NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts

Nosek, Jozef; Fukuhara, Hiroshi

doi:10.1128/jb.176.18.5622-5630.1994

Cited by 72 publications

(51 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…mtDNA was analyzed by pulsed-field gel electrophoresis (PFGE) essentially as described previously (59). Briefly, whole-cell DNA samples were prepared in agarose plugs containing Zymolyase 20T (final concentration, 0.5 mg/ml; Seikagaku Corp.) and were incubated overnight in solution I (10 mM Tris-HCl, 0.45 M EDTA-NaOH [pH 8.8], 7.5% [vol/vol] 2-mercaptoethanol) at 37°C.…”

Section: Microbialmentioning

confidence: 99%

The Strictly Aerobic Yeast Yarrowia lipolytica Tolerates Loss of a Mitochondrial DNA-Packaging Protein

et al. 2014

View full text Add to dashboard Cite

bMitochondrial DNA (mtDNA) is highly compacted into DNA-protein structures termed mitochondrial nucleoids (mt-nucleoids). The key mt-nucleoid components responsible for mtDNA condensation are HMG box-containing proteins such as mammalian mitochondrial transcription factor A (TFAM) and Abf2p of the yeast Saccharomyces cerevisiae. To gain insight into the function and organization of mt-nucleoids in strictly aerobic organisms, we initiated studies of these DNA-protein structures in Yarrowia lipolytica. We identified a principal component of mt-nucleoids in this yeast and termed it YlMhb1p (Y. lipolytica mitochondrial HMG box-containing protein 1). YlMhb1p contains two putative HMG boxes contributing both to DNA binding and to its ability to compact mtDNA in vitro. Phenotypic analysis of a ⌬mhb1 strain lacking YlMhb1p resulted in three interesting findings. First, although the mutant exhibits clear differences in mt-nucleoids accompanied by a large decrease in the mtDNA copy number and the number of mtDNA-derived transcripts, its respiratory characteristics and growth under most of the conditions tested are indistinguishable from those of the wild-type strain. Second, our results indicate that a potential imbalance between subunits of the respiratory chain encoded separately by nuclear DNA and mtDNA is prevented at a (post)translational level. Third, we found that mtDNA in the ⌬mhb1 strain is more prone to mutations, indicating that mtHMG box-containing proteins protect the mitochondrial genome against mutagenic events. Individual eukaryotic cells contain a population of mitochondrial DNA (mtDNA) molecules, the number of which may reach several thousand copies. For example, aerobically grown diploid cells of the yeast Saccharomyces cerevisiae contain, on average, 100 molecules of 85-kbp mtDNA. With a distance of 0.34 nm between base pairs in DNA, the total length of mtDNA reaches almost 3 mm per cell, while the diameter of the cell does not exceed 3 to 4 m. Analogously to its nuclear counterpart, mtDNA must be packaged into condensed nucleoprotein structures termed mitochondrial nucleoids (mt-nucleoids) (1-6). The size of mt-nucleoids in S. cerevisiae ranges from 0.2 to 0.9 m, meaning that mtDNA in yeasts undergoes compaction of roughly 3 orders of magnitude. Although it is known that the size and shape (oval versus globular) of mt-nucleoids, the number of mt-nucleoids (ranging from 50 to 70) per diploid cell (2), and the number of copies (up to 9) of mtDNA per mt-nucleoid (3) in S. cerevisiae depend on physiological conditions, the molecular mechanisms mediating nucleoid maintenance, dynamics, and roles in mtDNA distribution/segregation during cell division are largely not understood. This is also true for mammalian mt-nucleoids, which, in contrast to their yeast counterparts, contain a relatively small number of mtDNA molecules and are thus more solitary in nature (7,8). Description of these intra-and interspecific differences in the organization of mt-nucleoids would greatly facilitate our understanding of the m...

show abstract

Section: Microbialmentioning

confidence: 99%

The Strictly Aerobic Yeast Yarrowia lipolytica Tolerates Loss of a Mitochondrial DNA-Packaging Protein

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Pulsed Field Gel Electrophoresis-DNA samples were prepared as described previously (16). Briefly, yeast cells were lysed in agarose blocks by sequential treatment with zymolyase and proteinase K. Samples were electrophoretically separated in a 0.8% agarose gel in 0.5ϫ TBE (45 mM Tris borate, 1 mM EDTA) buffer in a Pulsaphor apparatus (LKB) in contour-clamped homogeneous electric field configuration.…”

Section: Preparation Of Mitochondrial Protein Extracts and Purificatimentioning

confidence: 99%

Mitochondrial Telomere-binding Protein from Candida parapsilosis Suggests an Evolutionary Adaptation of a Nonspecific Single-stranded DNA-binding Protein

Nosek

Tomáška

Pagác̆ová

et al. 1999

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Next, the gene contents in both mtDNAs were inferred by sequencing the termini of the restriction fragments inserted within the plasmid vector. The data were then compared with the GenBank database as well as with the complete sequence of mtDNA of the group I strain CBS 7157 (Nosek & Fukuhara, 1994a Tomaska, unpublished). This approach led to the identification of nad1-5, cytb, atp6 and rrnL genes in MCO 456 and nad1-5, cytb, cox1, cox3, atp6 and rrnL in MCO 448.…”

Section: Physical Mapping Of Mtdna In Different Groups Of C Parapsilmentioning

confidence: 99%

Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis

et al. 2004

View full text Add to dashboard Cite

The yeast species Candida parapsilosis, an opportunistic pathogen, exhibits genetic and genomic heterogeneity. To assess the polymorphism at the level of mitochondrial DNA (mtDNA), the organization of the mitochondrial genome in strains belonging to the three variant groups of this species was investigated. Although these analyses revealed a group-specific restriction fragment pattern of mtDNA, strains belonging to different groups appear to have similar genes in the same gene order. An extensive survey of C. parapsilosis isolates uncovered surprising alterations in the molecular architecture of their mitochondrial genome. A screening strategy for strains harbouring mtDNA with rearranged architecture showed that nearly all strains from groups I and III possess linear mtDNA molecules terminating with arrays of tandem repeat units, while most of the group II strains have a circular mitochondrial genome. In addition, it was found that linear genophores in mitochondria of strains from different groups differ in the sequence of the mitochondrial telomeric repeat unit. The occurrence of altered forms of mtDNA among C. parapsilosis strains opens up the unique possibility to address questions concerning the evolutionary origin and replication strategy of linear and circular genomes in mitochondria.

show abstract

NADH dehydrogenase subunit genes in the mitochondrial DNA of yeasts

Cited by 72 publications

References 58 publications

The Strictly Aerobic Yeast Yarrowia lipolytica Tolerates Loss of a Mitochondrial DNA-Packaging Protein

The Strictly Aerobic Yeast Yarrowia lipolytica Tolerates Loss of a Mitochondrial DNA-Packaging Protein

Mitochondrial Telomere-binding Protein from Candida parapsilosis Suggests an Evolutionary Adaptation of a Nonspecific Single-stranded DNA-binding Protein

Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis

Contact Info

Product

Resources

About