NADP-malic enzyme from plants

Ge, Edwards; Andreo, CS

doi:10.1016/0031-9422(92)80322-6

Cited by 131 publications

(19 citation statements)

References 80 publications

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“…A relationship between the non-photosynthetic isoform of NADP-ME and plant defence response was suggested in our previous work and also by other authors [4–10]. NADP-ME catalyzes the oxidative decarboxylation of l -malate using NADP + as a coenzyme in the presence of divalent metal ions (Mg 2+ or Mn 2+ ions) to produce pyruvate, NADPH and CO 2 [11,12]. The presence of a cofactor and the coenzyme is required for the catalysis.…”

Section: Introductionmentioning

confidence: 77%

“…Besides this, NADP-ME in plants is also localized in chloroplasts, well known is its photosynthetic function in some C 4 plants ( e.g., maize). Supporting functions such as providing NADPH for assimilatory process ( e.g., lipid biosynthesis), maintaining intracellular pH [11] and above-mentioned implication in plant defence to stress are proposed for non-photosynthetic C 3 NADP-ME isoform localized in chloroplasts and/or in cytosol [12,15]. NADPH production is also necessary for biosynthesis of specific defence compounds ( e.g., phytoalexins, lignins, osmolytically active compounds) important under stress [12] or act as cofactor for antioxidative enzymes [3].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

Doubnerová

Müller

Čeřovská

et al. 2009

IJMS

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The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVYO. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVYNTN strain than in the case of strain PVYO. The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

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Section: Introductionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

Doubnerová

Müller

Čeřovská

et al. 2009

IJMS

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“…It is generally accepted that alkaline stress is characterized by high pH value, and always causes much stronger inhibition of plant growth than salt stress [41]. Previous studies showed that NADP-ME could help to maintain the cytosolic pH homeostasis[42], and citric acid was an indicator of plant responses to pH challenge [43]. Therefore, we investigated the NADP-ME activity and citrate acid content of WT and transgenic plants, in an attempt to understand the physiological mechanisms responsible for the increased alkaline tolerance of GsTIFY10a transgenic alfalfa.…”

Section: Resultsmentioning

confidence: 99%

The Positive Regulatory Roles of the TIFY10 Proteins in Plant Responses to Alkaline Stress

Zhu

Liu

et al. 2014

PLoS ONE

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The TIFY family is a novel plant-specific protein family, and is characterized by a conserved TIFY motif (TIFF/YXG). Our previous studies indicated the potential roles of TIFY10/11 proteins in plant responses to alkaline stress. In the current study, we focused on the regulatory roles and possible physiological and molecular basis of the TIFY10 proteins in plant responses to alkaline stress. We demonstrated the positive function of TIFY10s in alkaline responses by using the AtTIFY10a and AtTIFY10b knockout Arabidopsis, as evidenced by the relatively lower germination rates of attify10a and attify10b mutant seeds under alkaline stress. We also revealed that ectopic expression of GsTIFY10a in Medicago sativa promoted plant growth, and increased the NADP-ME activity, citric acid content and free proline content but decreased the MDA content of transgenic plants under alkaline stress. Furthermore, expression levels of the stress responsive genes including NADP-ME, CS, H+-ppase and P5CS were also up-regulated in GsTIFY10a transgenic plants under alkaline stress. Interestingly, GsTIFY10a overexpression increased the jasmonate content of the transgenic alfalfa. In addition, we showed that neither GsTIFY10a nor GsTIFY10e exhibited transcriptional activity in yeast cells. However, through Y2H and BiFc assays, we demonstrated that GsTIFY10a, not GsTIFY10e, could form homodimers in yeast cells and in living plant cells. As expected, we also demonstrated that GsTIFY10a and GsTIFY10e could heterodimerize with each other in both yeast and plant cells. Taken together, our results provided direct evidence supporting the positive regulatory roles of the TIFY10 proteins in plant responses to alkaline stress.

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“…When analyzing the candidate genes identified by each method separately, they were enriched for different functions. Tajima's D outliers were enriched for transcription factors; X T X outliers were enriched for genes involved in protein degradation; LFMM candidate genes were enriched for the enzyme malate dehydrogenase (involved in pyruvate metabolism and carbon fixation; Edwards & Andreo, 1992) and proteasome activator subunit 4 (involved in degradation of histones during DNA damage response, Book et al., 2010); and BAYENV2 candidate genes were enriched for genes involved in disease resistance, protein degradation, ethylene signaling, RNA splicing, protein synthesis, and stress response, among others (Table S6). The only category shared by all candidate gene sets is the enrichment for targets of the transcriptional regulators ARK1 and ARK2 involved in wood formation.…”

Section: Discussionmentioning

confidence: 99%

Population genomics of the eastern cottonwood (Populus deltoides)

Fahrenkrog

Neves

Resende

et al. 2017

Ecology and Evolution

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Despite its economic importance as a bioenergy crop and key role in riparian ecosystems, little is known about genetic diversity and adaptation of the eastern cottonwood (Populus deltoides). Here, we report the first population genomics study for this species, conducted on a sample of 425 unrelated individuals collected in 13 states of the southeastern United States. The trees were genotyped by targeted resequencing of 18,153 genes and 23,835 intergenic regions, followed by the identification of single nucleotide polymorphisms (SNPs). This natural P. deltoides population showed low levels of subpopulation differentiation (F ST = 0.022–0.106), high genetic diversity (θW = 0.00100, π = 0.00170), a large effective population size (N e ≈ 32,900), and low to moderate levels of linkage disequilibrium. Additionally, genomewide scans for selection (Tajima's D), subpopulation differentiation (XTX), and environmental association analyses with eleven climate variables carried out with two different methods (LFMM and BAYENV2) identified genes putatively involved in local adaptation. Interestingly, many of these genes were also identified as adaptation candidates in another poplar species, Populus trichocarpa, indicating possible convergent evolution. This study constitutes the first assessment of genetic diversity and local adaptation in P. deltoides throughout the southern part of its range, information we expect to be of use to guide management and breeding strategies for this species in future, especially in the face of climate change.

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NADP-malic enzyme from plants

Cited by 131 publications

References 80 publications

Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

The Positive Regulatory Roles of the TIFY10 Proteins in Plant Responses to Alkaline Stress

Population genomics of the eastern cottonwood (Populus deltoides)

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