK2 Protein and Bioenergetics Decline in Neuronal Cells

Salidroside ameliorates memory impairment following long-term ethanol intake in rats by modulating the altered intestinal microbiota content and hippocampal gene expression

Oxidized soluble guanylyl cyclase causes erectile dysfunction in alcoholic mice

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