Nacre Topography Produces Higher Crystallinity in Bone than Chemically Induced Osteogenesis

Alakpa, Enateri V.; Burgess, Karl; Chung, Peter; Riehle, Mathis O.; Gadegaard, Nikolaj; Dalby, Matthew J.; Cusack, Maggie

doi:10.1021/acsnano.7b01044

Cited by 42 publications

(39 citation statements)

References 89 publications

(167 reference statements)

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“…The transition from disordered ACP to ordered HAP is observed with increased crystallite c-axis length and improvement in the stoichiometric organization of the crystal lattice. 54,96 Subsequently, reduction in shortrange disorder and progressive homogeneity of the mineral constituents have shown to result in a narrowing of the bandwidth, 60 as observed for the HC architecture with respect to the NT conditions. Taken together, our Raman results show that the HC architecture induces more advanced mineralization of crystalline hydroxyapatite nodules when compared to the other conditions.…”

Section: -mentioning

confidence: 90%

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<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

Steeves¹,

Ho²,

Munisso³

et al. 2020

IJN

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Introduction: In recent years there has been ample interest in nanoscale modifications of synthetic biomaterials to understand fundamental aspects of cell-surface interactions towards improved biological outcomes. In this study, we aimed at closing in on the effects of nanotubular TiO 2 surfaces with variable nanotopography on the response on human mesenchymal stem cells (hMSCs). Although the influence of TiO 2 nanotubes on the cellular response, and in particular on hMSC activity, has already been addressed in the past, previous studies overlooked critical morphological, structural and physical aspects that go beyond the simple nanotube diameter, such as spatial statistics. Methods: To bridge this gap, we implemented an extensive characterization of nanotubular surfaces generated by anodization of titanium with a focus on spatial structural variables including eccentricity, nearest neighbour distance (NND) and Voronoi entropy, and associated them to the hMSC response. In addition, we assessed the biological potential of a twotiered honeycomb nanoarchitecture, which allowed the detection of combinatory effects that this hierarchical structure has on stem cells with respect to conventional nanotubular designs. We have combined experimental techniques, ranging from Scanning Electron (SEM) and Atomic Force (AFM) microscopy to Raman spectroscopy, with computational simulations to characterize and model nanotubular surfaces. We evaluated the cell response at 6 hrs, 1 and 2 days by fluorescence microscopy, as well as bone mineral deposition by Raman spectroscopy, demonstrating substrate-induced differential biological cueing at both the short-and long-term. Results: Our work demonstrates that the nanotube diameter is not sufficient to comprehensively characterize nanotubular surfaces and equally important parameters, such as eccentricity and wall thickness, ought to be included since they all contribute to the overall spatial disorder which, in turn, dictates the overall bioactive potential. We have also demonstrated that nanotubular surfaces affect the quality of bone mineral deposited by differentiated stem cells. Lastly, we closed in on the integrated effects exerted by the superimposition of two dissimilar nanotubular arrays in the honeycomb architecture. Discussion: This work delineates a novel approach for the characterization of TiO 2 nanotubes which supports the incorporation of critical spatial structural aspects that have been overlooked in previous research. This is a crucial aspect to interpret cellular behaviour on nanotubular substrates. Consequently, we anticipate that this strategy will contribute to the unification of studies focused on the use of such powerful nanostructured surfaces not only for biomedical applications but also in other technology fields, such as catalysis.

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Section: -mentioning

confidence: 90%

“…Mineral crystallinity, which depends on the disorder in the bone structure, was calculated from the 1/FWHM of the phosphate ν1 band (960 cm −1 ). [59][60][61][62]…”

Section: Raman Spectroscopymentioning

confidence: 99%

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

Steeves¹,

Ho²,

Munisso³

et al. 2020

IJN

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“…Calcium carbonate is the main component of mollusk shells. In a similar way to bone, shells are formed by an organic/inorganic composite with a complex macro and microscopic structure responsible for its exceptional mechanical properties (Alakpa et al, 2017;Li, Xin , 2009). Calcium carbonates possess better biodegradation rates than HAp while maintaining a suitable mechanical strength which make them appropriate for bone regeneration (Yang et al, 2016).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

Díaz‐Rodríguez

García‐Triñanes

López

et al. 2018

Carbohydrate Polymers

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The search for an ideal bone tissue replacement has led to the development of new composite materials designed to simulate the complex inorganic/organic structure of bone. The present work is focused on the development of mineralized calcium alginate hydrogels by the addition of marine derived calcium carbonate biomineral particles. Following a novel approach, we were able to obtain calcium carbonate particles of high purity and complex micro and nanostructure dependent on the source material. Three different types of alginates were selected to develop inorganic/organic scaffolds in order to correlate alginate composition with scaffold properties and cell behavior. The incorporation of calcium carbonates into alginate networks was able to promote extracellular matrix mineralization and osteoblastic differentiation of mesenchymal stem cells when added at 7 mg/ml. We demonstrated that the selection of the alginate type and calcium carbonate origin is crucial to obtain adequate systems for bone tissue engineering as they modulate the mechanical properties and cell differentiation.

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“…Fmoc-F 2 /S [42] pericyte chondrogenesis RADA-PRG (modified PuraMatrix w ) [43,44] mouse neonatal epidermal cells, rMSC neurogenesis RGD on decellularized pig heart valves [45] EPC proliferation Synthemax w [46] iPSC expansion and differentiation polymer enzymatically responsive PEG [47 -52] iPSC, hESC, mESC, MSC, organoids, mouse pancreatic progenitor gellan gum [53] hPSC 3D spheres methylcellulose [54] MSC chondrogenesis nanotopographically imprinted PCL [55,56] MSC maintenance and osteogenesis NO-releasing chitosan [57] hP-MSC angiogenic potential P(PEGMEMA-r-GMA-r-VDM) [58] MSC proliferation PEG with RGD and MMP tethering [59,60] MSC migration photodegradable PEG [61,62] MSC 'mechanical memory' polyacrylate/acrylamide thermoresponsive hydrogel [63 -65] hESC, MSC, mESC polyacrylate/polyurethane [66 -68] hESC, MSC, C6 rat GSC polypyrrole [69] MSC osteogenesis polystyrene TopoChip [70] iPSC proliferation polyurethane [71 -74] hESC-derived HE, HPC, iPSC-derived hepatocytes, H9 ternary polymer blends [75,76] STRO-1 þ skeletal SC, fetal skeletal SC rstb.royalsocietypublishing.org Phil. Trans.…”

Section: Protein-based Substrates For Stem Cell Controlmentioning

confidence: 99%

New substrates for stem cell control

Schmidt¹,

Lilienkampf²,

Bradley³

2018

Phil. Trans. R. Soc. B

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The capacity to culture stem cells in a controllable, robust and scalable manner is necessary in order to develop successful strategies for the generation of cellular and tissue platforms for drug screening, toxicity testing, tissue engineering and regenerative medicine. Creating substrates that support the expansion, maintenance or directional differentiation of stem cells would greatly aid these efforts. Optimally, the substrates used should be chemically defined and synthetically scalable, allowing growth under defined, serum-free culture conditions. To achieve this, the chemical and physical attributes of the substrates should mimic the natural tissue environment and allow control of their biological properties. Herein, recent advances in the development of materials to study/manipulate stem cells, both and are described with a focus on the novelty of the substrates' properties, and on application of substrates to direct stem cells.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.

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Nacre Topography Produces Higher Crystallinity in Bone than Chemically Induced Osteogenesis

Cited by 42 publications

References 89 publications

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

Mineralized alginate hydrogels using marine carbonates for bone tissue engineering applications

New substrates for stem cell control

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