NADPH Dependent superoxide generation in the ovary and uterus of mice during estrous cycle and early pregnancy

Jain, Sudhir; Saxena, Deeksha; Kumar, Pratap; Laloraya, Malini

doi:10.1016/s0024-3205(00)00417-3

Cited by 20 publications

(9 citation statements)

References 24 publications

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“…Hydrogen peroxide is a well-documented agent for the generation of intracellular ROS [Barbieri et al 2003] and as shown above in cultured endometrial cells, our result suggests that ADM may modify the level of ROS in the female reproductive tract. Thus ADM may protect the sperm from oxidative damage caused by ROS generated by leukocytes and dead spermatozoa in the ejaculate [Hammadeh et al 2009] or by the uterus [Jain et al 2000]. Post-coital uterine flushing was also found to decrease ROS, in agreement with our previous finding of a protective role of accessory gland secretion against oxidative stress in the sperm [Chen et al 2002].…”

Section: Discussionsupporting

confidence: 89%

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

Liao

Kong²,

Tang³

et al. 2012

Systems Biology in Reproductive Medicine

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Adrenomedullin (ADM) is found in male accessory sex glands and is part of the seminal secretion. It plays an important role in protecting the sperm in the female reproductive tract. In this study, we investigated the roles of ADM in inflammation and oxidative stress in the endometrium and in leukocyte and macrophage infiltration in the endometrial stroma. The expression of the ADM gene in the ventral prostate, coagulating gland, and seminal vesicle was determined by real time PCR. The peptide levels in the tissue and secretion were measured using an EIA Kit. The highest ADM mRNA and peptide levels were found in the ventral prostate. Most of the ADM in the seminal vesicle was stored in the tissue while little was secreted. The expression of the IL-1β gene and the secretion of TNFα and IL-6 in uterine tissue decreased significantly after treatment with ADM for 4 hours. Using an immunostaining method, the levels of leukocyte and macrophage infiltration were found to be lower at 24 hours post coitus than 1.5 hours post coitus. The infusion of ADM receptor antagonist reduced the infiltration of leukocyte and macrophages in the endometrial stroma at 24 hours post coitus. As to the anti-oxidative effect of ADM in the female tract, the reactive oxygen species (ROS) level in isolated endometrial epithelial cells was significantly decreased after treatment with ADM or seminal fluid. Our findings demonstrated that ADM in the seminal secretion may modify the inflammatory responses, play an anti-oxidative role, and increase leukocyte and macrophage infiltration in the uterus.

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Section: Discussionsupporting

confidence: 89%

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

Liao

Kong²,

Tang³

et al. 2012

Systems Biology in Reproductive Medicine

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“…As for the nature of the generator, in the case of . O 2 -, it appears to be represented by NADPH at proestrus as well as at pre-implantation phase [60].…”

Section: Oxidative Homeostasis In Pregnancy Early Pregnancymentioning

confidence: 94%

The Role and Modulation of the Oxidative Balance in Pregnancy

Biondi¹,

Pavan²,

Lunghi³

et al. 2005

CPD

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Oxidative processes exert a fundamental regulatory function during pregnancy. It depends on the influence of oxygen, nitric oxide, reactive oxygen species and reactive nitrogen species metabolic pathways upon the vascular changes in the maternal organism, as well as on the regulation of uterine and cervical tone throughout gestation and delivery. These functions are strictly linked with the mediators of the inflammatory pathway. At the beginning of pregnancy, when a certain grade of inflammatory change is necessary to the trophoblast invasion of maternal tissue, the activation of the process by nitric oxide and reactive nitrogen species is welcome. Indeed, these products modulate the metalloproteinases, which are responsible for the remodelling of uterine extracellular matrix. At this stage estrogens are involved as well in the regulation of the delicate balance of pro-oxidant and anti-oxidant effects. Furthermore, reactive oxygen and nitrogen species appear to play an important role both in normal and pathologic embryogenesis. During advanced pregnancy, a derangement of the oxidative balance can lead to the improper activation of inflammatory changes, thus triggering premature labour as well as other complications, such as foetal growth restriction and preeclampsia. Although a number of pro- and anti-oxidant agents are available to influence the above-mentioned processes, there is no way to adequately measure the oxidative needs in single cases, in order to modulate the oxidative balance in clinical practice. Pharmacological research should be addressed to the development of new drugs, as well as to selective methods of delivery to the gestational tissues.

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“…ROS levels in the corpus luteum actually increase during the regression phase [39-44]. The NADPH-dependent generation of superoxide in the mouse ovary increases during the early pre-ovulatory phase in cycling females and during the luteal phase in pregnant animals [45]. Ovarian as well as uterine NADPH-dependent superoxide production appears to be LH-inducible.…”

Section: Physiological Relevance Of Ros/rnos and Antioxide/redox Enzymentioning

confidence: 99%

Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system

Fujii

Iuchi

Okada

2005

Reprod Biol Endocrinol

230

120

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Controlled oxidation, such as disulfide bond formation in sperm nuclei and during ovulation, plays a fundamental role in mammalian reproduction. Excess oxidation, however, causes oxidative stress, resulting in the dysfunction of the reproductive process. Antioxidation reactions that reduce the levels of reactive oxygen species are of prime importance in reproductive systems in maintaining the quality of gametes and support reproduction. While anti-oxidative enzymes, such as superoxide dismutase and peroxidase, play a central role in eliminating oxidative stress, reduction-oxidation (redox) systems, comprised of mainly glutathione and thioredoxin, function to reduce the levels of oxidized molecules. Aldo-keto reductase, using NADPH as an electron donor, detoxifies carbonyl compounds resulting from the oxidation of lipids and proteins. Thus, many antioxidative and redox enzyme genes are expressed and aggressively protect gametes and embryos in reproductive systems.

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NADPH Dependent superoxide generation in the ovary and uterus of mice during estrous cycle and early pregnancy

Cited by 20 publications

References 24 publications

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

Possible functions of adrenomedullin from the seminal fluid in the female reproductive tract of the rat

The Role and Modulation of the Oxidative Balance in Pregnancy

Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system

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