NAD+ precursor increases aerobic performance in mice

Crisol, Barbara Moreira; Veiga, Camilla Bertuzzo; Braga, Renata Rosseto; Lenhare, Luciene; Baptista, Igor L.; Gaspar, Rafael Calais; Muñoz, Vitor Rosetto; Cordeiro, André V.; Silva, Adelino Sánchez Ramos da; Cintra, Dennys Esper; Moura, Leandro Pereira de; Pauli, José Rodrigo; Ropelle, Eduardo Rochete

doi:10.1007/s00394-019-02089-z

Cited by 26 publications

(27 citation statements)

References 34 publications

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“…A human study [ 34 ] of NR on body composition in healthy obese middle and older people showed that body fat % improved, whereas body weight remained unchanged, and the improvement appeared to be a gender-dependent. Exercise combined with NR showed no additional improvement in body weight reduction and fat deposits in young mice compared to exercise only [ 27 ]. Considering the good standing of BMI and body fat % of the participants in this study, no further changes in BMI and body fat % were expected.…”

Section: Discussionmentioning

confidence: 99%

“…NMN supplementation had been reported to reduce post-exercise blood lactate with improvement in endurance in mice [ 17 ]. Another research study showed that three-week swimming training combined NR administration (300 mg/kg/d) reduced glucose concentration and maximal blood lactate accumulation in rats with a decrease in exercise endurance [ 27 ]. The measurement of blood lactate could provide another insight into the metabolic adaptations underlying the VT-improving effect.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, overexpression of muscle NAMPT in combination with exercise in wild-type mice augmented exercise endurance, maximum oxygen uptake (VO 2 max), and mitochondrial respiratory capacity compared with exercise training alone [ 23 , 26 ]. NR (400 mg/kg/d) and NMN (500 mg/kg/d) with exercise training increased endurance performance in healthy young [ 27 ] and elderly [ 17 ] mice. Nevertheless, a decrease in endurance performance from NR (300 mg/kg/d) in combination with swimming training was shown in young rats [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study

Liao

Zhao

Wang

et al. 2021

Journal of the International Society of Sports Nutrition

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Background Recent studies in rodents indicate that a combination of exercise training and supplementation with nicotinamide adenine dinucleotide (NAD+) precursors has synergistic effects. However, there are currently no human clinical trials analyzing this. Objective This study investigates the effects of a combination of exercise training and supplementation with nicotinamide mononucleotide (NMN), the immediate precursor of NAD+, on cardiovascular fitness in healthy amateur runners. Methods A six-week randomized, double-blind, placebo-controlled, four-arm clinical trial including 48 young and middle-aged recreationally trained runners of the Guangzhou Pearl River running team was conducted. The participants were randomized into four groups: the low dosage group (300 mg/day NMN), the medium dosage group (600 mg/day NMN), the high dosage group (1200 mg/day NMN), and the control group (placebo). Each group consisted of ten male participants and two female participants. Each training session was 40–60 min, and the runners trained 5–6 times each week. Cardiopulmonary exercise testing was performed at baseline and after the intervention, at 6 weeks, to assess the aerobic capacity of the runners. Results Analysis of covariance of the change from baseline over the 6 week treatment showed that the oxygen uptake (VO2), percentages of maximum oxygen uptake (VO2max), power at first ventilatory threshold, and power at second ventilatory threshold increased to a higher degree in the medium and high dosage groups compared with the control group. However, there was no difference in VO2max, O2-pulse, VO2 related to work rate, and peak power after the 6 week treatment from baseline in any of these groups. Conclusion NMN increases the aerobic capacity of humans during exercise training, and the improvement is likely the result of enhanced O2 utilization of the skeletal muscle. Trial registration number ChiCTR2000035138.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study

Liao

Zhao

Wang

et al. 2021

Journal of the International Society of Sports Nutrition

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“…However, studies have shown limited and inconclusive results for body composition and metabolism ( 11 , 47 ). Further, few studies have combined NR supplementation with standard weight loss practices; only four have been found published (three in rodents and one in humans), all associated solely with physical activity ( 27 , 48 – 50 ). Additionally, evidence for its effect on muscle tissue from obese/overweight individuals follows the same dilemma.…”

