NADH-Monodehydroascorbate Oxidoreductase Is One of the Redox Enzymes in Spinach Leaf Plasma Membranes1

Bérczi, Alajos; Møller, Ian Max

doi:10.1104/pp.116.3.1029

Cited by 76 publications

(37 citation statements)

References 53 publications

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“…In plants, MDHAR activity has been detected in the chloroplast, mitochondria, peroxisome, cytosol, and associated with the plasma membrane and cell wall (Dalton et al 1993;Leonardis et al 1995;Bérczi and Møller 1998;Mittova et al 2000). In this study, changes in MDHAR activity were varies depending on the development stage and the intensity and duration of water stress, with a marked increase in fruits at the mature green stage of plants stressed for 6 and 10 days (Fig.…”

Section: Discussionsupporting

confidence: 50%

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Murshed

Lopez-Lauri²,

Sallanon

2013

Physiol Mol Biol Plants

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The effects of different levels of water stress on oxidative parameters (H 2 O 2 and MDA), the total pool sizes of ascorbate, the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the activities and relative transcript levels of the enzymes of ascorbate-glutathione cycle ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) were studied in the fruit of tomato (Solanum lycopersicum L. cv. Micro-Tom). Plants were subjected to three levels of water stress (S50, S25 and S0) and fruits at different development stages were harvested after 3, 6 and 10 days of stress. Changes in H 2 O 2 and MDA contents indicated that water stress induced oxidative stress in fruits. The concentrations of ascorbate (AsA) and dehydroascorbate (DHA) generally modified with water stress treatments. Moreover, changes in SOD and CAT activities and DHAR, MDHAR, APX and GR activities and relative transcript levels were dependent on the fruit development stage and the intensity and the duration of water stress. These results suggest that the response of antioxidant systems of tomato fruits to oxidative stress induced by water stress treatments was different depending on the fruit development stage.

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Section: Discussionsupporting

confidence: 50%

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Murshed

Lopez-Lauri²,

Sallanon

2013

Physiol Mol Biol Plants

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“…Assuming that the flavin content of spinach leaf PM is similar to that of PM from other sources (0·2-0·5 nmol mg -1 protein, Table 2) then MDAR contributes only about 10% of the total flavin and about 20% of the FAD in the PM. Any inactivation of the enzyme during purification would lower this value, but the MDAR activity appears to be quite stable under a variety of experimental conditions (Bérczi & Møller 1998a). Thus, MDAR is not the only flavoprotein in the PM.…”

Section: Monodehydroascorbate Reductasementioning

confidence: 99%

“…The PM from spinach leaves completely stripped of MDAR contained about 15% of the original NFR activity (Bérczi & Møller 1998a) and had relatively increased activ-…”

Section: Monodehydroascorbate Reductasementioning

confidence: 99%

“…enzyme, a detailed fractionation procedure was performed in order to reveal the in vivo localization of the enzyme (Bérczi & Møller 1998a). The right-side-out PM vesicles obtained by two-phase partitioning were subjected to consecutive salt-washing and detergent (Brij-58 and CHAPS) treatments, and the fractions were analysed for both NFR and MDAR activities.…”

Section: Monodehydroascorbate Reductasementioning

confidence: 99%

“…An NFR from spinach leaf PM was purified by , but only 3 years later it was identified as MDAR based on a high degree of sequence identity, substrate specificity, inhibitor sensitivity, kinetic constants, and immunological cross-reactivity (Bérczi & Møller 1998a). The purified enzyme from spinach leaves has an apparent molecular mass of 45 kDa estimated by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE), pI values of 5·3-5·6 (three spots, probably isoforms, were detected) determined by isoelectricfocusing (IEF), prefers NADH to NADPH, contains one FAD per 45 kDa polypeptide , and is specific for the b-hydrogen on NADH .…”

Section: Monodehydroascorbate Reductasementioning

confidence: 99%

See 2 more Smart Citations

Redox enzymes in the plant plasma membrane and their possible roles

Bérczi

Møller

2000

Plant Cell & Environment

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PlantL-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing

Davey

Montagu

Inzé

et al. 2000

J. Sci. Food Agric.

1,065

173

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Humans are unable to synthesise L‐ascorbic acid (L‐AA, ascorbate, vitamin C), and are thus entirely dependent upon dietary sources to meet needs. In both plant and animal metabolism, the biological functions of L‐ascorbic acid are centred around the antioxidant properties of this molecule. Considerable evidence has been accruing in the last two decades of the importance of L‐AA in protecting not only the plant from oxidative stress, but also mammals from various chronic diseases that have their origins in oxidative stress. Evidence suggests that the plasma levels of L‐AA in large sections of the population are sub‐optimal for the health protective effects of this vitamin. Until quite recently, little focus has been given to improving the L‐AA content of plant foods, either in terms of the amounts present in commercial crop varieties, or in minimising losses prior to ingestion. Further, while L‐AA biosynthesis in animals was elucidated in the 1960s,1 it is only very recently that a distinct biosynthetic route for plants has been proposed.2 The characterisation of this new pathway will undoubtedly provide the necessary focus and impetus to enable fundamental questions on plant L‐AA metabolism to be resolved. This review focuses on the role of L‐AA in metabolism and the latest studies regarding its biosynthesis, tissue compartmentalisation, turnover and catabolism. These inter‐relationships are considered in relation to the potential to improve the L‐AA content of crops. Methodology for the reliable analysis of L‐AA in plant foods is briefly reviewed. The concentrations found in common food sources and the effects of processing, or storage prior to consumption are discussed. Finally the factors that determine the bioavailability of L‐AA and how it may be improved are considered, as well as the most important future research needs. © 2000 Society of Chemical Industry

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NADH-Monodehydroascorbate Oxidoreductase Is One of the Redox Enzymes in Spinach Leaf Plasma Membranes1

Cited by 76 publications

References 53 publications

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum lycopersicon L, cv. Micro-tom)

Redox enzymes in the plant plasma membrane and their possible roles

PlantL-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing

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