NADH-dependent glutamate synthase participated in ammonium assimilation in<i>Arabidopsis</i>root

Kojima, Shinichi; Konishi, Noriyuki; Beier, Marcel Pascal; Ishiyama, Keiki; Maru, Ikumi; Hayakawa, Toshihiko; Yamaya, Tomoyuki

doi:10.4161/psb.29402

Cited by 19 publications

(13 citation statements)

References 25 publications

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“…Free amino acids and NH 4 + contents in spikelet and flag leaf were measured according to our previous experiment with slightly modification (Kojima et al 2014 ). Spikelets and flag leaves were harvested in 2.0 mL safe lock tube (Eppendorf Co., Ltd., Tokyo, Japan) with zirconia beads and stored at −80 °C until free amino acid extraction.…”

Section: Methodsmentioning

confidence: 99%

A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains

Yabuki

Ohashi

Imagawa

et al. 2017

Rice

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BackgroundAsparagine is one of the most dominant organic nitrogen compounds in phloem and xylem sap in a wide range of plant species. Asparaginase (ASNase; EC, 3.5.1.1) catabolizes asparagine into aspartate and ammonium; therefore, it is suggested to play a key role in asparagine metabolism within legume sink organs. However, the metabolic fate of asparagine in source and sink organs during rice seed production remains to be elucidated. Therefore, the main objective of this study is to investigate the asparagine metabolism in a temporal and spatial manner during rice seed production.ResultsFor this purpose, the expression of genes involved in asparagine catabolism, such as asparaginase1 (OsASNase1) and 2 (OsASNase2), were quantitatively measured, and contents of asparagine, aspartate and ammonium ions were determined in sink and source organs during spikelet ripening. Quantitative real-time PCR and in situ localization studies determined that OsASNase2 is expressed in the dorsal vascular bundles and nucellar projection of developing grains, as well as in mesophyll and phloem companion cells of senescent flag leaves. Amino acid measurements revealed that the aspartate concentration is higher than asparagine in both source and sink organs.ConclusionThis work suggests that asparaginase dependent asparagine catabolism occurred not only in sink but also in source organs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-017-0143-8) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains

Yabuki

Ohashi

Imagawa

et al. 2017

Rice

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“…We also found ferredoxin-dependent glutamate synthase 1 (Fd-GOGAT) in this group. In Arabidopsis thaliana, there are two ferredoxin-or NADH-dependent GOGAT participating in the assimilation of ammonium into glutamate in leaves and roots, respectively [58]. Finally, LL-diaminopimelate aminotransferase, which is involved in the biosynthesis of lysine in plants by catalyzing the interconversion of the tetrahydrodipicolinate and the LLdiaminopimelate [59], has been also detected only in Wt samples.…”

Section: Proteins Involved In the Metabolism Of Amino Acidsmentioning

confidence: 99%

Characterization of the Arabidopsis thaliana 2-Cys peroxiredoxin interactome

Cerveau

Kraut

Stotz³

et al. 2016

Plant Science

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Peroxiredoxins are ubiquitous thiol-dependent peroxidases for which chaperone and signaling roles have been reported in various types of organisms in recent years. In plants, the peroxidase function of the two typical plastidial 2-Cys peroxiredoxins (2-Cys PRX A and B) has been highlighted while the other functions, particularly in ROS-dependent signaling pathways, are still elusive notably due to the lack of knowledge of interacting partners. Using an ex vivo approach based on co-immunoprecipitation of leaf extracts from Arabidopsis thaliana wild-type and mutant plants lacking 2-Cys PRX expression followed by mass spectrometry-based proteomics, 158 proteins were found associated with 2-Cys PRXs. Already known partners like thioredoxin-related electron donors (Chloroplastic Drought-induced Stress Protein of 32kDa, Atypical Cysteine Histidine-rich Thioredoxin 2) and enzymes involved in chlorophyll synthesis (Protochlorophyllide OxidoReductase B) or carbon metabolism (Fructose-1,6-BisPhosphatase) were identified, validating the relevance of the approach. Bioinformatic and bibliographic analyses allowed the functional classification of the identified proteins and revealed that more than 40% are localized in plastids. The possible roles of plant 2-Cys PRXs in redox signaling pathways are discussed in relation with the functions of the potential partners notably those involved in redox homeostasis, carbon and amino acid metabolisms as well as chlorophyll biosynthesis.

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“…In this contribution, the avoidance of a degradative phase during the process of N extraction in tissues [35,36] may be the explanation of tolerance to NH 4 , maintaining the concentration of free cytosolic NH 4 at sub-millimolar levels attributed to NH 4 detoxifying [5,23,37]. This analytical precaution was used in the preparation of the shoot and root of Oryza sativa (rice) juvenile plants, registering micromolar NH 4 accumulation [38] and showing the ability to assimilate this ion by means of the transaminases glutamate synthases ferredoxin dependents in leaf, and reduced nicotinamide adenine dinucleotide in root [39]. Although, submerged plants can present dual assimilation of NO 3 in leaf and NH 4 in root, they require less energy for uptake and assimilation NH 4 mainly because NO 3 has to be reduced prior to assimilation [30].…”

Section: Resultsmentioning

confidence: 99%

Tolerance to Nitrogen ions enrichment in a Gulf of Mexico's freshwater submerged grass

Ruiz-Carrera

Sánchez

Leal

2020

Preprint

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BackgroundTolerance to the enrichment of ionic nitrogen (N) with ammonium (NH 4 ) and nitrate (NO 3 ) needs to be distinguished in a submerged grass ecotype-species of freshwater wetlands. Concentrations of total Nitrogen (TN: 500 to 2000 µgL -1 ) and three N sources (NS: NH 4 , NO 3 , 1:1 NH 4 :NO 3 ratio) in an overlaying aqueous phase, using local tap water as control (174 µgL -1 TN), were evaluated in rooted autotrophic juvenile Vallisneria americana established in vitro in two-phase culture medium. The treatments ranged from 7 to 111 µM TN. Phenotypes changes, pH and ionic strength in the aqueous phase were registered at 15 days. In addition, biomass of lyophilized leaf and root as well as N contents (gas and high-performance liquid chromatography) were analysed to estimate accumulations of N-NH 4 , N-NO 3 and N-NO 2 .ResultsAll individuals showed phenotypic similarities. Accumulation of N-NH 4 was discarded, but the accumulations of N-NO 3 and N-NO 2 in both tissues were significantly different (p<0.001 and 0.0001), revealing so much that the maximum cumulative responses coincided with a deficit of N as both showed a linear decrease in their cumulative capacity linked to an increase in micromolar TN. ConclusionsThe results provide direct evidence of the tolerance to N enrichment with three different N sources in the focal ecotype at the seedling stage, based mainly on its cumulative capacity of N-NO 3 and N-NO 2 under eutrophic conditions. The findings imply that NO 2 is a relevant ion in the tolerance to N enrichment in overlaying water with NH 4 and NO 3 ions. Future studies of N cumulative effect with an in vitro approximation will be to support stress predictions by N enrichment. Keywords : Hydrocharitaceae. Eutrophization. Stress in vitro. Ammonium. Nitrate. Nitrogen endogenous

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NADH-dependent glutamate synthase participated in ammonium assimilation inArabidopsisroot

Cited by 19 publications

References 25 publications

A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains

A temporal and spatial contribution of asparaginase to asparagine catabolism during development of rice grains

Characterization of the Arabidopsis thaliana 2-Cys peroxiredoxin interactome

Tolerance to Nitrogen ions enrichment in a Gulf of Mexico's freshwater submerged grass

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