NADPH-diaphorase activity and nitric oxide synthase isoforms in the trophoblast of Calomys callosus

Moraes, Neci; Zago, Douglas; Gagioti, S; Hoshida, Mara Sandra; Bevilacqua, Estela Maris Andrade Forell

doi:10.1017/s002187820100749x

Cited by 7 publications

(3 citation statements)

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“…Likewise the prominent role of maternal uNK cells in placentation is a feature that sigmodonts share with other cricetid and murid rodents [13,46]. The most striking finding in the present material was the relative abundance of trophoblast giant cells first described in Calomys [19] and here extended to a further five genera. In murid rodents giant cells produce a wide range of hormones and cytokines such as proliferin [59] and the significance of the expanded giant cell population in sigmodonts cries for closer attention.…”

Section: Discussionsupporting

confidence: 77%

Placentation in Sigmodontinae: a rodent taxon native to South America

Favaron

Carter

Ambrósio

et al. 2011

Reprod Biol Endocrinol

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BackgroundSigmodontinae, known as "New World rats and mice," is a large subfamily of Cricetidae for which we herein provide the first comprehensive investigation of the placenta.MethodsPlacentas of various gestational ages ranging from early pregnancy to near term were obtained for five genera, i.e. Necromys, Euryoryzomys, Cerradomys, Hylaeamys, and Oligoryzomys. They were investigated by means of histology, immunohistochemistry, a proliferation marker, DBA-lectin staining and transmission electron microscopy.ResultsThe chorioallantoic placenta was organized in a labyrinthine zone, spongy zone and decidua and an inverted yolk sac persisted until term. The chorioallantoic placenta was hemotrichorial. The interhemal barrier comprised fetal capillary endothelium and three layers of trophoblast, an outermost, cellular layer and two syncytial ones, with interspersed trophoblast giant cells (TGC). In addition, accumulations of TGC occurred below Reichert's membrane. The junctional zone contained syncytial trophoblast, proliferative cellular trophoblast, glycogen cells and TGC that were situated near to the maternal blood channels. In three of the genera, TGC were also accumulated in distinct areas at the placental periphery. PAS-positive glycogen cells derived from the junctional zone invaded the decidua. Abundant maternal uNK cells with positive response to PAS, vimentin and DBA-lectin were found in the decidua. The visceral yolk sac was completely inverted and villous.ConclusionThe general aspect of the fetal membranes in Sigmodontinae resembled that found in other cricetid rodents. Compared to murid rodents there were larger numbers of giant cells and in some genera these were seen to congregate at the periphery of the placental disk. Glycogen cells were found to invade the decidua but we did not identify trophoblast in the walls of the deeper decidual arteries. In contrast these vessels were surrounded by large numbers of uNK cells. This survey of wild-trapped specimens from five genera is a useful starting point for the study of placentation in an important subfamily of South American rodents. We note, however, that some of these rodents can be captive bred and recommend that future studies focus on the study of time dated pregnancies.

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Section: Discussionsupporting

confidence: 77%

Placentation in Sigmodontinae: a rodent taxon native to South America

Favaron

Carter

Ambrósio

et al. 2011

Reprod Biol Endocrinol

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“…NOS have been shown to have NADPH‐diaphorase activity, as evidenced by colocalization and coprecipitation of NADPH‐diaphorase and NOS activity (Snyder, 1992). In fact, NADPH‐diaphorase histochemistry has been widely used as an indirect way to determine in situ NOS activity (Kugler et al, 1994; Roufail et al, 1995; Moraes et al, 2001). Lung sections, 40‐μm thickness, were cut using a cryostat (2800 Frigocut E, Reichert‐Jung Vienna, Austria).…”

Section: Methodsmentioning

confidence: 99%

Lung eNOS and iNOS are Reoxygenation Time‐Dependent Upregulated After Acute Hypoxia

Rus

Peinado

Castro

et al. 2010

The Anatomical Record

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Nitric oxide plays a critical role in many physiological and physiopathological processes in the lung. Changes in the NO/NOS (Nitric Oxide/Nitric Oxide Synthase) system after hypoxia situations remain controversial in this organ, so that the aim of this work is to perform a complete study of this system in the hypoxic lung after different reoxygenation times ranging from 0 h to 5 days posthypoxia. This is a novel follow-up study carried out in Wistar rats submitted for 30 min to acute hypobaric hypoxia. We measured endothelial and inducible NOS (eNOS, iNOS) mRNA and protein expression, location, and in situ NOS activity as well as nitrated protein expression and location. In addition, NO levels were indirectly quantified (NOx) as well as the apoptosis level. Results showed an increase in eNOS mRNA, protein, activity as well as eNOS positive immunostaining at 0 h posthypoxia, coinciding with raised NOx levels. Contrary, iNOS, nitrated protein expression and apoptosis level augmented during the final reoxygenation times. The lung NO/NOS system provokes two responses to the hypoxia/reoxygenation processes: (i) eNOS is responsible of the immediate response, producing NO, which causes vasodilation and bronchodilation, and (ii) iNOS is related to the second late response, which seems to be involved in some of the deleterious consequences that hypoxia induces in the lung.

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“…The NADPH‐d activity was related to the oestrus phase or days of pregnancy. Moraes et al. (2001) localized NADPH‐d and iNOS activity in the Calomys callosus subpopulation of the placental trophoblast cells in various stages of pregnancy.…”

Section: Discussionmentioning

confidence: 98%

Expression Patterns of Endothelial and Inducible Nitric Oxide Isoforms in the Porcine Umbilical Cord

Chruściel

Andronowska

Postek

2009

Reprod Domestic Animals

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Stable fetal-placental blood pressure and flow are extremely important in fetal growth and development. Uncontrolled and long-standing increased or decreased vascular blood pressure in the umbilical cord (UC) affects hyperaemia or ischaemia and consequently causes fetal death. Nitric oxide (NO) is one of the most active factors controlling blood flow through relaxation of the vascular smooth muscle. In this study, we investigated endothelial (eNOS) and inducible (iNOS) nitric oxide synthase expression and NADPH-diaphorase activity (NADPH-d) in the porcine UC at various stages of pregnancy. The UCs were collected from pigs on days 40, 60, 75 and 90 of pregnancy and postpartum. Western blot analysis as well as immunohistochemical staining revealed protein presence for eNOS and iNOS in the UC of the pig. The eNOS expression was maintained at a significantly higher level in all analysed days of pregnancy when compared with postnatal stage. Additionally, a significant protein increase for eNOS was observed in a periplacental part of UC on day 90. There were no obvious differences in iNOS protein level in UC samples derived from different stages of pregnancy. NADPH-diaphorase histochemical activity was correlated with NOS immunoreactivity during all analysed days of pregnancy. These results suggest that NOS isoforms are responsible for regulation of blood circulation in UC and immune responses.

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NADPH-diaphorase activity and nitric oxide synthase isoforms in the trophoblast of Calomys callosus

Cited by 7 publications

References 0 publications

Placentation in Sigmodontinae: a rodent taxon native to South America

Placentation in Sigmodontinae: a rodent taxon native to South America

Lung eNOS and iNOS are Reoxygenation Time‐Dependent Upregulated After Acute Hypoxia

Expression Patterns of Endothelial and Inducible Nitric Oxide Isoforms in the Porcine Umbilical Cord

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