NaCl stress effects on enzymes involved in nitrogen assimilation pathway in tomato “Lycopersicon esculentum” seedlings

Debouba, Mohamed; Gouia, Houda; Suzuki, Akira; Ghorbel, Mohamed Habib

doi:10.1016/j.jplph.2005.09.012

Cited by 218 publications

(145 citation statements)

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“…4B). This may result from the salt-induced increase in glutamine synthetase and glutamate dehydrogenase activities in tomato roots (Debouba et al, 2006). Despite the salt induction of in vitro glutamate dehydrogenase activity in tomato, its physiological signifi-cance is not proved (Debouba et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…4D). Asparagine accumulation under abiotic stress was attributed to increasing asparagine synthetase activity (Chaffei et al, 2004) or protein catabolism (Debouba et al, 2006). Indeed, expression of HAS1 and HAS1.1 genes encoding for asparagine synthetase was induced by osmotic stress (300 mM mannitol), salt stress (150 mM NaCl), and heavy-metal stress (20 μM CuSO 4 ), more in roots than in leaves of sunflower (Herrera-Rodríguez et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Aminoacid changes in leaves and roots of tomato(Solanum lycopersicum)during salt stress

Debouba¹,

Suzuki

Boutet

et al. 2010

Acta Botanica Gallica

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Abstract.-Tomato seedlings were exposed to 100 mM NaCl during 10 days. NaCl stress led to about 40% and 20% decrease in leaf and root biomass, respectively. Accumulation of Na + and Cl -ions was concomitant with NH 4 + contents increase in stressed leaves and roots. While total aminoacids contents rapidly increased by salt in both organs, they recovered control value in the leaves at the end of treatment. In leaves, NaCl stress increased proline and serine contents, lately associated with accumulation of γ-aminobutyric and asparagine. Slat-treated roots showed 2-fold higher contents of serine, glycine, glutamine and GABA. Asparagine became the most abundant aminoacid, accounting for 26% of total aminoacids in the treated roots. These disturbances in the aminoacids composition reflect a new distribution of internal nitrogen compounds, building up an aminoacid pool with high N to C ratio and avoiding thus an ammoniacal intoxication. Proline, Asn and GABA are useful for nitrogen transport to developing tissues and protect against osmotic and oxidative stresses during salt stress.Key words : tomato -salt stress -growth -aminoacids.Résumé.-De jeunes plants de tomate ont été exposés à 100 mM NaCl pendant 10 jours. Le stress salin provoque une diminution d'environ 40% et 20% respectivement de la biomasse foliaire et racinaire. L'accumulation endogène des ions Na + et Cl -est associée à une accélération de la production des ions NH 4 + dans les tissus foliaires et racinaires des plantes stressées. Les teneurs en acides aminés totaux sont augmentées par la salinité dans les racines. Au niveau des feuilles, le stress a provoqué une augmentation rapide des teneurs en proline et en sérine, associée tardivement à une accumulation de l'acide γ-aminobutyrique et de l'asparagine. En même temps, les racines traitées ont montré des teneurs environ 2 fois plus élevées en sérine, en glycine, en glutamine et en GABA relativement aux témoins. Ces perturbations dans la composition en acides aminés reflètent une tendance de la plante à stocker NH 4 + sous forme d'acides aminés afin d'éviter une intoxication ammoniacale. De même, la Pro et le GABA peuvent intervenir dans la lutte contre les stress osmotique et oxydatif associés au stress salin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Aminoacid changes in leaves and roots of tomato(Solanum lycopersicum)during salt stress

Debouba¹,

Suzuki

Boutet

et al. 2010

Acta Botanica Gallica

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“…Absorbance of the released-azo-dye-was measured at 340 nm and one unit of activity was defined as the activity producing an increase of 0.01 units of absorbance during 1 h incubation. 48 …”

Section: Methodsmentioning

confidence: 99%

Prolonged root hypoxia effects on enzymes involved in nitrogen assimilation pathway in tomato plants

