Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq

Hamoo, Reedha N.; Mustafa, Nashaat G.; Abdulraheem, Samar

doi:10.33899/ijvs.2019.162965

Cited by 3 publications

(3 citation statements)

References 21 publications

(26 reference statements)

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“…These finding suggests that the sheep-dog cycle occur in this region. However, genotyping of sheep hydatid cysts using sequencing with cox1 gene, G1 is described as predominant genotype in various countries such as Peru (18), Egypt (19) China, Turkey, Italy and Tunisia that are consistent with this study (20)(21)(22) and with highly remarkable corresponding to north African and Mediterranean countries isolates (23). Genotype of E. granulosus, mitochondrial genes are much better than other genome sources for E. granulosus genotyping.…”

Section: Discussionsupporting

confidence: 84%

Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors

Abdulla¹,

Mageed²,

Obed³

et al. 2020

IJVS

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The aim of the study is characterizing of hydatid cysts that have been isolated from sheep and cow liver fertile hydatid cysts using mitochondrial cytochrome c oxidase subunit 1(cox1) gene. DNA samples of nineteen sheep and one cow were extracted from protoscoleces and germinal layers of the parasites in Koya city-Erbil, Iraq, using specific extraction procedures. Mitochondrial cox1 gene region was amplified by polymerase chain reaction (PCR), and the expected gene sizes were confirmed by gel electrophoresis. All DNA isolates were then sequenced. Nucleotide sequence alignments were then performed to verify the sequenced isolated according to the database, which showed that all samples were belonging to the (G1) sheep strain. Phylogenetic analysis was also carried out for the sequenced isolated to find out the highest similarities with closest organisms to E. granulosus' conserved gene and to reveal sharing common ancestor, which has been confirmed. Electrocatalytic reduction of DNA where detected through applying cyclic-voltammetry technique, which referred to the environmentally strong protection features of these strains against any effects of external factors, such as heavy metals and has revealed the secret behind the potent preservation of the DNA structure of this parasite from being affect by mutations, or alterations, along the different lineages over a long period of time.

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Section: Discussionsupporting

confidence: 84%

Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors

Abdulla¹,

Mageed²,

Obed³

et al. 2020

IJVS

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“…Various diagnostic approaches were employed to differentiate pathogenic parasites of human and animals, including Fasciola, but at least until now, molecular protocols are the best, the precise, and fully future (15)(16)(17). This study, as the knowledge of the authors, is the first in Iraq that utilized an 18S rRNA gene of F. gigantica in the confirmed diagnosis and differentiation with global isolates which documented in NCBI and GenBank.…”

Section: Discussionmentioning

confidence: 89%

Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Hamoo¹,

Al-Rubaye²,

Mustafa³

2020

IJVS

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This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq. Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol. Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products. A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No. MG786553). However, it was confirmed by the sequence results that isolate species was F. gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations. It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise. Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F. gigantica, also our samples have 100% alignment match with universal isolates.

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“…The results demonstrated in this investigation agree with Iraqi isolates. Hamoo and Abdulraheem (2019) showed that the local isolate is G1 genotype (sheep strain), and the phylogenetic results were compatible with North Africa and the Middle East isolates as 95% and 96%. Fadhil & A'aiz (2016) reported three strains of the E.granulosus parasite, sheep strain (G1) 40%, buffalo strain (G3) 48% and camel strain (G6) 12%.…”

Section: Discussionmentioning

confidence: 52%

Determination genetic variation and phylogenetic analysis in Echinococcus granulosus isolated from Iraqi sheep

Bayraktar

Hussein²,

Hassan³

2022

J Hellenic Vet Med Soc

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Echinococcus granulosus causes Cystic Echinococcosis disease in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Cystic Echinococcosis is an essential parasitic zoonosis that affects humans and animals and is a significant public health and economic problem worldwide. This study aimed to determine the molecular characterization and phylogenetic analysis of Echinococcus granulosus in sheep's livers and lungs. The genetic variation was performed by directly sequencing the mitochondrial DNA (mtDNA) genes coding of the NADH dehydrogenase subunit 1 (ND1). The evidence from molecular studies and DNA sequencing revealed that the Isolates of Echinococcus granulosus in Kirkuk city related to the sheep strain G1 genotype. The alignment analysis showed a concordance between isolates with each other by 99%. When comparing the results with Genbank, it indicated the occurrence of genetic variations between nucleotides represented by eleven codons, seven of which represent transversion mutations, and four are transition mutations.

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Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq

Cited by 3 publications

References 21 publications

Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors

Molecular characterization of fertile hydatid cysts from the liver of the sheep and cows and associated environmental influence factors

Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Determination genetic variation and phylogenetic analysis in Echinococcus granulosus isolated from Iraqi sheep

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