Section: Discussionmentioning

confidence: 99%

“…NAD is a key coenzyme for muscle cells, both for energy metabolism and muscle contraction (25). Furthermore, it serves as a co-substrate for post-translational modifications (for example, mono/poly-ADP-ribosylation and deacetylation) that influence the health and maintenance of SMT (25)(26)(27)(28). We recognize that the lack of histological data, particularly to determine the cross-sectional area of skeletal muscle fibers, and of molecular data, aiming to quantify NAD and its derivatives, are limitations of this study.…”

Section: Discussionmentioning

confidence: 99%

Nicotinamide Riboside Neutralizes Hypothalamic Inflammation and Increases Weight Loss Without Altering Muscle Mass in Obese Rats Under Calorie Restriction: A Preliminary Investigation

et al. 2021

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Obesity treatments, such as calorie restriction (CR), eventually lead to muscle wasting and higher rates of neuroinflammation, whereas hypothalamic inflammatory conditions impair body weight (BW) control. Nicotinamide riboside (NR) has been proposed against obesity but with little evidence on skeletal muscle tissue (SMT) and neuroinflammation. Therefore, we aimed to investigate the effects of CR on SMT and on hypothalamic inflammatory biomarkers in obese adult male Wistar rats, and whether NR supplementation alone or in combination with CR affects these parameters. Obesity was induced in rats through a cafeteria diet for 6 weeks. After that, a group of obese rats was exposed to CR, associated or not associated with NR supplementation (400 mg/kg), for another 4 weeks. As a result, obese rats, with or without CR, presented lower relative weight of SMT when compared with eutrophic rats. Rats under CR presented lower absolute SMT weight compared with obese and eutrophic rats, in addition to presenting elevated hypothalamic levels of TNF-α. NR supplementation, in all groups, enhanced weight loss and increased relative weight of the SMT. Furthermore, in animals under CR, NR reversed increases TNF-α levels in the hypothalamus. In this study, these data, although succinct, are the first to evidence the effects of NR on SMT and neuroinflammation when associated with CR, especially in obesity conditions. Therefore, this provides preliminary support for future studies in this investigative field. Furthermore, NR emerges as a potential adjuvant for preventing muscle mass loss in the weight loss processes.

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Aging reduces ABHD5 protein content in the adipose tissue of mice: The reversal effect of exercise

Brícola

Cordeiro

Crisol

et al. 2022

Cell Biochemistry & Function

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Dysfunction of the adipose tissue metabolism is considered as a significant hallmark of aging. It has been proposed that α-β hydrolase domain containing 5 (ABHD5) plays a critical role in the control of lipolysis. However, the role of ABHD5 in the control of lipolysis during aging or exercise is unknown. Here we combined the experimental mouse model with transcriptomic analyzes by using murine and human databases to explore the role of ABHD5 in the adipose tissue during aging and in response to exercise. Transcriptomic data revealed a downregulation of Abhd5 messenger RNA levels in the subcutaneous white adipose tissue (scWAT) over time in individuals from 20 to 69 years old. Aged mice displayed dramatic reduction of ABHD5 protein content and lipolytic-related proteins in the scWAT. Interestingly, 4 weeks of high-intensity interval training increased ABHD5 protein level and restored the lipolytic pathway in the scWAT of aged mice. Altogether, our findings demonstrated that aging affects ABHD5 content in the adipose tissue of mice and humans. Conversely, exercise increases ABHD5 activity, recovering the lipolytic activity in aged mice.

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NAD+ precursor increases aerobic performance in mice

Cited by 26 publications

References 34 publications

Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study

Nicotinamide mononucleotide supplementation enhances aerobic capacity in amateur runners: a randomized, double-blind study

Nicotinamide Riboside Neutralizes Hypothalamic Inflammation and Increases Weight Loss Without Altering Muscle Mass in Obese Rats Under Calorie Restriction: A Preliminary Investigation

Aging reduces ABHD5 protein content in the adipose tissue of mice: The reversal effect of exercise

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