Horchani

Aschi‐Smiti²

2010

Plant Signaling & Behavior

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IntroductionPlants grow in a dynamic environment, which frequently imposes constraints on growth and development. Among the adverse environmental factors commonly encountered by land plants, flooding is one of the most significant abiotic stresses. 1 Flooding of the soil can have a tremendous impact on the growth and survival of plants, and thereby on agricultural as well as natural ecosystems. In the last decades considerable progress has been made in our understanding of the mechanisms that enable certain plant species and cultivars to withstand periods with excess soil water or even complete submergence. Much of the research has been carried out with crop plant species, such as Oryza sativa, Zea mays, Trifolium subterraneum and Medicago sativa, 2-5 but wild species originating from wetland habitats have been also used, mostly for comparative studies. 6,7 With transient flooding or irrigation followed by slow drainage, or in natural wetlands, plant roots can become oxygen deficient because of slow transfer of dissolved oxygen in the waterfilled pore space of the soil. The decrease in ambient oxygen may not be, itself or alone, the perceived signal but could trigger metabolic responses, which then initiate the signalling cascade. 8 The immediate consequence of oxygen depletion is the reduction in respiration, thus diminishing ATP generation, and leading to a decrease in the ATP/ADP ratio and the adenylate energy charge. 9 In order to investigate the effects of root hypoxia (1-2% oxygen) on the nitrogen (N) metabolism of tomato plants (Solanum lycopersicum L. cv. Micro-Tom), a range of N compounds and N-assimilating enzymes were performed on roots and leaves of plants submitted to root hypoxia at the second leaf stage for three weeks. Obtained results showed that root hypoxia led to a significant decrease in dry weight (DW) production and nitrate content in roots and leaves. conversely, shoot to root DW ratio and nitrite content were significantly increased. contrary to that in leaves, glutamine synthetase activity was significantly enhanced in roots. The activities of nitrate and nitrite reductase were enhanced in roots as well as leaves. The higher increase in the Nh 4 + content and in the protease activities in roots and leaves of hypoxically treated plants coincide with a greater decrease in soluble protein contents. Taken together, these results suggest that root hypoxia leaded to higher protein degradation. The hypoxia-induced increase in the aminating glutamate dehydrogenase activity may be considered as an alternative N assimilation pathway involved in detoxifying the Nh 4 + , accumulated under hypoxic conditions. With respect to hypoxic stress, the distinct sensitivity of the enzymes involved in N assimilation is discussed. and 0.13 ± 0.03 g, respectively. The changes in the root and shoot dry weights (DW) and in shoot/root ratio were investigated after a three week period of hypoxic treatment. Root hypoxia decreased root and leaf DW production by 34 and 18%, respectively. Shoot to root DW ratio increas...

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“…Moreover, inadequate nitrogen is often acts as a growthlimiting nutritional stress in field soils. N uptake, translocation, and assimilation as well as nitrogen natural isotopic signature appear to be especially sensitive to salinity [3].…”

Section: Effect Of Salt Stress On Nutritional Value Of Vegetablesmentioning

confidence: 99%

Effect of Salt Stress on Nutritional Value of Vegetables

Prasad¹,

Parihar²,

Singh³

2014

Biochem & Pharmacol

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Vegetables serve as a source of pharmacologically active molecules. However, due to increased environmental stresses the concentration of bioactive compounds and the valuable constituents of vegetables are getting deteriorated day by day. Salt stress is one of the major environmental stresses affecting the plants nutritional value. Salt stress influences the plant growth by inducing adverse effects on different physiological and biochemical processes and thus leads to nutrient disorder. Therefore, there is a need to develop some tools/techniques which would be helpful for the farmers whose soil or irrigation water are saline as well as those people who frequently consume vegetables for nutritional purposes.

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NaCl stress effects on enzymes involved in nitrogen assimilation pathway in tomato “Lycopersicon esculentum” seedlings

Cited by 218 publications

References 55 publications

Aminoacid changes in leaves and roots of tomato(Solanum lycopersicum)during salt stress

Aminoacid changes in leaves and roots of tomato(Solanum lycopersicum)during salt stress

Prolonged root hypoxia effects on enzymes involved in nitrogen assimilation pathway in tomato plants

Effect of Salt Stress on Nutritional Value of Vegetables

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